1887

Abstract

The gene of encodes a four-pass transmembrane protein that is required for efficient multiplication of a tobamovirus, TMV-Cg. In this study, the involvement of three homologues in tobamovirus multiplication in was examined. T-DNA insertion mutations in the three homologues, separately or in combination, did not affect TMV-Cg multiplication, whereas, in the genetic background, some combinations reduced it. This result suggests that the homologues are functional in enhancing TMV-Cg multiplication, but their contribution is much less than . Interestingly, the multiplication of another tobamovirus, , was not drastically affected by any combinations of the mutations in and its homologues as far as we examined.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.2008/000539-0
2008-06-01
2019-11-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/89/6/1519.html?itemId=/content/journal/jgv/10.1099/vir.0.2008/000539-0&mimeType=html&fmt=ahah

References

  1. Ahlquist, P., Noueiry, A. O., Lee, W.-M., Kushner, D. B. & Dye, B. T. ( 2003; ). Host factors in positive-strand RNA virus genome replication. J Virol 77, 8181–8186.[CrossRef]
    [Google Scholar]
  2. Asano, M., Satoh, R., Mochizuki, A., Tsuda, S., Yamanaka, T., Nishiguchi, M., Hirai, K., Meshi, T., Naito, S. & Ishikawa, M. ( 2005; ). Tobamovirus-resistant tobacco generated by RNA interference directed against host genes. FEBS Lett 579, 4479–4484.[CrossRef]
    [Google Scholar]
  3. Buck, K. W. ( 1999; ). Replication of tobacco mosaic virus RNA. Philos Trans R Soc Lond B Biol Sci 354, 613–627.[CrossRef]
    [Google Scholar]
  4. Fujisaki, K., Hagihara, F., Azukawa, Y., Kaido, M., Okuno, T. & Mise, K. ( 2004; ). Identification and characterization of the SSB1 locus involved in symptom development by Spring beauty latent virus infection in Arabidopsis thaliana. Mol Plant Microbe Interact 17, 967–975.[CrossRef]
    [Google Scholar]
  5. Fujisaki, K., Ravelo, G. B., Naito, S. & Ishikawa, M. ( 2006; ). Involvement of THH1, an Arabidopsis thaliana homologue of the TOM1 gene, in tobamovirus multiplication. J Gen Virol 87, 2397–2401.[CrossRef]
    [Google Scholar]
  6. Gibbs, A. ( 1999; ). Evolution and origins of tobamoviruses. Philos Trans R Soc Lond B Biol Sci 354, 593–602.[CrossRef]
    [Google Scholar]
  7. Hagiwara, Y., Komoda, K., Yamanaka, T., Tamai, A., Meshi, T., Funada, R., Tsuchiya, T., Naito, S. & Ishikawa, M. ( 2003; ). Subcellular localization of host and viral proteins associated with tobamovirus RNA replication. EMBO J 22, 344–353.[CrossRef]
    [Google Scholar]
  8. Hirokawa, T., Boon-Chieng, S. & Mitaku, S. ( 1998; ). SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14, 378–379.[CrossRef]
    [Google Scholar]
  9. Ishikawa, M. & Okada, Y. ( 2004; ). Replication of tobamovirus RNA. Proc Jpn Acad Ser B Phys Biol Sci 80, 215–224.[CrossRef]
    [Google Scholar]
  10. Kubota, K., Tsuda, S., Tamai, A. & Meshi, T. ( 2003; ). Tomato mosaic virus replication protein suppresses virus-targeted posttranscriptional gene silencing. J Virol 77, 11016–11026.[CrossRef]
    [Google Scholar]
  11. Lartey, R. T., Voss, T. C. & Melcher, U. ( 1996; ). Tobamovirus evolution: gene overlaps, recombination, and taxonomic implications. Mol Biol Evol 13, 1327–1338.[CrossRef]
    [Google Scholar]
  12. Li, Z. Y., Uyeda, I. & Shikata, E. ( 1983; ). Crucifer strain of tobacco mosaic virus isolated from garlic. Mem Fac Agric Hokkaido Univ 13, 542–549.
    [Google Scholar]
  13. Nishikiori, M., Dohi, K., Mori, M., Meshi, T., Naito, S. & Ishikawa, M. ( 2006; ). Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound. J Virol 80, 8459–8468.[CrossRef]
    [Google Scholar]
  14. Ohshima, K., Taniyama, T., Yamanaka, T., Ishikawa, M. & Naito, S. ( 1998; ). Isolation of a mutant of Arabidopsis thaliana carrying two simultaneous mutations affecting tobacco mosaic virus multiplication within a single cell. Virology 243, 472–481.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  16. Tsujimoto, Y., Numaga, T., Ohshima, K., Yano, M. A., Ohsawa, R., Goto, D. B., Naito, S. & Ishikawa, M. ( 2003; ). Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a transmembrane protein that interacts with TOM1. EMBO J 22, 335–343.[CrossRef]
    [Google Scholar]
  17. Yamanaka, T., Komatani, H., Meshi, T., Naito, S., Ishikawa, M. & Ohno, T. ( 1998; ). Complete nucleotide sequence of the genomic RNA of tobacco mosaic virus strain Cg. Virus Genes 16, 173–176.[CrossRef]
    [Google Scholar]
  18. Yamanaka, T., Ohta, T., Takahashi, M., Meshi, T., Schmidt, R., Dean, C., Naito, S. & Ishikawa, M. ( 2000; ). TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein. Proc Natl Acad Sci U S A 97, 10107–10112.[CrossRef]
    [Google Scholar]
  19. Yamanaka, T., Imai, T., Satoh, R., Kawashima, A., Takahashi, M., Tomita, K., Kubota, K., Meshi, T., Naito, S. & Ishikawa, M. ( 2002; ). Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes. J Virol 76, 2491–2497.[CrossRef]
    [Google Scholar]
  20. Yoshii, M., Yoshioka, N., Ishikawa, M. & Naito, S. ( 1998; ). Isolation of an Arabidopsis thaliana mutant in which accumulation of cucumber mosaic virus coat protein is delayed. Plant J 13, 211–219.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.2008/000539-0
Loading
/content/journal/jgv/10.1099/vir.0.2008/000539-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error