Isolation and cloning of two variant papillomaviruses from domestic pigs: papillomaviruses type 1 variants a and b Free

Abstract

The healthy skin of two female domestic pigs () was sampled with cotton-tipped swabs. Total genomic DNA was extracted from the samples and subjected to PCR with degenerate papillomavirus (PV)-specific primers. Similarity searches performed with showed that partial E1 and L1 sequences of two novel PVs were amplified. Subsequently, the complete genomes of these papillomaviruses (SsPVs) were amplified by long-template PCR, cloned and sequenced using a transposon insertion method. They contained the typical PV open reading frames (ORFs) E1, E2, E4, E6, L1 and L2, but the E7 ORF was absent in both viruses. Pairwise nucleotide sequence alignment of the L1 ORFs of the SsPVs showed 98.5 % similarity, classifying these viruses as SsPV type 1 ‘variants’ (SsPV-1a and -1b). Based on a concatenated alignment of the E1, E2, L1 and L2 ORFs of SsPV-1 variants a and b, and 81 other human and animal PV type species, a neighbour-joining phylogenetic tree was constructed. This phylogenetic analysis showed that the SsPV-1a and -1b variants did not cluster with the other PVs of artiodactyls (cloven-hoofed) host species, but clustered on the edge of the genus , very near to the root of this genus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/003186-0
2008-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/10/2475.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/003186-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Amtmann E., Muller H., Sauer G. 1980; Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. J Virol 35:962–964
    [Google Scholar]
  3. Antonsson A., Hansson B. G. 2002; Healthy skin of many animal species harbors papillomaviruses which are closely related to their human counterparts. J Virol 76:12537–12542 [CrossRef]
    [Google Scholar]
  4. Chan S. Y., Bernard H. U., Ratterree M., Birkebak T. A., Faras A. J., Ostrow R. S. 1997; Genomic diversity and evolution of papillomaviruses in rhesus monkeys. J Virol 71:4938–4943
    [Google Scholar]
  5. de Villiers E. M., Fauquet C., Broker T. R., Bernard H. U., zur Hausen H. 2004; Classification of papillomaviruses. Virology 324:17–27 [CrossRef]
    [Google Scholar]
  6. Hafner M. S., Nadler S. A. 1988; Phylogenetic trees support the coevolution of parasites and their hosts. Nature 332:258–259 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Moreno-Lopez J., Ahola H., Eriksson A., Bergman P., Pettersson U. 1987; Reindeer papillomavirus transforming properties correlate with a highly conserved E5 region. J Virol 61:3394–3400
    [Google Scholar]
  9. Ogawa T., Tomita Y., Okada M., Shinozaki K., Kubonoya H., Kaiho I., Shirasawa H. 2004; Broad-spectrum detection of papillomaviruses in bovine teat papillomas and healthy teat skin. J Gen Virol 85:2191–2197 [CrossRef]
    [Google Scholar]
  10. Parish W. E. 1961; A transmissible genital papilloma of the pig resembling condyloma acuminatum of man. J Pathol Bacteriol 81:331–345 [CrossRef]
    [Google Scholar]
  11. Rector A., Bossart G. D., Ghim S. J., Sundberg J. P., Jenson A. B., Van Ranst M. 2004; Characterization of a novel close-to-root papillomavirus from a Florida manatee by using multiply primed rolling-circle amplification: Trichechus manatus latirostris papillomavirus type 1. J Virol 78:12698–12702 [CrossRef]
    [Google Scholar]
  12. Rector A., Tachezy R., Van Doorslaer K., MacNamara T., Burk R. D., Sundberg J. P., Van Ranst M. 2005a; Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1. Virology 331:449–456 [CrossRef]
    [Google Scholar]
  13. Rector A., Van Doorslaer K., Bertelsen M., Barker I. K., Olberg R.-A., Lemey P., Sundberg J. P., Van Ranst M. 2005b; Isolation and cloning of the raccoon ( Procyon lotor ) papillomavirus type 1 by using degenerate papillomavirus-specific primers. J Gen Virol 86:2029–2033 [CrossRef]
    [Google Scholar]
  14. Rehtanz M., Ghim S. J., Rector A., Van Ranst M., Fair P. A., Bossart G. D., Jenson A. B. 2006; Isolation and characterization of the first American bottlenose dolphin papillomavirus: Tursiops truncatus papillomavirus type 2. J Gen Virol 87:3559–3565 [CrossRef]
    [Google Scholar]
  15. Stevens H., Rector A., Bertelsen M. F., Leifsson P. S., Van Ranst M. 2007; Novel papillomavirus isolated from the oral mucosa of a polar bear does not cluster with other papillomaviruses of carnivores. Vet Microbiol 129:108–116
    [Google Scholar]
  16. Sundberg J. P. 1987; Papillomavirus infections in animals. In Papillomaviruses and Human Disease pp 40–103Edited by Syrjänen K., Gissmann L., Koss L. G. Berlin: Springer-Verlag;
    [Google Scholar]
  17. Sundberg J. P., Van Ranst M., Burk R. D., Jenson A. B. 1997; The nonhuman (animal) papillomaviruses: host range, epitope conservation, and molecular diversity. In Human Papillomavirus Infections in Dermatovenereology pp 47–68Edited by Gross G., von Krogh G. Boca Raton: CRC Press;
    [Google Scholar]
  18. Sundberg J. P., Van Ranst M., Jenson A. B. 2001; Papillomavirus infections. In Infectious Diseases of Wild Mammals . , 3rd edn. pp 223–231Edited by Williams E. S., Barker I. K. Ames: Iowa State University Press;
  19. Tachezy R., Rector A., Havelkova M., Wollants E., Fiten P., Opdenakker G., Jenson B., Sundberg J., Van Ranst M. 2002; Avian papillomaviruses: the parrot Psittacus erithacus papillomavirus (PePV) genome has a unique organization of the early protein region and is phylogenetically related to the chaffinch papillomavirus. BMC Microbiol 2:19–27 [CrossRef]
    [Google Scholar]
  20. Terai M., Burk R. D. 2002; Identification and characterization of 3 novel genital human papillomaviruses by overlapping polymerase chain reaction: cand HPV89, cand HPV90, and cand HPV91. J Infect Dis 185:1794–1797 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  22. Van Ranst M., Kaplan J. B., Burk R. D. 1992; Phylogenetic classification of human papillomaviruses: correlation with clinical manifestations. J Gen Virol 73:2653–2660 [CrossRef]
    [Google Scholar]
  23. Van Ranst M., Kaplan J. B., Sundberg J. P., Burk R. D. 1995; Molecular evolution of papillomaviruses. In Molecular Basis of Virus Evolution pp 455–476Edited by Gibbs A., Calisher C. H., Garcia-Arenal. Cambridge: Cambridge University Press;
    [Google Scholar]
  24. Xia X., Xie Z. 2001; dambe: software package for data analysis in molecular biology and evolution. J Hered 92:371–373 [CrossRef]
    [Google Scholar]
  25. Zardoya R., Meyer A. 2001; Vertebrate phylogeny: limits of inference of mitochondrial genome and nuclear rDNA data due to an adverse phylogenetic signal/noise ratio. In Major Events in Early Vertebrate Evolution pp 135–155Edited by Ahlberg P. F. London: Taylor & Francis;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/003186-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/003186-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed