1887

Journal of General Virology header logo

Volume 85, Issue 8

A comparison of the effects of oral inoculation with Rotashield and pentavalent reassortant rotavirus vaccine (WC3-PV) on suckling CB17 mice Free

Abstract

The effects of oral inoculation into infant CB17 mice of two reassortant rotavirus vaccines were compared. The vaccines were Rotashield and WC3-PV, a mixture of five reassortants (G1, G2, G3, G4 and P1; pentavalent reassortant vaccine). Control mice were inoculated with a placebo. At 6 days post-inoculation (p.i.), 8 of 13 (62 %; <0·005) Rotashield-inoculated mice developed hepatitis and/or bile-duct obstruction compared with none of 11 mice given WC3-PV and none of 14 given placebo. In the Rotashield-inoculated mice, only serotype G3 rhesus rotavirus (RRV) was isolated from multiple sites, including intestine, liver, pancreas, spleen, blood and mesenteric lymph nodes. Recovery of RRV from Rotashield-inoculated mice followed a biphasic pattern. The two peaks of RRV recovery appeared to coincide firstly with replication in the intestine during days 1–3 p.i., and secondly with virus infection of the liver from days 10 to 15 p.i. WC3 reassortants of four different serotypes were detected only at day 1 p.i. in the intestine, liver, pancreas and blood cells from three WC3-PV-inoculated mouse pups. However, WC3-PV did not produce any hepatopathology. Rotashield and WC3-PV appeared to exhibit different biological activity in infant CB17 mouse pups.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19665-0
2004-08-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/8/vir852245.html?itemId=/content/journal/jgv/10.1099/vir.0.19665-0&mimeType=html&fmt=ahah

References

  1. American Academy of Pediatrics 1998; Prevention of rotavirus disease: guidelines for use of rotavirus vaccine. Pediatrics 102:1483–1491 [CrossRef]
     [Google Scholar]
  2. Blutt S. E., Kirkwood C. D., Parreno V., Warfield K. L., Ciarlet M., Estes M. K., Bok K., Bishop R. F., Conner M. E. 2003; Rotavirus antigenaemia and viraemia: a common event?. Lancet 362:1445–1449 [CrossRef]
     [Google Scholar]
  3. Centers for Disease Control and Prevention; 1999a; Intussusception among recipients of rotavirus vaccine – United States, 1998–1999. JAMA (J Am Med Assoc) 282:520–521 [CrossRef]
     [Google Scholar]
  4. Centers for Disease Control and Prevention (1999b). Withdrawal of rotavirus vaccine recommendation. MMWR Morb Mortal Wkly Rep 48:1007
     [Google Scholar]
  5. Christy C., Offit P., Clark H. F., Treanor J. 1993; Evaluation of a bovine–human rotavirus reassortant vaccine in infants. J Infect Dis 168:1598–1599 [CrossRef]
     [Google Scholar]
  6. Clark H. F., Offit P. A., Ellis R. W., Krah D., Shaw A. R., Eiden J. J., Pichichero M., Treanor J. J. 1996a; WC3 reassortant vaccines in children. Arch Virol Suppl 12:187–198
     [Google Scholar]
  7. Clark H. F., Offit P. A., Ellis R. W. & 8 other authors (1996b). The development of multivalent bovine rotavirus (strain WC3) reassortant vaccine for infants. J Infect Dis 174:S73–S80 [CrossRef]
     [Google Scholar]
  8. Coulson B. S., Witterick P. D., Tan Y., Hewish M. J., Mountford J. N., Harrison L. C., Moneyman M. C. 2002; Growth of rotaviruses in primary pancreatic cells. J Virol 76:9537–9544 [CrossRef]
     [Google Scholar]
  9. Dharakul T., Riepenhoff-Talty M., Albini B., Ogra P. L. 1988; Distribution of rotavirus antigen in intestinal lymphoid tissues: potential role in the development of the mucosal immune response to rotavirus. Clin Exp Immunol 74:14–19
     [Google Scholar]
  10. Dolan K. T., Twist E. M., Horton-Slight P., Forrer C., Bell L. M. Jr, Plotkin S. A., Clark H. F. 1985; Epidemiology of rotavirus electropherotypes determined by a simplified diagnostic technique with RNA analysis. J Clin Microbiol 21:753–758
     [Google Scholar]
  11. Hoshino Y., Saif L. J., Kang S. Y., Sereno M. M., Chen W. K., Kapikian A. Z. 1995; Identification of group A rotavirus genes associated with virulence of a porcine rotavirus and host range restriction of a human rotavirus in the gnotobiotic piglet model. Virology 209:274–280 [CrossRef]
     [Google Scholar]
  12. Hoshino Y., Wagner M., Yan X., Perez-Schael I., Kapikian A. Z. 2003; Horizontal transmission of rhesus monkey rotavirus-based quadrivalent vaccine during a phase 3 clinical trial in Caracas, Venezuela. J Infect Dis 187:791–800 [CrossRef]
     [Google Scholar]
  13. Kobaysshi M., Thompson J., Tollefson S. J., Reed G. W., Wright P. F. 1994; Tetravalent rhesus rotavirus vaccine in young infants. J Infect Dis 170:1260–1263 [CrossRef]
     [Google Scholar]
  14. Moser C. A., Dolfi D. V., Di Vietro M. L., Heaton P. A., Offit P. A., Clark H. F. 2001; Hypertrophy, hyperplasia, and infectious virus in gut-associated lymphoid tissue of mice after oral inoculation with simian–human or bovine–human reassortant rotaviruses. J Infect Dis 183:1108–1111 [CrossRef]
     [Google Scholar]
  15. Mossel E. C., Ramig R. F. 2002; Rotavirus genome segment 7 (NSP3) is a determination of extraintestinal spread in the neonatal mouse. J Virol 76:6502–6509 [CrossRef]
     [Google Scholar]
  16. Offit P. A., Clark H. F., Stroop W. G., Twist E. M., Plotkin S. A. 1983; The cultivation of human rotavirus, strain ‘Wa’, to high titer in cell culture and characterization of the viral structural polypeptides. J Virol Methods 7:29–40 [CrossRef]
     [Google Scholar]
  17. Petersen C., Bruns E., Kuske M., von Wussow P. 1997; Treatment of extrahepatic biliary atresia with interferon-alpha in a murine infectious model. Pediatr Res 42:623–628 [CrossRef]
     [Google Scholar]
  18. Petersen C., Kuske M., Bruns E., Biermanns D., von Wussow P., Mildenberger H. 1998; Progress in developing animal model for biliary atresia. Eur J Pediatr Surg 8:137–141 [CrossRef]
     [Google Scholar]
  19. Riepenhoff-Talty M., Schaekel K., Clark H. F., Mueller W., Uhnoo I., Rossi T., Fisher J., Ogra P. L. 1993; Group A rotavirus produce extrahepatic biliary obstruction in orally inoculated newborn mice. Pediatr Res 33:394–399
     [Google Scholar]
  20. Uhnoo I., Riepenhoff-Talty M., Dharakul T., Chegas P., Fisher J. E., Greenberg H. B., Ogra P. L. 1990; Extramucosal spread and development of hepatitis in immunodeficient and normal mice infected with rhesus rotavirus. J Virol 64:361–368
     [Google Scholar]
  21. Ward R. L., Dinsmore A. M., Goldberg G., Sander D. S., Rappaport R. S., Zito E. T. 1998; Shedding of rotavirus after administration of the tetravalent rhesus rotavirus vaccine. Pediatr Infect Dis J 17:386–390 [CrossRef]
     [Google Scholar]
/content/journal/jgv/10.1099/vir.0.19665-0
Loading
A comparison of the effects of oral inoculation with Rotashield and pentavalent reassortant rotavirus vaccine (WC3-PV) on suckling CB17scid mice
J. Gen. Virol. 85, 2245 (2004); https://doi.org/10.1099/vir.0.19665-0
/content/journal/jgv/10.1099/vir.0.19665-0
/content/journal/jgv/10.1099/vir.0.19665-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error