1887

Abstract

Poliovirus (PV) infection starts with binding to its receptor (PVR), followed by a receptor-aided, temperature-sensitive conformational change of the infectious particle (sedimenting at 160S) to a particle which sediments at 135S. Reported in this communication is the successful incorporation into lipid bilayers of two forms of the receptor: the full-length human receptor and a modified clone in which the extracellular domains of the receptor were fused to a glycosylphosphatidylinositol tail. Addition of virus (160S) to receptor-containing bilayers leads to channel formation, whereas no channels were observed when the receptor-modified viral particle (135S) was added. Increasing the temperature from 21 to 31 °C led to a 10-fold increase in the magnitude of the single channel conductance, which can be interpreted as a conformational change in the channel structure. A mutant PV with an amino acid change in VP4 (one of the coat proteins) which is defective in genome uncoating failed to produce channels, suggesting that VP4 might be involved in the channel architecture. These studies provide the first electrophysiological characterization of the interactions between poliovirus and its receptor incorporated into a lipid bilayer membrane. Furthermore, they form the foundation for future studies aiming at defining the molecular architecture of the virus–receptor complex.

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2004-06-01
2019-10-15
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References

  1. Acosta, J., Hettinga, J., Fluckiger, R., Krumrei, N., Goldfine, A., Angarita, L. & Halperin, J. A. ( 2000; ). Molecular basis for a link between complement and the vascular complications of diabetes. Proc Natl Acad Sci U S A 97, 5450–5455.[CrossRef]
    [Google Scholar]
  2. Andino, R., Silvera, D., Suggett, S. D., Achacoso, P. L., Miller, C. J., Baltimore, D. & Feinberg, M. B. ( 1994; ). Engineering poliovirus as a vaccine vector for the expression of diverse antigens. Science 265, 1448–1451.[CrossRef]
    [Google Scholar]
  3. Arita, M., Koike, S., Aoki, J., Horie, H. & Nomoto, A. ( 1998; ). Interaction of poliovirus with its purified receptor and conformational alteration in the virion. J Virol 72, 3578–3586.
    [Google Scholar]
  4. Brown, D. A. & Rose, J. K. ( 1992; ). Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68, 533–544.[CrossRef]
    [Google Scholar]
  5. Carrasco, L. ( 1995; ). Modification of membrane permeability induced by animal viruses. Adv Virus Res 45, 61–112.
    [Google Scholar]
  6. Coronado, R., Morrissette, J., Sukhareva, M. & Vaughan, D. M. ( 1994; ). Structure and function of ryanodine receptors. Am J Physiol 266, C1485–1504.
    [Google Scholar]
  7. Danthi, P., Tosteson, M. T., Li, Q.-H. & Chow, M. ( 2003; ). Genome delivery and ion channel properties are altered in VP4 mutants of poliovirus. J Virol 77, 5266–5274.[CrossRef]
    [Google Scholar]
  8. de Sena, J. & Mandel, B. ( 1977; ). Studies on the in vitro uncoating of poliovirus. II. Characteristics of the membrane-modified particle. Virology 78, 554–566.[CrossRef]
    [Google Scholar]
  9. Fenwick, M. L. & Cooper, P. D. ( 1962; ). Early interactions between poliovirus and ERK cells: some observations on the nature of the rejected particles. Virology 18, 212–223.[CrossRef]
    [Google Scholar]
  10. Freistadt, M. S., Kaplan, G. & Racaniello, V. R. ( 1990; ). Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues. Mol Cell Biol 10, 5700–5706.
    [Google Scholar]
  11. Gomez Yafal, A., Kaplan, G., Racaniello, V. R. & Hogle, J. M. ( 1993; ). Characterization of poliovirus conformational alteration mediated by soluble cell receptors. Virology 197, 501–505.[CrossRef]
    [Google Scholar]
  12. Hille, B. ( 2001; ). Ionic Channels of Excitable Membranes, 3rd edn. Sunderland, MA: Sinauer Associates.
  13. Joklik, W. K. & Darnell, J. E. Jr ( 1961; ). The adsorption and early fate of purified poliovirus in HeLa cells. Virology 13, 439–447.[CrossRef]
    [Google Scholar]
  14. Kaplan, G., Freistadt, M. S. & Racaniello, V. R. ( 1990; ). Neutralization of poliovirus by cell receptors expressed in insect cells. J Virol 64, 4697–4702.
    [Google Scholar]
  15. Koike, S., Ise, I. & Nomoto, A. ( 1991; ). Functional domains of the poliovirus receptor. Proc Natl Acad Sci U S A 88, 4104–4108.[CrossRef]
    [Google Scholar]
  16. Lonberg-Holm, K. L. B., Gosser, L. B. & Kauer, J. C. ( 1975; ). Early alteration of poliovirus in infected cells and its specific inhibition. J Gen Virol 27, 329–342.[CrossRef]
    [Google Scholar]
  17. Mendelsohn, C. L., Wimmer, E. & Racaniello, V. R. ( 1989; ). Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56, 855–865.[CrossRef]
    [Google Scholar]
  18. Mikoshiba, K., Furuichi, T., Miyawaki, A. & 7 other authors ( 1992; ). The inositol 1,4,5-triphosphate receptor. Ciba Found Symp 164, 17–29.
    [Google Scholar]
  19. Montal, M. & Mueller, P. ( 1972; ). Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci U S A 69, 3561–3566.[CrossRef]
    [Google Scholar]
  20. Moscufo, N. & Chow, M. ( 1992; ). Myristate-protein interactions in poliovirus: interactions of VP4 threonine 28 contribute to the structural conformation of assembly intermediates and the stability of assembled virions. J Virol 66, 6849–6857.
    [Google Scholar]
  21. Moscufo, N., Yafal, A. G., Rogove, A., Hogle, J. M. & Chow, M. ( 1993; ). A mutation in VP4 defines a new step in the late stages of cell entry by poliovirus. J Virol 67, 5075–5078.
    [Google Scholar]
  22. Mueller, S. & Wimmer, E. ( 1998; ). Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames. J Virol 72, 20–31.
    [Google Scholar]
  23. Nelson, N., Anholt, R., Lindstrom, J. & Montal, M. ( 1980; ). Reconstitution of purified acetylcholine receptors with functional ion channels in planar lipid bilayers. Proc Natl Acad Sci U S A 77, 3057–3061.[CrossRef]
    [Google Scholar]
  24. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. ( 1992; ). Baculovirus Expression Vectors: a Laboratory Manual. New York: Freeman and Co.
  25. Rueckert, R. ( 1990; ). Picornaviridae and their replication. In Virology, 2nd edn, pp. 507–548. Edited by B. N. Fields & D. Knipe. New York: Raven Press.
  26. Simons, J., Rogove, A., Moscufo, N., Reynolds, C. & Chow, M. ( 1993; ). Efficient analysis of nonviable poliovirus capsid mutants. J Virol 67, 1734–1738.
    [Google Scholar]
  27. Tashmukhamedov, B. A., Makhmudova, E. M., Usmanov, P. B. & Kazakov, I. ( 1985; ). Reconstitution in bilayer lipid membranes of the crab Potamon transcaspicum spider venom sensitive glutamate receptors. Gen Physiol Biophys 4, 625–630.
    [Google Scholar]
  28. Tosteson, M. T. & Chow, M. ( 1997; ). Characterization of the ion channels formed by poliovirus in planar lipid membranes. J Virol 71, 507–511.
    [Google Scholar]
  29. Tosteson, M. T., Tosi, P. F., Nicolau, C. & Tosteson, D. C. ( 1991; ). CD4+ lipid bilayers. A model for human immunodeficiency virus type 1 coat protein binding. J Biol Chem 266, 11433–11435.
    [Google Scholar]
  30. Tosteson, M. T., Kim, J. B., Goldstein, D. J. & Tosteson, D. C. ( 2001; ). Ion channels formed by transcription factors recognize consensus DNA sequences. Biochim Biophys Acta 1510, 209–218.[CrossRef]
    [Google Scholar]
  31. Tsang, S. K., McDermott, B. M., Racaniello, V. & Hogle, J. M. ( 2001; ). Kinetic analysis of the effect of poliovirus receptor on viral uncoating: the receptor as a catalyst. J Virol 75, 4984–4989.[CrossRef]
    [Google Scholar]
  32. Wei Liu, E., Litwack, D., Stanley, M. J., Langford, J. K., Lander, A. D. & Sanderson, R. D. ( 1998; ). Heparan sulfate proteoglycans as adhesive and anti-invasive molecules. Syndecans and glypican have distinct functions. J Biol Chem 273, 22825–22832.[CrossRef]
    [Google Scholar]
  33. Wengler, G., Koschinski, A., Wengler, G. & Dreyer, F. ( 2003; ). Entry of alphaviruses at the plasma membrane converts the viral surface proteins into an ion-permeable pore that can be detected by electrophysiological analyses of whole-cell membrane currents. J Gen Virol 84, 173–181.[CrossRef]
    [Google Scholar]
  34. Zibert, A., Selinka, H. C., Elroy-Stein, O. & Wimmer, E. ( 1992; ). The soluble form of two N-terminal domains of the poliovirus receptor is sufficient for blocking viral infection. Virus Res 25, 51–61.[CrossRef]
    [Google Scholar]
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