1887

Abstract

multicapsid nucleopolyhedrovirus (MNPV) is one of the most successful biological insecticides. In this study, we cloned and sequenced a 12·5 kbp HI-D restriction endonuclease fragment of the MNPV isolate 2D genome that includes the gene. We compared this highly conserved region with that of other baculoviruses. MNPV contained two genes, and , in common with MNPV (MNPV) that were not present in other baculoviruses. The gene has homology to a eukaryotic 3′ repair exonuclease and appears to have been acquired from an invertebrate host. The gene product has the potential to be involved in virus recombination or UV-light tolerance. Multigene phylogenetic analysis suggested that MNPV is most closely related to MNPV (MNPV). MNPV differed from other group I NPVs in that and gene homologues were missing from the region downstream of the gene. Proteinase assays and genetic probes suggest the gene is absent from MNPV.

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2004-01-01
2020-01-20
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