1887

Abstract

The gene ( infectivity factor) of nucleopolyhedrovirus (SpliNPV) encodes a structural protein essential for oral infection. This protein is expressed in very low quantities. In this study, transcription and promoter analysis of SpliNPV were carried out to understand more fully the regulation of gene expression. Transcription in the gene region was examined using RT-PCR, Northern blot, primer extension, ribonuclease protection and 3′ RACE. The gene was encoded by a late bicistronic messenger, which was characterized. This 1·9 kb messenger was present in very small amounts. In addition, this messenger was part of a set of six late mRNAs overlapping the sequence. A functional complementation assay was used to analyse the promoter. This assay allowed the detection of amounts of PIF which were sufficient for the production of orally infectious virions. The 13 bp region upstream from the initial ATG of was required and sufficient for the production of orally infectious virions. This promoter region was much shorter than the studied baculovirus promoters. A late promoter motif (TTAAG) is situated at the 5′ end of this region. This motif was shown to be the promoter core by using single mutations of the motif in the complementation assay. These results suggest that the low expression of the gene is regulated chiefly at the transcriptional level.

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2004-02-01
2019-11-14
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