1887

Abstract

The complete nucleotide sequence of a new insect picorna-like virus, Ectropis obliqua picorna-like virus (EoPV), which causes a fatal infection of larvae, has been determined. The genomic RNA of EoPV is 9394 nt in length and contains a single, large open reading frame (nt 391–9351) encoding a polyprotein of 2987 aa. Sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus RNA helicase, protease and RNA-dependent RNA polymerase proteins are found on the genome in order in the 5′→3′ direction. All structural genes were located at the 5′ terminus. In terms of sequence similarity, identity and genome organization, EoPV resembles mammalian picornaviruses and three other insect picorna-like viruses: of silkworm, of honeybee and (PnPV). Phylogenetic analysis showed that EoPV is most closely related to PnPV and suggests that these four insect picorna-like viruses might constitute a new group of insect-infectious RNA viruses.

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2004-05-01
2024-10-10
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment tool. J Mol Biol 215:403–410
    [Google Scholar]
  2. Christian P., Carstens E., Domier L., Johnson K., Nakashima N., Scotti P., van der Wilk F. 2000; ‘Cricket paralysis-like viruses’. In Virus Taxonomy . Seventh Report of the International Committee on Taxonomy of Viruses . pp  678–683 Edited by Regenmortel M. H. V. Van, Fauquet C. M., Bishop D. H. L., Calisher C. H., Carsten E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B. San Diego, CA: Academic Press;
  3. Czibener C., Torre J. L., Muscio O. A., Ugalde R. A., Scodeller E. A. 2000; Nucleotide sequence analysis of Triatoma virus shows that it is a member of a novel group of insect RNA viruses. J Gen Virol 81:1149–1154
    [Google Scholar]
  4. Donnelly M. L. L., Gani D., Flint M., Monoghan S., Ryan M. D. 1997; The cleavage activity of aphtho- and cardiovirus 2A proteins. J Gen Virol 78:13–21
    [Google Scholar]
  5. Donnelly M. L. L., Luke G., Mehrotra A., Li X., Hughes L. E., Gani D., Ryan M. D. 2001a; Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’. J Gen Virol 82:1013–1025
    [Google Scholar]
  6. Donnelly M. L. L., Hughes L. E., Luke G., Mendoza H., ten Dam E., Gani D., Ryan M. D. 2001b; The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences. J Gen Virol 82:1027–1041
    [Google Scholar]
  7. Ghosh R. C., Ball B. V., Willcocks M. M., Carter M. J. 1999; The complete nucleotide sequence of honeybee sacbrood virus. J Gen Virol 80:1541–1549
    [Google Scholar]
  8. Glyn S. 1990; Structure, function and evolution of picornaviruses. J Gen Virol 71:2483–2501
    [Google Scholar]
  9. Gorbalenya A. E., Koonin E. V., Lai M. M.-C. 1990; Putative papain-related thiol proteases of positive strand RNA viruses. FEBS Lett 288:201–205
    [Google Scholar]
  10. Govan V. A., Leat N., Allsopp M., Davison S. 2000; Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses. Virology 277:457–463
    [Google Scholar]
  11. Hahn H., Palmenberg A. C. 1996; Mutational analysis of the encephalomyocarditis virus primary cleavage. J Virol 70:6870–6875
    [Google Scholar]
  12. Hellen C. U. T., Krausslich H. G., Wimmer E. 1989; Proteolytic processing of polyproteins in the replication of RNA viruses. Biochemistry 28:9881–9890
    [Google Scholar]
  13. Isawa H., Asano S., Sahara K., Iizuka T., Bando H. 1998; Analysis of genetic information of an insect picorna-like virus, infectious flacherie virus of silkworm: evidence for evolutionary relationships among insect, mammalian and plant picorna(-like) viruses. Arch Virol 143:127–143
    [Google Scholar]
  14. Johnson K. N., Christian P. D. 1998; The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family. J Gen Virol 79:191–203
    [Google Scholar]
  15. Koonin E. V. 1991; The phylogeny of RNA-dependent polymerase of positive-strand RNA viruses. J Gen Virol 72:2197–2206
    [Google Scholar]
  16. Koonin E. V., Dolja V. V. 1993; Evolution and taxonomy of positive strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430
    [Google Scholar]
  17. Leat N., Ball B., Govan V., Davison S. 2000; Analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees. J Gen Virol 81:2111–2119
    [Google Scholar]
  18. Liljas L., Tate J., Lin T., Christian P., Johnson J. E. 2002; Evolutionary and taxonomic implications of conserved structural motifs between picornaviruses and insect picorna-like viruses. Arch Virol 147:59–84
    [Google Scholar]
  19. Mayo M. A. 2002; Virus taxonomy – Houston 2002. Arch Virol 147:1071–1076
    [Google Scholar]
  20. Moon J. S., Domier L. L., McCoppin N. K., D'Ary C. J., Jin H. 1998; Nucleotide sequence analysis shows that Rhopalosiphum padi virus is a member of a novel group of insect-infecting RNA viruses. Virology 243:54–65
    [Google Scholar]
  21. Moore N. F., Reavy B., King L. A. 1985; General characteristics, gene organization and expression of small RNA virus of insects. J Gen Virol 66:647–659
    [Google Scholar]
  22. Nakashima N., Sasaki J., Toriyama S. 1999; Determining the nucleotide sequence and capsid-coding region of Himetobi P virus: a member of a novel group of RNA viruses that infect insects. Arch Virol 144:2051–2058
    [Google Scholar]
  23. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448
    [Google Scholar]
  24. Piccione M. E., Zellner M., Kumosinski T. F., Mason P. W., Grubman M. 1995; Identification of the active-site residues of foot-and-mouth disease virus leader protease. J Virol 69:4950–4956
    [Google Scholar]
  25. Roberts P. J., Belsham G. J. 1995; Identification of critical amino acids within the foot-and-mouth disease virus leader protein, a cysteine protease. Virology 213:140–146
    [Google Scholar]
  26. Sasaki J., Nakashima N., Saito H., Noda H. 1998; An insect picorna-like virus, Plautia stali intestine virus, has genes of capsid proteins in its 3′ part of the genome. Virology 244:50–58
    [Google Scholar]
  27. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  28. Vadim I. A., Aniko V. P., Eckard W. 1999; Paradoxes of the replication of picornaviral genomes. Virus Res 62:129–147
    [Google Scholar]
  29. van Munster M., Dullemans A. M., Verbeek M., van den Heuvel J. F. J. M., Clerivet A., van der Wilk F. 2002; Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae . J Gen Virol 83:3131–3138
    [Google Scholar]
  30. Wang X.-C., Zhang J.-M., Liu C.-F., Hu Y.-Y. 2004; A new insect picornavirus isolated from Ectropis oblique . Virol Sinica 19:39–43
    [Google Scholar]
  31. Wilson J. E., Powell M. J., Hoover S. E., Sarnow P. 2000; Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites. Mol Cell Biol 20:4990–4999
    [Google Scholar]
  32. Wu C.-Y., Lo C.-F., Huang C.-J., Yu H.-T., Wang C.-H. 2002; The complete sequence of Perina nuda picorna-like virus, an insect-infecting RNA virus with a genome organization similar to that of the mammalian picornaviruses. Virology 294:312–323
    [Google Scholar]
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