1887

Abstract

The role of the UL49 gene product, VP22, of bovine herpesvirus type 1 (BoHV-1) in virus replication was characterized with respect to a putative functional interaction of VP22 with the viral glycoprotein E (gE) during BoHV-1 cell-to-cell spread. Deletion of the open reading frames of UL49 and/or gE from an infectious BoHV-1 bacterial artificial chromosome clone did not severely impair the production of viral progeny in single-step growth experiments. However, plaque sizes induced by a VP22-negative BoHV-1 were reduced by 52 %, whilst for the gE/VP22-negative double-deletion mutant a reduction of 83 % could be observed in comparison with parental and revertant viruses, which was consistent with a marked reduction in multi-step growth experiments at early time points. These results suggest that gE and VP22 are important for BoHV-1 cell-to-cell spread, and that both are likely to act independently of each other in a critical pathway for virus cell-to-cell spread.

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2008-09-01
2024-04-24
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References

  1. Arvin A. M. 1996; Varicella-zoster virus. Clin Microbiol Rev 9:361–381
    [Google Scholar]
  2. Chi J. H., Harley C. A., Mukhopadhyay A., Wilson D. W. 2005; The cytoplasmic tail of herpes simplex virus envelope glycoprotein D binds to the tegument protein VP22 and to capsids. J Gen Virol 86:253–261 [CrossRef]
    [Google Scholar]
  3. Corey L., Spear P. G. 1986; Infections with herpes simplex viruses (1). N Engl J Med 314:686–691 [CrossRef]
    [Google Scholar]
  4. del Rio T., Werner H. C., Enquist L. W. 2002; The pseudorabies virus VP22 homologue (UL49) is dispensable for virus growth in vitro and has no effect on virulence and neuronal spread in rodents. J Virol 76:774–782 [CrossRef]
    [Google Scholar]
  5. Dietz P., Klupp B. G., Fuchs W., Köllner B., Weiland E., Mettenleiter T. C. 2000; Pseudorabies virus glycoprotein K requires the UL20 gene product for processing. J Virol 74:5083–5090 [CrossRef]
    [Google Scholar]
  6. Dorange F., Tischer B. K., Vautherot J. F., Osterrieder N. 2002; Characterization of Marek's disease virus serotype 1 (MDV-1) deletion mutants that lack UL46 to UL49 genes: MDV-1 UL49, encoding VP22, is indispensable for virus growth. J Virol 76:1959–1970 [CrossRef]
    [Google Scholar]
  7. Duffy C., Lavail J. H., Tauscher A. N., Wills E. G., Blaho J. A., Baines J. D. 2006; Characterization of a UL49-null mutant: VP22 of herpes simplex virus type 1 facilitates viral spread in cultured cells and the mouse cornea. J Virol 80:8664–8675 [CrossRef]
    [Google Scholar]
  8. Elliott G., Hafezi W., Whiteley A., Bernard E. 2005; Deletion of the herpes simplex virus VP22-encoding gene (UL49) alters the expression, localization, and virion incorporation of ICP0. J Virol 79:9735–9745 [CrossRef]
    [Google Scholar]
  9. Engelhardt T., Keil G. M. 1996; Identification and characterization of the bovine herpesvirus 5 US4 gene and gene products. Virology 225:126–135 [CrossRef]
    [Google Scholar]
  10. Farnsworth A., Wisner T. W., Johnson D. C. 2007; Cytoplasmic residues of herpes simplex virus glycoprotein gE required for secondary envelopment and binding of tegument proteins VP22 and UL11 to gE and gD. J Virol 81:319–331 [CrossRef]
    [Google Scholar]
  11. Fauquet C. M., Majo M. A., Maniloff J., Desselberger U., Ball L. 2005 Virus Taxonomy: Classification and Nomenclature of Viruses. Eighth Report of the International Committee on the Taxonomy of Viruses London: Elsevier Academic Press;
    [Google Scholar]
  12. Field H. J., Hill T. J. 1975; The pathogenesis of pseudorabies in mice: virus replication at the inoculation site and axonal uptake. J Gen Virol 26:145–148 [CrossRef]
    [Google Scholar]
  13. Fuchs W., Klupp B. G., Granzow H., Hengartner C., Brack A., Mundt A., Enquist L. W., Mettenleiter T. C. 2002; Physical interaction between envelope glycoproteins E and M of pseudorabies virus and the major tegument protein UL49. J Virol 76:8208–8217 [CrossRef]
    [Google Scholar]
  14. Gibbs E. P. J., Rweyemamu M. M. 1977; Bovine herpesviruses. Part 1. Vet Bull 47:317–343
    [Google Scholar]
  15. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K. O., Kaplan A. S. 1984; Characterization of the envelope proteins of pseudorabies virus. J Virol 52:583–590
    [Google Scholar]
  16. Harms J. S., Ren X., Oliveira S. C., Splitter G. A. 2000; Distinctions between bovine herpesvirus 1 and herpes simplex virus type 1 VP22 tegument protein subcellular associations. J Virol 74:3301–3312 [CrossRef]
    [Google Scholar]
  17. Highlander S. L., Sutherland S. L., Gage P. J., Johnson D. C., Levine M., Glorioso J. C. 1987; Neutralizing monoclonal antibodies specific for herpes simplex virus glycoprotein D inhibit virus penetration. J Virol 61:3356–3364
    [Google Scholar]
  18. Johnson D. C., Huber M. T. 2002; Directed egress of animal viruses promotes cell-to-cell spread. J Virol 76:1–8 [CrossRef]
    [Google Scholar]
  19. Liang X., Chow B., Li Y., Raggo C., Yoo D., Attah-Poku S., Babiuk L. A. 1995; Characterization of bovine herpesvirus 1 UL49 homolog gene and product: bovine herpesvirus 1 UL49 homolog is dispensable for virus growth. J Virol 69:3863–3867
    [Google Scholar]
  20. Liang X., Chow B., Babiuk L. A. 1997; Study of immunogenicity and virulence of bovine herpesvirus 1 mutants deficient in the UL49 homolog, UL49.5 homolog and dUTPase genes in cattle. Vaccine 15:1057–1064 [CrossRef]
    [Google Scholar]
  21. Mallory S., Sommer M., Arvin A. M. 1997; Mutational analysis of the role of glycoprotein I in varicella-zoster virus replication and its effects on glycoprotein E conformation and trafficking. J Virol 71:8279–8288
    [Google Scholar]
  22. Matheka H. D., Straub O. C. 1972; Further investigations to differentiate IBR-virus from IPV-virus by the carrier-free zone electrophoresis in a glucose density gradient. Arch Gesamte Virusforsch 39:265–273 [CrossRef]
    [Google Scholar]
  23. Michael K., Klupp B. G., Mettenleiter T. C., Karger A. 2006; Composition of pseudorabies virus particles lacking tegument protein US3, UL47, or UL49 or envelope glycoprotein E. J Virol 80:1332–1339 [CrossRef]
    [Google Scholar]
  24. Neubauer A., Braun B., Brandmüller C., Kaaden O.-R., Osterrieder N. 1997; Analysis of the contributions of the equine herpesvirus 1 glycoprotein gB homolog to virus entry and direct cell-to-cell spread. Virology 227:281–294 [CrossRef]
    [Google Scholar]
  25. Polcicova K., Goldsmith K., Rainish B. L., Wisner T. W., Johnson D. C. 2005; The extracellular domain of herpes simplex virus gE is indispensable for efficient cell-to-cell spread: evidence for gE/gI receptors. J Virol 79:11990–12001 [CrossRef]
    [Google Scholar]
  26. Rebordosa X., Pinol J., Perez-Pons J. A., Lloberas J., Naval J., Serra-Hartmann X., Espuna E., Querol E. 1996; Glycoprotein E of bovine herpesvirus type 1 is involved in virus transmission by direct cell-to-cell spread. Virus Res 45:59–68 [CrossRef]
    [Google Scholar]
  27. Rijsewijk F. A., Verschuren S. B., Madić J., Ruuls R. C., Renaud P., van Oirschot J. T. 1999; Spontaneous BHV1 recombinants in which the gI/gE/US9 region is replaced by a duplication/inversion of the US1.5/US2 region. Arch Virol 144:1527–1537 [CrossRef]
    [Google Scholar]
  28. Schumacher D., Tischer B. K., Reddy S. M., Osterrieder N. 2001; Glycoproteins E and I of Marek's disease virus serotype 1 are essential for virus growth in cultured cells. J Virol 75:11307–11318 [CrossRef]
    [Google Scholar]
  29. Tikoo S. K., Campos M., Babiuk L. A. 1995; Bovine herpesvirus 1 (BHV-1): biology, pathogenesis, and control. Adv Virus Res 45:191–223
    [Google Scholar]
  30. Trapp S., Osterrieder N., Keil G. M., Beer M. 2003; Mutagenesis of a bovine herpesvirus type 1 genome cloned as an infectious bacterial artificial chromosome: analysis of glycoprotein E and G double deletion mutants. J Gen Virol 84:301–306 [CrossRef]
    [Google Scholar]
  31. van Engelenburg F. A., Kaashoek M. J., Rijsewijk F. A., van den Burg L., Moerman A., Gielkens A. L., van Oirschot J. T. 1994; A glycoprotein E deletion mutant of bovine herpesvirus 1 is avirulent in calves. J Gen Virol 75:2311–2318 [CrossRef]
    [Google Scholar]
  32. Yoshitake N., Xuan X., Otsuka H. 1997; Identification and characterization of bovine herpesvirus-1 glycoproteins E and I. J Gen Virol 78:1399–1403
    [Google Scholar]
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