1887

Abstract

multicapsid nucleopolyhedrovirus (MNPV) is one of the most successful biological insecticides. In this study, we cloned and sequenced a 12·5 kbp HI-D restriction endonuclease fragment of the MNPV isolate 2D genome that includes the gene. We compared this highly conserved region with that of other baculoviruses. MNPV contained two genes, and , in common with MNPV (MNPV) that were not present in other baculoviruses. The gene has homology to a eukaryotic 3′ repair exonuclease and appears to have been acquired from an invertebrate host. The gene product has the potential to be involved in virus recombination or UV-light tolerance. Multigene phylogenetic analysis suggested that MNPV is most closely related to MNPV (MNPV). MNPV differed from other group I NPVs in that and gene homologues were missing from the region downstream of the gene. Proteinase assays and genetic probes suggest the gene is absent from MNPV.

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2004-01-01
2024-04-24
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References

  1. Afonso C. L., Tulman E. R., Lu Z., Balinsky C. A., Moser B. A., Becnel J. J., Rock D. L., Kutish G. F. 2001; Genome sequence of a baculovirus pathogenic for Culex nigripalpus . J Virol 75:11157–11165
    [Google Scholar]
  2. Bernad A., Blanco L., Lazaro J. M., Martin G., Salas M. 1989; A conserved 3′–5′ exonuclease active site in prokaryotic and eukaryotic DNA polymerases. Cell 59:219–228
    [Google Scholar]
  3. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
    [Google Scholar]
  4. Bulach D. M., Kumar C. A., Zaia A., Liang B., Tribe D. E. 1999; Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. J Invertebr Pathol 73:59–73
    [Google Scholar]
  5. Croizier G., Ribeiro H. C. T. 1992; Recombination as a possible major cause of genetic heterogeneity in Anticarsia gemmatalis nuclear polyhedrosis virus. Virus Res 26:183–196
    [Google Scholar]
  6. Dai X., Hajos J. P., Joosten N. N., van Oers M. M., IJkel W. F. J., Zuidema D., Pang Y., Vlak J. M. 2000; Isolation of a Spodoptera exigua baculovirus recombinant with a 10·6 kbp genome deletion that retains biological activity. J Gen Virol 81:2545–2554
    [Google Scholar]
  7. Garcia-Maruniak A., Pavan O. H. O., Maruniak J. E. 1996; A variable region of Anticarsia gemmatalis nuclear polyhedrosis virus contains tandemly repeated DNA sequences. Virus Res 41:123–132
    [Google Scholar]
  8. Guarino L. A., Gonzalez M. A., Summers M. D. 1986; Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. J Virol 60:224–229
    [Google Scholar]
  9. Hashimoto Y., Hayakawa T., Ueno Y., Fujita T., Sano Y., Matsumoto T. 2000; Sequence analysis of the Plutella xylostella granulovirus genome. Virology 275:358–372
    [Google Scholar]
  10. Hawtin R. E., Zarkowska T., Arnold K., Thomas C. J., Gooday G. W., King L. A., Kuzio J. A., Possee R. D. 1997; Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology 238:243–253
    [Google Scholar]
  11. Herniou E. A., Luque T., Chen X., Vlak J. M., Winstanley D., Cory J. S., O'Reilly D. R. 2001; Use of whole genome sequence data to infer baculovirus phylogeny. J Virol 75:81117–81126
    [Google Scholar]
  12. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature 226:466–467
    [Google Scholar]
  13. Hom L. G., Volkman L. E. 2000; Autographa californica M nucleopolyhedrovirus chiA is required for processing of V-CATH. Virology 277:178–183
    [Google Scholar]
  14. Inceoglu A. B., Kamita S. G., Hinton A. C., Huang Q., Severson T. F., Kang K.-D., Hammock B. D. 2001; Recombinant baculoviruses for insect control. Pest Man Sci 57:981–987
    [Google Scholar]
  15. Johnson D. W., Maruniak J. E. 1989; Physical map of Anticarsia gemmatalis nuclear polyhedrosis virus AgMNPV-2 DNA. J Gen Virol 70:1877–1884
    [Google Scholar]
  16. Katsuma S., Noguchi Y., Zhou C. L., Kobayashi M., Maeda S. 1999; Characterization of the 25K FP gene of the baculovirus Bombyx mori nucleopolyhedrovirus: implications for post-mortem host degradation. J Gen Virol 80:783–791
    [Google Scholar]
  17. Kool M., Van Den Berg P. M. M. M., Tramper J., Goldbach R. W., Vlak J. M. 1993; Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology 192:94–101
    [Google Scholar]
  18. Kool M., Goldbach R. W., Vlak J. M. 1994; A putative non- hr origin of DNA replication in the Hin dIII-K fragment of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus. J Gen Virol 75:3345–3352
    [Google Scholar]
  19. Koonin E. V., Deutscher M. P. 1993; RNase T shares conserved sequence motifs with DNA proofreading exonucleases. Nucleic Acids Res 21:2521–2522
    [Google Scholar]
  20. Maruniak J. E., Garcia-Maruniak A., Souza M. L., Zanotto P. M., Moscardi F. 1999; Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants. Arch Virol 144:1991–2006
    [Google Scholar]
  21. Mazur D. J., Perrino F. W. 1999; Identification and expression of TREX1 and TREX2 cDNA sequences encoding mammalian 3′–5′ exonucleases. J Biol Chem 274:19655–19660
    [Google Scholar]
  22. Mazur D. J., Perrino F. W. 2001; Excision of 3′ termini by Trex1 and TREX2 3′–5′ exonucleases. J Biol Chem 276:17022–17029
    [Google Scholar]
  23. Moscardi F. 1999; Assessment of the application of baculoviruses for control of lepidoptera. Annu Rev Entomol 44:257–289
    [Google Scholar]
  24. O'Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors: a Laboratory Manual New York: W. H. Freeman;
    [Google Scholar]
  25. Pearson M. N., Rohrmann G. F. 1995; Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. J Virol 69:213–221
    [Google Scholar]
  26. Pilloff M. G., Bilen M. F., Belaich M. N., Lozano M. E., Ghiringhelli P. D. 2003; Molecular cloning and sequence analysis of the Anticarsia gemmatalis multicapsid nuclear polyhedrosis virus GP64 glycoprotein. Virus Genes 26:57–69
    [Google Scholar]
  27. Razuck F. B., Ribeiro B., Vargas J. H., Wolff J. L., Ribeiro B. M. 2002; Characterization of the p10 gene region of Anticarsia gemmatalis nucleopolyhedrovirus. Virus Genes 24:243–247
    [Google Scholar]
  28. Rodrigues J. C., De Souza M. L., O'Reilly D., Velloso L. M., Pinedo F. J., Razuck F. B., Ribeiro B., Ribeiro B. M. 2001; Characterization of the ecdysteroid UDP-glucosyltransferase ( egt ) gene of Anticarsia gemmatalis nucleopolyhedrovirus. Virus Genes 22:103–112
    [Google Scholar]
  29. Slack J. M., Kuzio J., Faulkner P. 1995; Characterization of v- cath , a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus. J Gen Virol 76:1091–1098
    [Google Scholar]
  30. Swofford D. L. 1998 paup*. Phylogenetic Analysis Using Parsimony (*and Other Methods), version 4.0 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  31. Theilmann D. A., Stewart S. 1992; Tandemly repeated sequence at the 3′ end of the IE-2 gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element. Virology 187:97–106
    [Google Scholar]
  32. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  33. Zanotto P. M. D. A., Sampaio M. J., Johnson D. W., Rocha T. L., Maruniak J. E. 1992; The Anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region sequence analysis gene product and structural comparisons. J Gen Virol 73:1049–1056
    [Google Scholar]
  34. Zanotto P. M., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. J Invertebr Pathol 62:147–164
    [Google Scholar]
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