1887

Abstract

multicapsid nucleopolyhedrovirus (ThorMNPV) carrying an enhanced green fluorescent protein (EGFP) gene expression cassette (vThGFP) was used to study host-range mechanisms. Infection kinetics showed that vThGFP replication in Sf21 cells was too slow to suppress cell growth. Wide-host-range MNPV (AcMNPV) could speed up vThGFP infection and enhance the vThGFP infection rate in Sf21 cells. The enhancement was not due to recombination, as no recombinant virus was isolated from co-infection by plaque assay. No improvement of vThGFP infection in Sf21 was found by AcMNPV cosmid transactivation assay. However, culture medium from Sf21 cells infected with AcMNPV did enhance vThGFP replication in Sf21. Third-instar larvae of , and were not killed by feeding with vThGFP polyhedra but were killed by intrahaemocoelic injection using budded viruses (BVs). This suggested that insufficient BVs were generated during the primary infection in the midgut. vThGFP infected haemocytes, tracheae and Malpighian tubules but not fat bodies of larvae of , and . Third-instar larvae co-infected by injection with vThGFP and vAcDsRed2, an AcMNPV expressing a red fluorescent protein gene, showed EGFP expression in the fat body. This result suggests that vAcDsRed2 could help vThGFP to replicate in the fat body or -activate EGFP expression in the fat body. All these results suggested that slow cell infection, insufficient primary infection and inability to replicate in the fat body control the host range of ThorMNPV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/000695-0
2008-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/6/1402.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/000695-0&mimeType=html&fmt=ahah

References

  1. Cheng X. W., Carner G. R. 2000; Characterization of a single-nucleocapsid nucleopolyhedrovirus of Thysanoplusia orichalcea L. (Lepidoptera: Noctuidae) from Indonesia. J Invertebr Pathol 75:279–287 [CrossRef]
    [Google Scholar]
  2. Cheng X., Krell P., Arif B. 2001; P34.8 (GP37) is not essential for baculovirus replication. J Gen Virol 82:299–305
    [Google Scholar]
  3. Cheng X. W., Carner G. R., Lange M., Jehle J. A., Arif B. M. 2005; Biological and molecular characterization of a multicapsid nucleopolyhedrovirus from Thysanoplusia orichalcea (L.) (Lepidoptera: Noctuidae). J Invertebr Pathol 88:126–135 [CrossRef]
    [Google Scholar]
  4. Croizier G., Croizier L., Argaud O., Poudevigne D. 1994; Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. Proc Natl Acad Sci U S A 91:48–52 [CrossRef]
    [Google Scholar]
  5. Federici B. A. 1997; Baculovirus pathogenesis. In The Baculoviruses pp 33–59Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  6. Groner A. 1986; Specificity and safety of baculoviruses. In The Biology of Baculoviruses vol 1 pp 177–202Edited by Granados R. R., Federici B. A. Boca Raton, FL: CRC Press;
    [Google Scholar]
  7. Hara K., Funakoshi M., Kawarabata T. 1995; A cloned cell line of Spodoptera exigua has a highly increased susceptibility to the Spodoptera exigua nuclear polyhedrosis virus. Can J Microbiol 41:1111–1116 [CrossRef]
    [Google Scholar]
  8. Kamita S. G., Maeda S. 1997; Sequencing of the putative DNA helicase-encoding gene of the Bombyx mori nuclear polyhedrosis virus and fine-mapping of a region involved in host range expansion. Gene 190:173–179 [CrossRef]
    [Google Scholar]
  9. Katou Y., Ikeda M., Kobayashi M. 2006; Abortive replication of Bombyx mori nucleopolyhedrovirus in Sf9 and High Five cells: defective nuclear transport of the virions. Virology 347:455–465 [CrossRef]
    [Google Scholar]
  10. LeBowitz J. H., McMacken R. 1986; The Escherichia coli dnaB replication protein is a DNA helicase. J Biol Chem 261:4738–4748
    [Google Scholar]
  11. Li L., Harwood S. H., Rohrmann G. F. 1999; Identification of additional genes that influence baculovirus late gene expression. Virology 255:9–19 [CrossRef]
    [Google Scholar]
  12. McDougal V. V., Guarino L. A. 2001; DNA and ATP binding activities of the baculovirus DNA helicase P143. J Virol 75:7206–7209 [CrossRef]
    [Google Scholar]
  13. Miller L. K., Lu A. 1997; The molecular basis of baculovirus host range. In The Baculoviruses pp 217–235Edited by Miller L. K. New York: Plenum Press;
    [Google Scholar]
  14. Moscardi F. 1999; Assessment of the application of baculoviruses for control of Lepidoptera. Annu Rev Entomol 44:257–289 [CrossRef]
    [Google Scholar]
  15. Ogay I. D., Lihoradova O. A., Azimova S. S., Abdukarimov A. A., Slack J., Lynn D. 2006; Transfection of insect cell lines using polyethylenimine. Cytotechnology 51:89–98 [CrossRef]
    [Google Scholar]
  16. O'Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors: a Laboratory Manual New York: W. H. Freeman & Co;
    [Google Scholar]
  17. Palli S. R., Caputo G. F., Sohi S. S., Brownwright A. J., Ladd T. R., Cook B. J., Primavera M., Arif B. M., Retnakaran A. 1996; CfMNPV blocks AcMNPV-induced apoptosis in a continuous midgut cell line. Virology 222:201–213 [CrossRef]
    [Google Scholar]
  18. Pluen A., Netti P. A., Jain R. K., Berk D. A. 1999; Diffusion of macromolecules in agarose gels: comparison of linear and globular configurations. Biophys J 77:542–552 [CrossRef]
    [Google Scholar]
  19. Simon O., Williams T., Lopez-Ferber M., Caballero P. 2004; Virus entry or the primary infection cycle are not the principal determinants of host specificity of Spodoptera spp. nucleopolyhedroviruses. J Gen Virol 85:2845–2855 [CrossRef]
    [Google Scholar]
  20. Thiem S. M., Du X., Quentin M. E., Berner M. M. 1996; Identification of baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. J Virol 70:2221–2229
    [Google Scholar]
  21. Yanase T., Yasunaga C., Hara T., Kawarabata T. 1998; Coinfection of Spodoptera exigua and Spodoptera frugiperda cell lines with the nuclear polyhedrosis viruses of Autographa californica and Spodoptera exigua . Intervirology 41:244–252 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/000695-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/000695-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error