1887

Abstract

The genetic diversity of Puumala hantavirus (PUUV) was studied in a local population of its natural host, the bank vole (). The trapping area (2.5×2.5 km) at Konnevesi, Central Finland, included 14 trapping sites, at least 500 m apart; altogether, 147 voles were captured during May and October 2005. Partial sequences of the S, M and L viral genome segments were recovered from 40 animals. Seven, 12 and 17 variants were detected for the S, M and L sequences, respectively; these represent new wild-type PUUV strains that belong to the Finnish genetic lineage. The genetic diversity of PUUV strains from Konnevesi was 0.2–4.9 % for the S segment, 0.2–4.8 % for the M segment and 0.2–9.7 % for the L segment. Most nucleotide substitutions were synonymous and most deduced amino acid substitutions were conservative, probably due to strong stabilizing selection operating at the protein level. Based on both sequence markers and phylogenetic clustering, the S, M and L sequences could be assigned to two groups, ‘A’ and ‘B’. Notably, not all bank voles carried S, M and L sequences belonging to the same group, i.e. SML or SML. A substantial proportion (8/40, 20 %) of the newly characterized PUUV strains possessed reassortant genomes such as SML, SML or SML. These results suggest that at least some of the PUUV reassortants are viable and can survive in the presence of their parental strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/001248-0
2008-07-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/7/1649.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/001248-0&mimeType=html&fmt=ahah

References

  1. Asikainen, K., Hänninen, T., Henttonen, H., Niemimaa, J., Laakkonen, J., Andersen, H. K., Bille, N., Leirs, H., Vaheri, A. & Plyusnin, A. ( 2000; ). Molecular evolution of Puumala hantavirus in Fennoscandia: phylogenetic analysis of strains from two recolonization routes, Karelia and Denmark. J Gen Virol 81, 2833–2841.
    [Google Scholar]
  2. Bernshtein, A. D., Apekina, N. S., Mikhailova, T. V., Myasnikov, Yu. A., Khlyap, L. A., Korotkov, Yu. S. & Gavrilovskaya, I. N. ( 1999; ). Dynamics of Puumala hantavirus infection in naturally infected bank voles (Clethrionomys glareolus). Arch Virol 144, 2415–2428.[CrossRef]
    [Google Scholar]
  3. Brummer-Korvenkontio, M., Vaheri, A., Hovi, T., von Bonsdorff, C.-H., Vuorimies, J., Manni, T., Penttinen, K., Oker-Blom, N. & Lähdevirta, J. ( 1980; ). Nephropathia epidemica: detection of antigen in bank vole and serological diagnosis of human infection. J Infect Dis 141, 131–134.[CrossRef]
    [Google Scholar]
  4. Brummer-Korvenkontio, M., Henttonen, H. & Vaheri, A. ( 1982; ). Hemorrhagic fever with renal syndrome in Finland: ecology and virology of nephropathia epidemica. Scand J Infect Dis Suppl 36, 131–134.
    [Google Scholar]
  5. Chare, E. R., Gould, E. A. & Holmes, E. C. ( 2003; ). Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses. J Gen Virol 84, 2691–2730.[CrossRef]
    [Google Scholar]
  6. Crawley, M. C. ( 1969; ). Movements and home-ranges of Clethrionomys glareolus Schreber and Apodemus sylvaticus L. in north-east England. Oikos 20, 310–319.[CrossRef]
    [Google Scholar]
  7. Deyde, V. M., Khristova, M. L., Rollin, P. E., Ksiazek, T. G. & Nichol, S. ( 2006; ). Crimean-Congo hemorraghic fever virus genomics and global diversity. J Virol 80, 8834–8842.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), 3.66 version. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, WA, USA.
  9. Gavrilovskaya, I. N., Apekina, N. S., Bernshtein, A. D., Demia, V. T., Okulova, N. M., Myasnikov, Y. A. & Chumanov, M. P. ( 1990; ). Pathogenesis of hemorrhagic fever with renal syndrome virus infection and mode of horizontal transmission of hantavirus in bank voles. Arch Virol Suppl 1, 57–62.
    [Google Scholar]
  10. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  11. Hamano, T., Matsuo, K., Hibi, Y., Victoriano, A. F., Takahashi, N., Mabuchi, Y., Soji, T., Irie, S., Sawanpanyalert, P. & other authors ( 2007; ). A single-nucleotide synonymous mutation in the gag gene controlling human immunodeficiency virus type 1 virion production. J Virol 81, 1528–1533.[CrossRef]
    [Google Scholar]
  12. Henderson, W. W., Monroe, M. C., St. Jeor, S. C., Thayer, W. P., Rowe, J. E., Peters, C. J. & Nichol, S. T. ( 1995; ). Naturally occurring Sin Nombre virus genetic reassortants. Virology 214, 602–610.[CrossRef]
    [Google Scholar]
  13. Hörling, J., Lundkvist, Å., Jaarola, M., Plyusnin, A., Tegelström, H., Persson, K., Lehväslaiho, H., Hörnfeldt, B., Vaheri, A. & Niklasson, B. ( 1996; ). Distribution and genetic heterogeneity of Puumala virus in Sweden. J Gen Virol 77, 2555–2562.[CrossRef]
    [Google Scholar]
  14. Huelsenbeck, J. P. & Ronquist, F. ( 2001; ). mrbayes: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.[CrossRef]
    [Google Scholar]
  15. Kallio, E. R., Klingström, J., Gustafsson, E., Manni, T., Vaheri, A., Henttonen, H., Vapalahti, O. & Lundkvist, Å. ( 2006a; ). Prolonged survival of Puumala hantavirus outside the host: evidence for indirect transmission via the environment. J Gen Virol 87, 2127–2134.[CrossRef]
    [Google Scholar]
  16. Kallio, E. R., Poikonen, A., Vaheri, A., Vapalahti, O., Henttonen, H., Koskela, E. & Mappes, T. ( 2006b; ). Maternal antibodies postpone hantavirus infection and enhance individual breeding success. Proc Biol Sci 273, 2771–2776.[CrossRef]
    [Google Scholar]
  17. Kallio, E. R., Voutilainen, L., Vapalahti, O., Vaheri, A., Henttonen, H., Koskela, E. & Mappes, T. ( 2007; ). Endemic hantavirus infection impairs the winter survival of its rodent host. Ecology 88, 1911–1916.[CrossRef]
    [Google Scholar]
  18. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  19. Li, D., Schmaljohn, A. L., Anderson, K. & Schmaljohn, C. S. ( 1995; ). Complete nucleotide sequences of M and S segments of two hantavirus isolates from California: evidence for reassortment in nature among viruses related to hantavirus pulmonary syndrome. Virology 206, 973–983.[CrossRef]
    [Google Scholar]
  20. Meyer, B. J. & Schmaljohn, C. S. ( 2000; ). Persistent hantavirus infections: characteristics and mechanisms. Trends Microbiol 8, 61–67.[CrossRef]
    [Google Scholar]
  21. Mitchell-Jones, A. J., Amori, G., Bogdanowicz, W., Krystufek, B., Reijnders, P. J. H., Spitzenberger, F., Stubbe, M., Thissen, J. B. M., Vohralik, V. V. & Zima, J. ( 1999; ). Clethrionomys glareolus. In The Atlas of European Mammals, pp. 212–213. Edited by A. J. Mitchell-Jones. London: T & AD Poyser Natural History.
  22. Nichol, S., Beaty, B. J., Elliott, R. M., Goldbach, R., Plyusnin, A., Schmaljohn, C. S. & Tesh, R. B. ( 2005; ). Bunyaviridae. In Virus Taxonomy. Eighth report of the International Committee on Taxonomy of Viruses, pp. 695–716. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. Amsterdam: Elsevier Academic Press.
  23. Niklasson, B., Hörnfeldt, B., Lundkvist, Å., Björsten, S. & Leduc, J. ( 1995; ). Temporal dynamics of Puumala virus antibody prevalence in voles and of nephropathia epidemica incidence in humans. Am J Trop Med Hyg 53, 134–140.
    [Google Scholar]
  24. Novella, I. S., Zarate, S., Metzgar, D. & Ebendick-Corpus, B. E. ( 2004; ). Positive selection of synonymous mutations in vesicular stomatitis virus. J Mol Biol 342, 1415–1421.[CrossRef]
    [Google Scholar]
  25. Olsson, G. E., White, N., Ahlm, C., Elgh, F., Verlemyr, A. C., Juto, P. & Palo, P. T. ( 2002; ). Demographic factors associated with hantavirus infection in bank voles (Clethrionomys glareolus). Emerg Infect Dis 8, 924–929.[CrossRef]
    [Google Scholar]
  26. Plyusnin, A. ( 2002; ). Genetics of hantaviruses: implications to taxonomy. Arch Virol 147, 665–682.[CrossRef]
    [Google Scholar]
  27. Plyusnin, A. & Morzunov, S. P. ( 2001; ). Virus evolution and genetic diversity of hantaviruses and their rodent hosts. Curr Top Microbiol Immunol 256, 47–75.
    [Google Scholar]
  28. Plyusnin, A., Vapalahti, O., Lehväslaiho, H., Apekina, N., Mikhailova, T., Gavrilovskaya, I., Laakkonen, J., Niemimaa, J., Henttonen, H. & other authors ( 1995a; ). Genetic variation of wild Puumala viruses within the serotype, local rodent populations and individual animal. Virus Res 38, 25–41.[CrossRef]
    [Google Scholar]
  29. Plyusnin, A., Cheng, Y., Vapalahti, O., Pejcoch, M., Unar, J., Jelinkova, Z., Lehväslaiho, H., Lundkvist, Å. & Vaheri, A. ( 1995b; ). Genetic variation in Tula hantaviruses: sequence analysis of the S and M segments of strains from Central Europe. Virus Res 39, 237–250.[CrossRef]
    [Google Scholar]
  30. Plyusnin, A., Cheng, Y., Vapalahti, O., Lehväslaiho, H. & Vaheri, A. ( 1996a; ). Quasispecies in wild Tula hantavirus populations. J Virol 70, 9060–9063.
    [Google Scholar]
  31. Plyusnin, A., Vapalahti, O. & Vaheri, A. ( 1996b; ). Hantaviruses: genome structure, expression and evolution. J Gen Virol 77, 2677–2687.[CrossRef]
    [Google Scholar]
  32. Plyusnin, A., Hörling, J., Kanerva, M., Mustonen, J., Cheng, Y., Partanen, J., Vapalahti, O., Kukkonen, S.K., Niemimaa, J. & other authors ( 1997; ). Puumala hantavirus genome in patients with nephropathia epidemica: correlation of PCR positivity with HLA haplotype and link to viral sequences in local rodents. J Clin Microbiol 35, 1090–1096.
    [Google Scholar]
  33. Plyusnina, A., Aberle, S. W., Aberle, J. H. & Plyusnin, A. ( 2006; ). Genetic analysis of Puumala hantavirus strains from Austria. Scand J Infect Dis 38, 512–519.[CrossRef]
    [Google Scholar]
  34. Sall, A. A., Zanotto, P. M. de A., Sene, O. K., Zeller, H. G., Digoutte, J. P., Thiongane, Y. & Bouloy, M. ( 1999; ). Genetic reassortment of Rift Valley fever virus in nature. J Virol 73, 8196–8200.
    [Google Scholar]
  35. Sauvage, F., Langlais, M., Yoccoz, N. G. & Pontier, D. ( 2003; ). Modelling hantavirus in fluctuating populations of bank voles: the role of indirect transmission on virus persistence. J Anim Ecol 72, 1–13.[CrossRef]
    [Google Scholar]
  36. Schmaljohn, C. S. & Dalrymple, J. M. ( 1983; ). Analysis of Hantaan virus RNA: evidence for a new genus of Bunyaviridae. Virology 131, 482–491.[CrossRef]
    [Google Scholar]
  37. Schmaljohn, C. & Hjelle, B. ( 1997; ). Hantaviruses: a global disease problem. Emerg Infect Dis 3, 95–104.[CrossRef]
    [Google Scholar]
  38. Sibold, C., Meisel, H., Kruger, D. H., Labuda, M., Lysy, J., Kozuch, O., Pejcoch, M., Vaheri, A. & Plyusnin, A. ( 1999; ). Recombination in Tula hantavirus evolution: analysis of genetic lineages from Slovakia. J Virol 73, 667–675.
    [Google Scholar]
  39. Sironen, T., Vaheri, A. & Plyusnin, A. ( 2001; ). Molecular evolution of Puumala hantavirus. J Virol 75, 11803–11810.[CrossRef]
    [Google Scholar]
  40. Sironen, T., Kallio, E., Vaheri, A., Lundkvist, Å. & Plyusnin, A. ( 2008; ). Quasispecies and fixation of synonymous mutation in hantavirus transmission. J Gen Virol 89, 1309–1313.[CrossRef]
    [Google Scholar]
  41. Urquidi, V. & Bishop, D. H. L. ( 1992; ). Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses. J Gen Virol 73, 2255–2265.[CrossRef]
    [Google Scholar]
  42. Vapalahti, O., Mustonen, J., Lundkvist, Å., Henttonen, H., Plyusnin, A. & Vaheri, A. ( 2003; ). Hantavirus infections in Europe. Lancet Infect Dis 3, 653–661.[CrossRef]
    [Google Scholar]
  43. Wilson, D. E. & Reeders, D. M. ( 2005; ). Mammal Species of the World. A Taxonomic and Geographic Reference, 3rd edn, vol. 2. Baltimore: The Johns Hopkins University Press.
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/001248-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/001248-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error