1887

Abstract

To gain further insight into the molecular epidemiology of Hantaan virus (HTNV) in Guizhou, China, rodents were captured in this region in 2004 and 2005. In addition, serum samples were collected from four patients. Ten hantaviruses were isolated successfully in cell culture from four humans, two , three and one . The nucleotide sequences for their small (S), medium (M) and partial large (L) segments were determined. Phylogenetic analysis of the S and M segment sequences revealed that all of these isolates belong to the species HTNV, suggesting a spillover of HTNV from to rats. All available isolates from Guizhou were divided into four distinct groups either in the S segment tree or in the M segment tree. The clustering pattern of these isolates in the S segment tree was not in agreement with that in the M or L segment tree, showing that genetic reassortment between HTNV had occurred naturally. Analysis of the S segment sequences from available HTNV strains indicated that they formed three clades. The first clade, which comprised only viruses from Guizhou, was the outgroup of clades II and III. The viruses in the second clade were found in Guizhou and mainly in the far-east Asian region, including China. However, the viruses in the third clade were found in most areas of China, including Guizhou, in which haemorrhagic fever with renal syndrome (HFRS) is endemic. Our results reveal that the highest genetic diversity of HTNV is in a limited geographical region of Guizhou, and suggest that Guizhou might be a radiation centre of the present form of HTNV.

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2008-08-01
2024-12-04
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References

  1. Arikawa J., Takashima I., Hashimoto N. 1985; Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralizing antibody. Arch Virol 86:303–313 [CrossRef]
    [Google Scholar]
  2. Baek L. J., Kariwa H., Lokugamage K., Yoshimatsu K., Arikawa J., Takashima I., Kang J. I., Moon S. S., Chung S. Y. other authors 2006; Soochong virus: an antigenically and genetically distinct hantavirus isolated from Apodemus peninsulae in Korea. J Med Virol 78:290–297 [CrossRef]
    [Google Scholar]
  3. Blakqori G., Kochs G., Haller O., Weber F. 2003; Functional L polymerase of La Crosse virus allows in vivo reconstitution of recombinant nucleocapsids. J Gen Virol 84:1207–1214 [CrossRef]
    [Google Scholar]
  4. Chen H. X., Luo C. H., Chen F., Wang X. H., Yang J. H., Ma L. J., Hu J. Y., Sun H. Y., Yao Z. H., Qiu J. C. 1999; Surveillance on the hemorrhagic fever with renal syndrome in China. Chin J Public Health 15:616–623 (in Chinese).
    [Google Scholar]
  5. Childs J. E., Glass G. E., Korch G. W., LeDuc J. W. 1989; Effects of hantaviral infection on survival, growth and fertility in wild rat ( Rattus norvegicus ) populations of Baltimore. Maryland. J Wildl Dis 25:469–476 [CrossRef]
    [Google Scholar]
  6. Flick R., Pettersson R. F. 2001; Reverse genetics system for Uukuniemi virus ( Bunyaviridae ): RNA polymerase I-catalyzed expression of chimeric viral RNAs. J Virol 75:1643–1655 [CrossRef]
    [Google Scholar]
  7. Flick K., Hooper J. W., Schmaljohn C. S., Pettersson R. F., Feldmann H., Flick R. 2003; Rescue of hantaan virus minigenomes. Virology 306:219–224 [CrossRef]
    [Google Scholar]
  8. Henderson W. W., Monroe M. C., St Jeor S. C., Thayer W. P., Rowe J. E., Peters C. J., Nichol S. T. 1995; Naturally occurring Sin Nombre virus genetic reassortants. Virology 214:602–610 [CrossRef]
    [Google Scholar]
  9. Holmes E. C., Woelk C. H., Kassis R., Bourhy H. 2002; Genetic constraints and the adaptive evolution of rabies virus in nature. Virology 292:247–257 [CrossRef]
    [Google Scholar]
  10. Hughes A. L., Friedman R. 2000; Evolutionary diversification of protein-coding genes of hantaviruses. Mol Biol Evol 17:1558–1568 [CrossRef]
    [Google Scholar]
  11. Hutchinson K. L., Rollin P. E., Shieh W. J., Zaki S., Greer P. W., Peters C. J. 2000; Transmission of Black Creek Canal virus between cotton rats. J Med Virol 60:70–76 [CrossRef]
    [Google Scholar]
  12. Johnson K. M. 1999; Introduction. In Manual of Hemorrhagic Fever with Renal Syndrome and Hantavirus Pulmonary Syndrome. WHO Collaborating Center for Virus Reference and Research (Hantaviruses) pp 1–6Edited by Lee H. W., Calisher C., Schmaljohn C. Seoul: Seoul Asan Institute for Life Sciences;
    [Google Scholar]
  13. Kaukinen P., Vaheri A., Plyusnin A. 2005; Hantavirus nucleocapsid protein: a multifunctional molecule with both housekeeping and ambassadorial duties. Arch Virol 150:1693–1713 [CrossRef]
    [Google Scholar]
  14. Khaiboullina S. F., Morzunov S. P., St Jeor S. C. 2005; Hantaviruses: molecular biology, evolution and pathogenesis. Curr Mol Med 5:773–790 [CrossRef]
    [Google Scholar]
  15. Klempa B., Fichet-Calvet E., Lecompte E., Auste B., Aniskin V., Meisel H., Denys C., Koivogui L., ter Meulen J., Kruger D. H. 2006; Hantavirus in African wood mouse, Guinea. Emerg Infect Dis 12:838–840 [CrossRef]
    [Google Scholar]
  16. Kurata T., Tsai T. F., Bauer S. P., McCormick J. B. 1983; Immunofluorescence studies of disseminated hantaan virus infection of suckling mice. Infect Immun 41:391–398
    [Google Scholar]
  17. Lee H. W. 1999; Virus isolation. In Manual of Hemorrhagic Fever with Renal Syndrome and Hantavirus Pulmonary Syndrome. WHO Collaborating Center for Virus Reference and Research (Hantaviruses) pp 74–79Edited by Lee H. W., Calisher C., Schmaljohn C. Seoul: Seoul Asan Institute for Life Sciences;
    [Google Scholar]
  18. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. J Infect Dis 137:298–308 [CrossRef]
    [Google Scholar]
  19. Li D., Schmaljohn A. L., Anderson K., Schmaljohn C. S. 1995; Entire nucleotide sequences of the M and S segments of two hantavirus isolates from California: evidence for reassortment in nature among viruses related to hantavirus pulmonary syndrome. Virology 206:973–983 [CrossRef]
    [Google Scholar]
  20. Li D. H., Luo R., Song X. Z. 1999; Zoogeographical divisions of rodents in Guizhou province. Dong Wu Xue Bao 45:268–278
    [Google Scholar]
  21. Li L., Yang D. J., Chen J. Y., Ding J. Q., Su X., Tian Y. Q., Wei J. F. 2005; Cloning and sequencing of S segment of hantavirus strain TJJ16 isolated in Tianjin. Chin J Microbiol Immunol 25:652–655
    [Google Scholar]
  22. Liang M., Li D., Xiao S. Y., Hang C., Rossi C. A., Schmaljohn C. S. 1994; Antigenic and molecular characterization of hantavirus isolates from China. Virus Res 31:219–233 [CrossRef]
    [Google Scholar]
  23. Liu X., Wei F., Li M., Jiang X., Feng Z., Hu J. 2004; Molecular phylogeny and taxonomy of wood mice (genus Apodemus Kaup, 1829) based on entire mtDNA cytochrome b sequences, with emphasis on Chinese species. Mol Phylogenet Evol 33:1–15 [CrossRef]
    [Google Scholar]
  24. Lokugamage K., Kariwa H., Lokugamage N., Miyamoto H., Iwasa M., Hagiya T., Araki K., Tachi A., Mizutani T. other authors 2004; Genetic and antigenic characterization of the Amur virus associated with hemorrhagic fever with renal syndrome. Virus Res 101:127–134 [CrossRef]
    [Google Scholar]
  25. Morzunov S. P., Rowe J. E., Ksiazek T. G., Peters C. J., St Jeor S. C., Nichol S. T. 1998; Genetic analysis of the diversity and origin of hantaviruses in Peromyscus leucopus mice in North America. J Virol 72:57–64
    [Google Scholar]
  26. Musser G. G., Brothers E. M., Carleton M. D., Hutterer R. 1996; Taxonomy and distributional records of Oriental and European Apodemus , with a review of the Apodemus / Sylvaemu s problem. Bonn Zool Beitr 46:143–190
    [Google Scholar]
  27. Netski D., Thran B. H., St Jeor S. C. 1999; Sin Nombre virus pathogenesis in Peromyscus maniculatus . J Virol 73:585–591
    [Google Scholar]
  28. Nichol S. T., Beaty B. J., Elliott R. M., Goldbach R., Plyusnin A., Schmaljohn C. S., Tesh R. B. 2005; Bunyaviridae. In Virus Taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses pp 695–716Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball. Amsterdam: Elsevier Academic Press;
    [Google Scholar]
  29. Ogino M., Yoshimatsu K., Ebihara H., Araki K., Lee B. H., Okumura M., Arikawa J. 2004; Cell fusion activities of hantaan virus envelope glycoproteins. J Virol 78:10776–10782 [CrossRef]
    [Google Scholar]
  30. Okuno Y., Yamanishi K., Takahashi Y., Tanishita O., Nagai T., Dantas J. R. Jr, Okamoto Y., Tadano M., Takahashi M. 1986; Haemagglutination-inhibition test for haemorrhagic fever with renal syndrome using virus antigen prepared from infected tissue culture fluid. J Gen Virol 67:149–156 [CrossRef]
    [Google Scholar]
  31. Plyusnin A., Morzunov S. P. 2001; Virus evolution and genetic diversity of hantaviruses and their rodent hosts. Curr Top Microbiol Immunol 256:47–75
    [Google Scholar]
  32. Plyusnin A., Vapalahti O., Vaheri A. 1996; Hantaviruses: genome structure, expression and evolution. J Gen Virol 77:2677–2687 [CrossRef]
    [Google Scholar]
  33. Puthavathana P., Lee H. W., Kang C. Y. 1992; Typing of hantaviruses from five continents by polymerase chain reaction. Virus Res 26:1–14 [CrossRef]
    [Google Scholar]
  34. Razzauti M., Plyusnina A., Henttonen H., Plyusnin A. 2008; Accumulation of point mutations and reassortment of genomic RNA segments are involved in the microevolution of Puumala hantavirus in a bank vole ( Myodes glareolus ) population. J Gen Virol 89:1649–1660 [CrossRef]
    [Google Scholar]
  35. Rizvanov A. A., Khaiboullina S. F., St Jeor S. 2004; Development of reassortant viruses between pathogenic hantavirus strains. Virology 327:225–232 [CrossRef]
    [Google Scholar]
  36. Rodriguez L. L., Owens J. H., Peters C. J., Nichol S. T. 1998; Genetic reassortment among viruses causing hantavirus pulmonary syndrome. Virology 242:99–106 [CrossRef]
    [Google Scholar]
  37. Schmaljohn C., Hjelle B. 1997; Hantaviruses: a global disease problem. Emerg Infect Dis 3:95–104 [CrossRef]
    [Google Scholar]
  38. Schmaljohn C. S., Jennings G. B., Hay J., Dalrymple J. M. 1986; Coding strategy of the S genome segment of hantaan virus. Virology 155:633–643 [CrossRef]
    [Google Scholar]
  39. Serizawa K., Suzuki H., Tsuchiya K. 2000; A phylogenetic view on species radiation in Apodemus inferred from variation of nuclear and mitochondrial genes. Biochem Genet 38:27–40 [CrossRef]
    [Google Scholar]
  40. Shi X., Liang M., Hang C., Song G., McCaughey C., Elliott R. M. 1998; Nucleotide sequence and phylogenetic analysis of the medium (M) genomic RNA segments of three hantaviruses isolated in China. Virus Res 56:69–76 [CrossRef]
    [Google Scholar]
  41. Sironen T., Vaheri A., Plyusnin A. 2001; Molecular evolution of Puumala hantavirus. J Virol 75:11803–11810 [CrossRef]
    [Google Scholar]
  42. Sun C. Q., Chen L. F., Zhang B. S., Liu Y. C., Cui Y., Wu Y. H., Xu J., Li J. H., Liu Z. W. other authors 2001; Complete nucleotide sequence of the S genome segment of hantavirus HTN261 strain isolated in the northeast China. Virol Sin 16:140–145 (in Chinese
    [Google Scholar]
  43. Sun L., Zhang Y. Z., Li L. H., Zhang Y. P., Zhang A. M., Hao Z. Y., Sun J. W., Chen H. X. 2005; Genetic subtypes and distribution of Seoul virus in Henan. Zhonghua Liu Xing Bing Xue Za Zhi 26:578–582 (in Chinese
    [Google Scholar]
  44. Suzuki H., Sato J. J., Tsuchiya K., Luo J., Zhang Y. P., Wang Y. X., Jiang X. L. 2003; Molecular phylogeny of wood mice ( Apodemus , Muridae) in East Asia. Biol J Linn Soc 80:469–481 [CrossRef]
    [Google Scholar]
  45. Tischler N. D., Gonzalez A., Perez-Acle T., Rosemblatt M., Valenzuela P. D. 2005; Hantavirus Gc glycoprotein: evidence for a class II fusion protein. J Gen Virol 86:2937–2947 [CrossRef]
    [Google Scholar]
  46. Tsai T. F., Tang Y. W., Hu S. L., Ye K. L., Chen G. L., Xu Z. Y. 1984; Hemagglutination-inhibiting antibody in hemorrhagic fever with renal syndrome. J Infect Dis 150:895–898 [CrossRef]
    [Google Scholar]
  47. Valarcher J. F., Schelcher F., Bourhy H. 2000; Evolution of bovine respiratory syncytial virus. J Virol 74:10714–10728 [CrossRef]
    [Google Scholar]
  48. Wang Z. X., Lu D. Q., Lu D. F., Yan F. Z., Liao Z. S., Li C. J., Fu D. Q., Wang Q. C., Wang Q. H., Hu Y. 1989; Primary study on epidemic hemorrhagic fever (EHF) in Guizhou. Zhonghua Liu Xing Bing Xue Za Zhi 10:101–105 (in Chinese
    [Google Scholar]
  49. Wang H., Yoshimatsu K., Ebihara H., Ogino M., Araki K., Kariwa H., Wang Z., Luo Z., Li D. other authors 2000; Genetic diversity of hantaviruses isolated in China and characterization of novel hantaviruses isolated from Niviventer confucianus and Rattus rattus . Virology 278:332–345 [CrossRef]
    [Google Scholar]
  50. Wang D. M., Wang Z. X., Tong Y. B., Liu M., Cai X. H., Hu L. J., Huang Y. P. 2003; Surveillance on hemorrhagic fever with renal syndrome in Guizhou during 1984–2000. Zhonghua Liu Xing Bing Xue Za Zhi 24:694–696 (in Chinese
    [Google Scholar]
  51. Xia W. P. 1984; A study on Chinese Apodemus with a discussion of its relations to Japanese species. Acta Theriol Sin 4:93–98 (in Chinese
    [Google Scholar]
  52. Yang Z., Bielawski J. P. 2000; Statistical methods for detecting molecular adaptation. Trends Ecol Evol 15:496–503 [CrossRef]
    [Google Scholar]
  53. Yao Z. H., Dong G. M., Yu Y. X., Jia K. L., Yan Y. C. 2001a; Molecular characteristics of the seed virus of HFRS candidate vaccine A16 strain. Virol Sin 16:315–320 (in Chinese
    [Google Scholar]
  54. Yao Z. H., Yu Y. X., Dong G. M., Liu W. X., Yang H. J., Ding X. H. 2001b; Complete genome sequence analysis of the hantavirus Z10 strain. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 15:112–115 (in Chinese
    [Google Scholar]
  55. Yao Z. H., Dong G. M., Yu Y. X., Zhang J. K., Yan D. Y., Liu X. C., Zhang L. L. 2002; A new subtype of hantavirus SN7 isolated from Niviventer confucianus in Sichuan province, China. Chin J Infect Dis 20:79–82 (in Chinese
    [Google Scholar]
  56. Zhang Y. Z., Xiao D. L., Wang Y., Wang H. X., Sun L., Tao X. X., Qu Y. G. 2004; The epidemic characteristics and preventive measures of hemorrhagic fever with syndromes in China. Zhonghua Liu Xing Bing Xue Za Zhi 25:466–469 (in Chinese
    [Google Scholar]
  57. Zhang Y. Z., Zou Y., Yao L. S., Hu G. W., Du Z. S., Jin L. Z., Liu Y. Y., Wang H. X., Chen X. other authors 2007; Isolation and characterization of hantavirus carried by Apodemus peninsulae in Jilin, China. J Gen Virol 88:1295–1331 [CrossRef]
    [Google Scholar]
  58. Zou Y., Hu J., Wang Z. X., Wang D. M., Yu C., Zhou J. Z., Li M. H., Fu Z. F., Zhang Y. Z. 2008; Genetic characterization of hantaviruses isolated from Guizhou, China: evidence for spillover and reassortment in nature. J Med Virol 80:1033–1041 [CrossRef]
    [Google Scholar]
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