1887

Abstract

nucleopolyhedrovirus (AdorNPV) was isolated from overwintering larvae from an orchard in the UK. The nucleotide sequence of the AdorNPV DNA genome was determined and analysed. The genome contains 111724 bp and has a G+C content of 35.0 mol%. The analysis predicted 121 ORFs of 150 nt or larger. Of these putative genes, 118 were homologous to genes identified previously in the nucleopolyhedrovirus (AdhoNPV) genome (83.3–100 % aa identity), and three AdorNPV ORFs were unique. There were four small homologous regions that consisted of a similar core sequence and at the same relative positions in the genome as AdhoNPV, but they differed in the number of repeats and orientation. Some genes that have been reported to have major roles in baculovirus biology were either absent or truncated in the AdorNPV genome. These included chitinase, which is involved in the liquefaction of the host, and the C-terminal of the ecdysteroid UDP-glucosyltransferase () protein, which was truncated by 149 aa compared with AdhoNPV, with essential amino acids absent. The AdorNPV genome encoded two inhibitor of apoptosis () genes compared with three in AdhoNPV and three genes compared with four in AdhoNPV. The susceptibility of larvae to AdorNPV was evaluated in laboratory bioassays using inoculation by microdroplet feeding and applied dose assays. LD for neonates was 56 occlusion bodies rising to 2.3×10 for fifth instar larvae. Median survival time values using an LD dose were 8.8 days for neonates and 7.0 days for fifth instar larvae.

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2008-11-01
2024-12-05
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References

  1. Afonso C. L., Tulman E. R., Lu Z., Balinsky C. A., Moser B. A., Becnel J. J., Rock D. L., Kutish G. F. 2001; Genome sequence of a baculovirus pathogenic for Culex nigripalpus . J Virol 75:11157–11165 [CrossRef]
    [Google Scholar]
  2. Ahrens C. H., Russell R. L. Q., Funk C. J., Evans J. T., Harwood S. H., Rohrmann G. F. 1997; The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229:381–399 [CrossRef]
    [Google Scholar]
  3. Allaway G. P., Payne C. C. 1984; Host range and virulence of five baculoviruses from lepidopterous hosts. Ann Appl Biol 105:29–37 [CrossRef]
    [Google Scholar]
  4. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605 [CrossRef]
    [Google Scholar]
  5. Bideshi D. K., Renault S., Stasiak K. 2003; Phylogenetic analysis and possible function of bro -like genes, a multigene family widespread among large double-stranded DNA viruses of invertebrates and bacteria. J Gen Virol 84:2531–2544 [CrossRef]
    [Google Scholar]
  6. Birnbaum M. J., Clem R. J., Miller L. K. 1994; An apoptosis-inhibiting gene from a nuclear polyhedrosis virus encoding a polypeptide with Cys/His sequence motifs. J Virol 68:2521–2528
    [Google Scholar]
  7. Bliss C. I. 1937; The calculation of the time mortality curve. Ann Appl Biol 24:815–852
    [Google Scholar]
  8. Broer R., Heldens J. G. M., van Strien E. A., Zuidema D., Vlak J. M. 1998; Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication. J Gen Virol 79:1563–1572
    [Google Scholar]
  9. Bulach D. M., Kumar C. A., Zaia A., Liang B. F., Tribe D. E. 1999; Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invertebr Pathol 73:59–73 [CrossRef]
    [Google Scholar]
  10. Carstens E. B., Wu Y. 2007; No single homologous repeat region is essential for DNA replication of the baculovirus Autographa californica multiple nucleopolyhedrovirus. J Gen Virol 88:114–122 [CrossRef]
    [Google Scholar]
  11. Chen X., Sun X., Hu Z., Li M., O'Reilly D. R., Zuidema D., Vlak J. M. 2000; Genetic engineering of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus as an improved pesticide. J Invertebr Pathol 76:140–146 [CrossRef]
    [Google Scholar]
  12. Crook N. E., Payne C. C. 1980; Comparison of three methods of ELISA for baculoviruses. J Gen Virol 46:29–37 [CrossRef]
    [Google Scholar]
  13. Crook N. E., Clem R. J., Miller L. K. 1993; An apoptosis-inhibiting baculovirus gene with a zinc-finger like motif. J Virol 67:2168–2174
    [Google Scholar]
  14. Cross J. V., Solomon M. G., Chandler D., Jarrett P., Richardson P. N., Winstanley D., Bathon H., Huber J., Keller B. other authors 1999; Biocontrol of pests of apples and pears in northern and central Europe: 1. Microbial agents and nematodes. Biocontrol Sci Technol 9:125–149 [CrossRef]
    [Google Scholar]
  15. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395 [CrossRef]
    [Google Scholar]
  16. Duffy S. P., Young A. M., Morin B., Lucarotti C. J., Koop B. F., Levin D. B. 2006; Sequence analysis and organization of the Neodiprion abietis nucleopolyhedrovirus genome. J Virol 80:6952–6963 [CrossRef]
    [Google Scholar]
  17. Eldridge R., O'Reilly D. R., Hammock B. D., Miller L. K. 1992; Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene. Appl Environ Microbiol 58:1583–1591
    [Google Scholar]
  18. Flipsen J. T., Mans R. M., Kleefsman A. W., Knebel-Morsdorf D., Vlak J. M. 1995; Deletion of the baculovirus ecdysteroid UDP glucosyltransferase gene induces early degeneration of Malpighian tubules in infected insects. J Virol 69:4529–4532
    [Google Scholar]
  19. Friesen P. D. 1997; Regulation of baculovirus early gene expression. In The Baculoviruses pp 141–170Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  20. Garcia-Maruniak A., Maruniak J. E., Zanotto P. M. A., Doumbouya A. E., Liu J. C., Merritt T. A., Lanoie J. S. 2004; Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus. J Virol 78:7036–7051 [CrossRef]
    [Google Scholar]
  21. Gomi S., Zhou C. E., Yih W. Y., Majima K., Maeda S. 1997; Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. Virology 230:35–47 [CrossRef]
    [Google Scholar]
  22. Gomi S., Majima K., Maeda S. 1999; Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80:1323–1337
    [Google Scholar]
  23. Guarino L. A., Summers M. D. 1986; Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression. J Virol 60:215–223
    [Google Scholar]
  24. Guarino L. A., Gonzalez M. A., Summers M. D. 1986; Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. J Virol 60:224–229
    [Google Scholar]
  25. Guennelon G., Audemard H., Fremond J. C., El Idrissi Ammari M. A. 1981; Progrès réalisés dans l'elevage permanent du Carpocapse ( Laspeyresia pomonella L.) sur milieu artificiel. Agronomie 1:59–64 [CrossRef]
    [Google Scholar]
  26. Habib S., Hasnain S. E. 2000; Differential activity of two non-hr origins during replication of the baculovirus Autographa californica nuclear polyhedrosis virus genome. J Virol 74:5182–5189 [CrossRef]
    [Google Scholar]
  27. Hawtin R. E., Zarkowska T., Arnold K., Thomas C. J., Gooday G. W., King L. A., Kuzio J. A., Possee R. D. 1997; Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology 238:243–253 [CrossRef]
    [Google Scholar]
  28. Hayakawa T., Ko R., Okano K., Seong S.-I., Goto C., Maeda S. 1999; Sequence analysis of the Xestia c-nigrum granulovirus genome. Virology 262:277–297 [CrossRef]
    [Google Scholar]
  29. Heldens J. G., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1997; Identification and functional analysis of a non-hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus. J Gen Virol 78:1497–1506
    [Google Scholar]
  30. Hilton S., Winstanley D. 2007; Identification and functional analysis of the origins of DNA replication in the Cydia pomonella granulovirus genome. J Gen Virol 88:1496–1504 [CrossRef]
    [Google Scholar]
  31. Hilton S., Winstanley D. 2008; Biological characterization of an English granulovirus from the summer fruit tortrix moth, Adoxophyes orana . J Invertebr Pathol 97:298–305 [CrossRef]
    [Google Scholar]
  32. Hu Z. H., Broer R., Westerlaken J. 1997; Characterization of the ecdysteroid UDP-glucosyltransferase gene of a single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria . Virus Res 47:91–97 [CrossRef]
    [Google Scholar]
  33. Hughes P. R., Wood H. A. 1981; A synchronous peroral technique for the bioassay of insect viruses. J Invertebr Pathol 37:154–159 [CrossRef]
    [Google Scholar]
  34. Hughes P. R., van Beek N. A. M., Wood H. A. 1986; A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctuid larvae. J Invertebr Pathol 48:187–192 [CrossRef]
    [Google Scholar]
  35. Hyink O., Dellow R. A., Olsen M. J., Caradoc-Davies K. M. B., Drake K., Herniou E. A., Cory J. S., O'Reilly D. R., Ward V. K. 2002; Whole genome analysis of the Epiphyas postvittana nucleopolyhedrovirus. J Gen Virol 83:957–971
    [Google Scholar]
  36. IJkel W. F. J., van Strien E. A., Heldens J. G. M., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1999; Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J Gen Virol 80:3289–3304
    [Google Scholar]
  37. IJkel W. F. J., Westenberg M., Goldbach R. W., Blissard G. W., Vlak J. M. 2000; A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275:30–41 [CrossRef]
    [Google Scholar]
  38. Ishii T., Nakai M., Okuno S., Takatsuka J., Kunimi Y. 2003; Characterization of Adoxophyes honmai single-nucleocapsid nucleopolyhedrovirus: morphology, structure, and effects on larvae. J Invertebr Pathol 83:206–214 [CrossRef]
    [Google Scholar]
  39. Jehle J. A., Lange M., Wang H. L., Hu Z. H., Wang Y. J., Hauschild W. 2006; Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology 346:180–193 [CrossRef]
    [Google Scholar]
  40. Kool M., Goldbach R. W., Vlak J. M. 1994; A putative non-hr origin of DNA replication in the Hin dIII-K fragment of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus. J Gen Virol 75:3345–3352 [CrossRef]
    [Google Scholar]
  41. Kool M., Ahrens C. H., Vlak J. M., Rohrmann G. F. 1995; Replication of baculovirus DNA. J Gen Virol 76:2103–2118 [CrossRef]
    [Google Scholar]
  42. Kuzio J., Pearson M. N., Harwood S. H., Funk C. J., Evans J. T., Slavicek J. M., Rohrmann G. F. 1999; Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar . Virology 253:17–34 [CrossRef]
    [Google Scholar]
  43. Lange M., Jehle J. A. 2003; The genome of Cryptophlebia leucotreta granulovirus. Virology 317:220–236 [CrossRef]
    [Google Scholar]
  44. Lauzon H. A. M., Lucarotti C. J., Krell P. J., Feng Q. L., Retnakaran A., Arif B. M. 2004; Sequence and organization of the Neodiprion lecontei nucleopolyhedrovirus genome. J Virol 78:7023–7035 [CrossRef]
    [Google Scholar]
  45. Li L., Donly C., Li Q., Willis L. G., Keddie B. A., Erlandson M. A., Theilmann D. A. 2002; Identification and genomic analysis of a second species of nucleopolyhedrovirus isolated from Mamestra configurata . Virology 297:226–244 [CrossRef]
    [Google Scholar]
  46. Li L. L., Li O. J., Willis L. G., Erlandson M., Theilmann D. A., Donly C. 2005; Complete comparative genomic analysis of two field isolates of Mamestra configurata nucleopolyhedrovirus-A. J Gen Virol 86:91–105 [CrossRef]
    [Google Scholar]
  47. Li Q. J., Donly C., Li L. L., Willis L. G., Theilmann D. A., Erlandson M. 2002; Sequence and organization of the Mamestra configurata nucleopolyhedrovirus genome. Virology 294:106–121 [CrossRef]
    [Google Scholar]
  48. Lu A., Miller L. K. 1997; Regulation of baculovirus late and very late gene expression. In The Baculoviruses pp 193–216Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  49. Lu A., Krell P., Vlak J., Rohrmann G. 1997; Baculovirus DNA replication. In The Baculoviruses pp 171–192Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  50. Luque T., Finch R., Crook N., O'Reilly D. R., Winstanley D. 2001; The complete sequence of Cydia pomonella granulovirus genome. J Gen Virol 82:2531–2547
    [Google Scholar]
  51. Monsma S. A., Oomens A. G. P., Blissard G. W. 1996; The GP64 envelope fusion protein is an essential baculovirus protein required for cell to cell transmission of infection. J Virol 70:4607–4616
    [Google Scholar]
  52. Morris T. D., Todd J. W., Fisher B., Miller L. K. 1994; Identification of lef-7: a baculovirus gene affecting late gene expression. Virology 200:360–369
    [Google Scholar]
  53. Nakai M., Goto C., Kang W. K., Shikata M., Luque T., Kunimi Y. 2003; Genome sequence and organization of a nucleopolyhedrovirus isolated from the smaller tea tortrix, Adoxophyes honmai . Virology 316:171–183 [CrossRef]
    [Google Scholar]
  54. O'Reilly D. R. 1995; Baculovirus-encoded ecdysteroid UDP-glucosyltransferases. Insect Biochem Mol Biol 25:541–550 [CrossRef]
    [Google Scholar]
  55. O'Reilly D. 1997; Auxiliary genes of baculoviruses. In The Baculoviruses pp 267–300Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  56. O'Reilly D. R., Miller L. K. 1991; Improvement of a baculovirus pesticide by deletion of the EGT gene. Biotechnol 9:1086–1089 [CrossRef]
    [Google Scholar]
  57. O'Reilly D. R., Howarth O. W., Rees H. H., Miller L. K. 1991; Structure of the ecdysone glucoside formed by a baculovirus ecdysteroid UDP-glucosyltransferase. Insect Biochem 21:795–801 [CrossRef]
    [Google Scholar]
  58. O'Reilly D. R., Hails R. S., Kelly T. J. 1998; The impact of host developmental status on baculovirus replication. J Invertebr Pathol 72:269–275 [CrossRef]
    [Google Scholar]
  59. Pang Y., Yu J. X., Wang L. H., Hu X. H., Bao W. D., Li G., Chen C., Han H., Hu S. N., Yang H. M. 2001; Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology 287:391–404 [CrossRef]
    [Google Scholar]
  60. Pearson M. N., Groten C., Rohrmann G. F. 2000; Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae . J Virol 74:6126–6131 [CrossRef]
    [Google Scholar]
  61. Pearson M. N., Russell R. L. Q., Rohrmann G. F. 2001; Characterization of a baculovirus-encoded protein that is associated with infected-cell membranes and budded virions. Virology 291:22–31 [CrossRef]
    [Google Scholar]
  62. Rapp J. C., Wilson J. A., Miller L. K. 1998; Nineteen baculovirus open reading frames, including LEF-12, support late gene expression. J Virol 72:10197–10206
    [Google Scholar]
  63. Sharp P. M., Lloyd A. T. 1993; Regional base composition variation along yeast chromosome III: evolution of chromosome primary structure. Nucleic Acids Res 21:179–183 [CrossRef]
    [Google Scholar]
  64. Slack J. M., Kuzio J., Faulkner P. 1995; Characterization of v-cath, a cathepsin l-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus. J Gen Virol 76:1091–1098 [CrossRef]
    [Google Scholar]
  65. Slavicek J. M., Popham H. J. R., Riegel C. I. 1999; Deletion of the Lymantria dispar multicapsid nucleopolyhedrovirus ecdysteroid UDP-glucosyl transferase gene enhances viral killing speed in the last instar of the gypsy moth. Biol Control 16:91–103 [CrossRef]
    [Google Scholar]
  66. Smith I. R. L., Crook N. E. 1988a; In vivo isolation of baculovirus genotypes. Virology 166:240–244 [CrossRef]
    [Google Scholar]
  67. Smith I. R. L., Crook N. E. 1988b; Physical maps of the genomes of four variants of Artogeia rapae granulosis virus. J Gen Virol 69:1741–1747 [CrossRef]
    [Google Scholar]
  68. Theilmann D. A., Blissard G. W., Bonning B., Jehle J., O'Reilly D. R., Rohrmann G. F., Theim S., Vlak J. M. 2005; Baculoviridae . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp 177–185Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
    [Google Scholar]
  69. Tournas V. 1994; Heat-resistant fungi of importance to the food and beverage industry. Crit Rev Microbiol 20:243–263 [CrossRef]
    [Google Scholar]
  70. Treacy M. F., All J. N., Ghidiu G. M. 1997; Effect of ecdysteroid UDP-glucosyltransferase gene deletion on efficacy of a baculovirus against Heliothis virescens and Trichoplusia ni (Lepidoptera: Noctuidae). J Econ Entomol 90:1207–1214 [CrossRef]
    [Google Scholar]
  71. Vucic D., Kaiser W. J., Harvey A. J., Miller L. K. 1997; Inhibition of reaper-induced apoptosis by interaction with inhibitor of apoptosis proteins (IAPs). Proc Natl Acad Sci U S A 94:10183–10188 [CrossRef]
    [Google Scholar]
  72. Wormleaton S. L., Winstanley D. 2001; Phylogenetic analysis of conserved genes within the ecdysteroid UDP-glucosyltransferase gene region of the slow-killing Adoxophyes orana granulovirus. J Gen Virol 82:2295–2305
    [Google Scholar]
  73. Wormleaton S., Kuzio J., Winstanley D. 2003; The complete sequence of the Adoxophyes orana granulovirus genome. Virology 311:350–365 [CrossRef]
    [Google Scholar]
  74. Zanotto P. M. D., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses – evolutionary rates and host associations. J Invertebr Pathol 62:147–164 [CrossRef]
    [Google Scholar]
  75. Zemskov E. A., Kang W., Maeda S. 2000; Evidence for nucleic acid binding ability and nucleosome association of Bombyx mori nucleopolyhedrovirus BRO proteins. J Virol 74:6784–6789 [CrossRef]
    [Google Scholar]
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