1887

Abstract

A genomic characterization of hepatitis B virus (HBV) was done for 56 pre-S1/pre-S2 genes and 10 full-length HBV genotype C isolates from five Asian countries. Phylogenetic analysis of the pre-S1/pre-S2 genes revealed two major groups within genotype C: one for isolates from southeast Asia including Vietnam, Myanmar and Thailand (named HBV/C1) and the other for isolates from Far East Asia including Japan, Korea and China (named HBV/C2). This finding was confirmed by phylogenetic analysis based on the full-length sequence of 32 HBV genotype C isolates, including 22 from database entries. Two isolates from Okinawa, the island off the southern end of Japan, formed a different branch. Specific amino acid sequence changes were identified in the large S protein (amino acids 51, 54, 60, 62 and 73) and P protein (amino acids 231, 233, 236, 248, 252 and 304). Our results indicate that genotype C of HBV can be classified into at least two subgroups.

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2004-02-01
2024-04-19
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References

  1. Alestig E., Hannoun C., Horal P., Lindh M. 2001a; Hepatitis B virus genotypes in Mongols and Australian Aborigines. Arch Virol 146:2321–2329
    [Google Scholar]
  2. Alestig E., Hannoun C., Horal P., Lindh M. 2001b; Phylogenetic origin of hepatitis B virus strains with precore C-1858 variant. J Clin Microbiol 39:3200–3203
    [Google Scholar]
  3. Asahina Y., Enomoto N., Ogura Y., Kurosaki M., Sakuma I., Izumi N., Marumo F., Sato C. 1996; Sequential changes in full-length genomes of hepatitis B virus accompanying acute exacerbation of chronic hepatitis B. J Hepatol 25:787–794
    [Google Scholar]
  4. Bock C. T., Tillmann H. L., Manns M. P., Trautwein C. 1999; The pre-S region determines the intracellular localization and appearance of hepatitis B virus. Hepatology 30:517–525
    [Google Scholar]
  5. Bowyer S. M., van Staden L., Kew M. C., Sim J. G. 1997; A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa. J Gen Virol 78:1719–1729
    [Google Scholar]
  6. Cui C., Shi J., Hui L., Xi H., Zhuoma, Quni Tsedan., Hu G. 2002; The dominant hepatitis B virus genotype identified in Tibet is a C/D hybrid. J Gen Virol 83:2773–2777
    [Google Scholar]
  7. Horikita M., Itoh S., Yamamoto K., Shibayama T., Tsuda F., Okamoto H. 1994; Differences in the entire nucleotide sequence between hepatitis B virus genomes from carriers positive for antibody to hepatitis B e antigen with and without active disease. J Med Virol 44:96–103
    [Google Scholar]
  8. Kao J. H., Chen P. J., Lai M. Y., Chen D. S. 2000; Hepatitis B genotypes correlate with clinical outcomes in patients with chronic hepatitis B. Gastroenterology 118:554–559
    [Google Scholar]
  9. Kao J. H., Chen P. J., Lai M. Y., Chen D. S. 2002; Genotypes and clinical phenotypes of hepatitis B virus in patients with chronic hepatitis B virus infection. J Clin Microbiol 40:1207–1209
    [Google Scholar]
  10. Kidd-Ljunggren K., Miyakawa Y., Kidd A. H. 2002; Genetic variability in hepatitis B viruses. J Gen Virol 83:1267–1280
    [Google Scholar]
  11. Kramvis A., Weitzmann L., Owiredu W. K., Kew M. C. 2002; Analysis of the complete genome of subgroup A′ hepatitis B virus isolates from South Africa. J Gen Virol 83:835–839
    [Google Scholar]
  12. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245
    [Google Scholar]
  13. Lee W. M. 1997; Hepatitis B virus infection. N Engl J Med 337:1733–1745
    [Google Scholar]
  14. Lin X., Qian G. S., Lu P. X., Wu L., Wen Y. M. 2001; Full-length genomic analysis of hepatitis B virus isolates in a patient progressing from hepatitis to hepatocellular carcinoma. J Med Virol 64:299–304
    [Google Scholar]
  15. Lindh M., Andersson A. S., Gusdal A. 1997; Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus – large-scale analysis using a new genotyping method. J Infect Dis 175:1285–1293
    [Google Scholar]
  16. Lok A. S. 2000; Hepatitis B infection: pathogenesis and management. J Hepatol 32:89–97
    [Google Scholar]
  17. Magnius L. O., Norder H. 1995; Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 38:24–34
    [Google Scholar]
  18. Melegari M., Bruno S., Wands J. R. 1994; Properties of hepatitis B virus pre-S1 deletion mutants. Virology 199:292–300
    [Google Scholar]
  19. Morozov V., Pisareva M., Groudinin M. 2000; Homologous recombination between different genotypes of hepatitis B virus. Gene 260:55–65
    [Google Scholar]
  20. Naito H., Hayashi S., Abe K. 2001; Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers. J Clin Microbiol 39:362–364
    [Google Scholar]
  21. Neurath A. R., Kent S. B., Strick N., Parker K. 1986; Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:429–436
    [Google Scholar]
  22. Norder H., Courouce A. M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503
    [Google Scholar]
  23. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R. I., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 69:2575–2583
    [Google Scholar]
  24. Orito E., Mizokami M., Ina Y., Moriyama E. N., Kameshima N., Yamamoto M., Gojobori T. 1989; Host-independent evolution and a genetic classification of the hepadnavirus family based on nucleotide sequences. Proc Natl Acad Sci U S A 86:7059–7062
    [Google Scholar]
  25. Orito E., Mizokami M., Sakugawa H., Michitaka K., Ishikawa K., Ichida T., Okanoue T., Yotsuyanagi H., Iino S. 2001; A case–control study for clinical and molecular biological differences between hepatitis B viruses of genotypes B and C. Japan HBV Genotype Research Group. Hepatology 33:218–223
    [Google Scholar]
  26. Rho H. M., Kim K., Hyun S. W., Kim Y. S. 1989; The nucleotide sequence and reading frames of a mutant hepatitis B virus subtype adr. Nucleic Acids Res 17:2124
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Shiina S., Fujino H., Uta Y. 7 other authors 1991; Relationship of HBsAg subtypes with HBeAg/anti-HBe status and chronic liver disease. Part I: analysis of 1744 HBsAg carriers. Am J Gastroenterol 86:866–871
    [Google Scholar]
  29. Sugauchi F., Mizokami M., Orito E. 7 other authors 2001; A novel variant genotype C of hepatitis B virus identified in isolates from Australian Aborigines: complete genome sequence and phylogenetic relatedness. J Gen Virol 82:883–892
    [Google Scholar]
  30. Sugauchi F., Orito E., Ichida T. & 9 other authors (2002a). Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 76:5985–5992
    [Google Scholar]
  31. Sugauchi F., Chutaputti A., Orito E., Kato H., Suzuki S., Ueda R., Mizokami M. 2002b; Hepatitis B virus genotypes and clinical manifestation among hepatitis B carriers in Thailand. J Gastroenterol Hepatol 17:671–676
    [Google Scholar]
  32. Sugauchi F., Orito E., Ichida T. 10 other authors 2003; Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C. Gastroenterology 124:925–932
    [Google Scholar]
  33. Yuasa R., Takahashi K., Dien B. V., Binh N. H., Morishita T., Sato K., Yamamoto N., Isomura S., Yoshioka K., Ishikawa T., Mishiro S., Kakumu S. 2000; Properties of hepatitis B virus genome recovered from Vietnamese patients with fulminant hepatitis in comparison with those of acute hepatitis. J Med Virol 61:23–28
    [Google Scholar]
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