1887

Abstract

The UL15, UL28 and UL33 proteins of herpes simplex virus type 1 (HSV-1) are thought to comprise a terminase complex responsible for cleavage and packaging of the viral genome into pre-assembled capsids. Immunofluorescence studies confirmed that shortly after infection with wild-type HSV-1 these three proteins localize to viral DNA replication compartments within the nucleus, identified by the presence of the single-stranded DNA-binding protein, ICP8. In cells infected with either UL28- or UL33-null mutants, the other two terminase proteins also co-localized with ICP8. In contrast, neither UL28 nor UL33 was detectable in replication compartments following infection with a UL15-null mutant, although Western blot analysis showed they were present in normal amounts in the infected cells. Provision of UL15 in a complementing cell line restored the ability of all three proteins to localize to replication compartments. These data indicate that UL15 plays a key role in localizing the terminase complex to DNA replication compartments, and that it can interact independently with UL28 and UL33.

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2008-07-01
2024-04-20
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References

  1. Abbotts A. P., Preston V. G., Hughes M., Patel A. H., Stow N. D. 2000; Interaction of the herpes simplex virus type 1 packaging protein UL15 with full length and truncated forms of UL28. J Gen Virol 81:2999–3009
    [Google Scholar]
  2. Adelman K., Salmon B., Baines J. 2001; Herpes simplex virus packaging sequences adopt novel structures that are specifically recognised by a component of the cleavage and packaging machinery. Proc Natl Acad Sci U S A 98:3086–3091 [CrossRef]
    [Google Scholar]
  3. Baines J. D., Weller S. K. 2005; Cleavage and packaging of herpes simplex virus 1 DNA. In Viral Genome Packaging Machines: Genetics, Structure, and Mechanism pp 135–150Edited by Catalano C. E. New York: Kluwer Academic/Plenum Publishers;
    [Google Scholar]
  4. Baines J. D., Cunningham C., Nalwanga D., Davison A. J. 1997; The UL15 gene of herpes simplex virus type 1 contains within its second exon a novel open reading frame that is translated in frame with the UL15 gene product. J Virol 71:2666–2673
    [Google Scholar]
  5. Beard P. M., Taus N. S., Baines J. D. 2002; DNA cleavage and packaging proteins encoded by the genes UL15, UL28 and UL33 of herpes simplex virus type 1 form a complex in infected cells. J Virol 76:4785–4791 [CrossRef]
    [Google Scholar]
  6. Brown J. C., McVoy M. A., Homa F. L. 2002; Packaging DNA into herpesvirus capsids. In Structure–Function Relationships of Human Pathogenic Viruses pp 111–153Edited by Holzenburg A., Bogner E. New York: Kluwer Academic/Plenum Publishers;
    [Google Scholar]
  7. Cunningham C., Davison A. J. 1993; A cosmid-based system for constructing mutants of herpes simplex virus type 1. Virology 197:116–124 [CrossRef]
    [Google Scholar]
  8. Davison A. J. 1992; Channel catfish virus: a new type of herpesvirus. Virology 186:9–14 [CrossRef]
    [Google Scholar]
  9. de Bruyn Kops A., Uprichard S. L., Chen M., Knipe D. M. 1998; Comparison of the intranuclear distributions of herpes simplex virus proteins involved in various viral functions. Virology 252:162–178 [CrossRef]
    [Google Scholar]
  10. Everett R. D., Sourvinos G., Leiper C., Clements J. B., Orr A. 2004; Formation of nuclear foci of the herpes simplex virus type 1 regulatory protein ICP4 at early times of infection: localization, dynamics, recruitment of ICP27, and evidence for the de novo induction of ND10-like complexes. J Virol 78:1903–1917 [CrossRef]
    [Google Scholar]
  11. Jacobson J. G., Yang K., Baines J., Homa F. L. 2006; Linker insertion mutations in the herpes simplex virus type 1 UL28 gene: effects on UL28 interaction with UL15 and UL33 and identification of a second-site mutation in the UL15 gene that suppresses a lethal UL28 mutation. J Virol 80:12312–12323 [CrossRef]
    [Google Scholar]
  12. Koslowski K. M., Shaver P. R., Wang X.-Y., Tenney D. J., Pedersen N. E. 1997; The pseudorabies virus protein UL28 enters the nucleus after co-expression with the herpes simplex virus UL15 protein. J Virol 71:9118–9123
    [Google Scholar]
  13. Koslowski K. M., Shaver P. R., Casey J. T. I., Wilson T., Yamanaka G., Sheaffer A. K., Tenney D. J., Pedersen N. E. 1999; Physical and functional interactions between the herpes simplex virus UL15 and UL28 DNA cleavage and packaging proteins. J Virol 73:1704–1707
    [Google Scholar]
  14. Lamberti C., Weller S. K. 1998; The herpes simplex virus type 1 cleavage/packaging protein, UL32, is involved in efficient localisation of capsids to replication compartments. J Virol 72:2463–2473
    [Google Scholar]
  15. Porter I. M., Stow N. D. 2004; Replication, recombination and packaging of amplicon DNA in cells infected with the herpes simplex virus type 1 alkaline nuclease mutant amb UL12. J Gen Virol 85:3501–3510 [CrossRef]
    [Google Scholar]
  16. Przech A. J., Yu D., Weller S. K. 2003; Point mutations in exon 1 of the herpes simplex virus putative terminase subunit, UL15, indicate that most conserved residues are essential for cleavage and packaging. J Virol 77:9613–9621 [CrossRef]
    [Google Scholar]
  17. Quinlan M. P., Chen L. B., Knipe D. M. 1984; The intranuclear location of a herpes simplex virus DNA-binding protein is determined by the status of viral DNA replication. Cell 36:857–868 [CrossRef]
    [Google Scholar]
  18. Reynolds A. E., Fan Y., Baines J. D. 2000; Characterisation of the UL33 gene product of herpes simplex virus 1. Virology 266:310–318 [CrossRef]
    [Google Scholar]
  19. Strang B. L., Stow N. D. 2005; Circularization of the herpes simplex virus type 1 genome upon lytic infection. J Virol 79:12487–12494 [CrossRef]
    [Google Scholar]
  20. Taus N. S., Salmon B., Baines J. D. 1998; The herpes simplex virus 1 UL17 gene is required for localisation of capsids and major and minor capsid proteins to intranuclear sites where viral DNA is cleaved and packaged. Virology 252:115–125 [CrossRef]
    [Google Scholar]
  21. Tengelsen L. A., Pederson N. E., Shaver P. R., Wathen M. W., Homa F. L. 1993; Herpes simplex virus type 1 DNA cleavage and encapsidation require the product of the UL28 gene: isolation and characterization of two UL28 deletion mutants. J Virol 67:3470–3480
    [Google Scholar]
  22. White C. A., Stow N. D., Patel A. H., Hughes M., Preston V. G. 2003; Herpes simplex virus type 1 portal protein UL6 interacts with the putative terminase subunits UL15 and UL28. J Virol 77:6351–6358 [CrossRef]
    [Google Scholar]
  23. Yang K., Baines J. D. 2006; The putative terminase subunit of herpes simplex virus type 1 encoded by UL28 is necessary and sufficient to mediate interaction between pUL15 and pUL33. J Virol 80:5733–5739 [CrossRef]
    [Google Scholar]
  24. Yang K., Homa F., Baines J. D. 2007; Putative terminase subunits of herpes simplex virus 1 form a complex in the cytoplasm and interact with portal protein in the nucleus. J Virol 81:6419–6433 [CrossRef]
    [Google Scholar]
  25. Yu D., Weller S. K. 1998a; Genetic analysis of the UL15 locus for the putative terminase of herpes simplex virus type 1. Virology 243:32–44 [CrossRef]
    [Google Scholar]
  26. Yu D., Weller S. K. 1998b; Herpes simplex virus type 1 cleavage and packaging proteins UL15 and UL28 are associated with B but not C capsids during packaging. J Virol 72:7428–7439
    [Google Scholar]
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