1887

Abstract

A combined functional investigation on the four infectivity factors (PIFs) of single nucleocapsid nucleopolyhedrovirus (HearNPV) was conducted in this study. HearNPV bacmids with deletions of (), (), () and () were constructed individually by homologous recombination in cells. Repaired bacmids with respective s were also constructed. Western blot analyses revealed that all four PIFs were structural components of the envelope of HearNPV occlusion-derived virus (ODV). Electron microscopy showed that deletion of the s did not have any obvious effects on the morphology of the occlusion bodies (OBs). Bioassay analyses indicated that deletion of any of the above s resulted in loss of oral infectivity of OBs. The mixtures of the four -deletion mutants also resulted in deficiency of oral infectivity, implying that the four PIFs must be structural components of the same ODV to accomplish their function. Repairing of the respective genes into the -deletion bacmids could rescue the oral infectivity of the -deletion viruses. Calcofluor, which can damage the peritrophic membrane (PM), could not rescue the defects of the oral infectivity of the -deletion viruses, indicating that the PM is not likely to be the functional target of the PIFs.

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2008-09-01
2019-11-19
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