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Journal of General Virology header logo Journal of General Virology

Volume 89, Issue 12

Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding EF1 No Access

Abstract

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (EF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and EF1.

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2008-12-01
2025-02-15
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/content/journal/jgv/10.1099/vir.0.2008/003103-0
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Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1
J. Gen. Virol. 89, 2998 (2008); https://doi.org/10.1099/vir.0.2008/003103-0
/content/journal/jgv/10.1099/vir.0.2008/003103-0
/content/journal/jgv/10.1099/vir.0.2008/003103-0
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