1887

Abstract

Endemic circulation of hepatitis E virus (HEV) in Namibia was suspected from serological data during an outbreak of non-A, non-B hepatitis in Rundu in 1995. The source of the outbreak was suspected to be the water supply, which had been compromised approximately 6 months earlier. Four HEV isolates from four different persons in this outbreak were successfully amplified, sequenced and analysed over a 451 bp region of a subgenomic fragment from the 3′ end of the genome in ORF2. Phylogenetic analysis showed that the four Namibian HEV isolates clustered with a Mexican isolate in genotype II and shared 85·8–86·3 % nucleotide identity with the 1987 Mexican isolate, but were only 77·6–79·6 % similar to other African isolates. HEV isolated from the same region of Namibia in 1983 was reported to cluster in genotype I. However, virus isolates from sporadic cases of HEV isolated in 1997/8 in Nigeria were also from genotype II.

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2004-01-01
2019-10-18
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References

  1. Benjelloun, S., Bahbouhi, B., Bouchrit, N., Hda, N., Mahjour, J. & Benslimane, A. ( 1997; ). Seroepidemiological study of an acute hepatitis E outbreak in Morocco. Res Virol 148, 279–287.[CrossRef]
    [Google Scholar]
  2. Bradley, D. W. ( 1992; ). Hepatitis E: epidemiology, aetiology and molecular biology. Rev Med Virol 2, 19–28.[CrossRef]
    [Google Scholar]
  3. Buisson, Y., Grandadam, M., Nicand, E. & 7 other authors ( 2000; ). Identification of a novel hepatitis E virus in Nigeria. J Gen Virol 81, 903–909.
    [Google Scholar]
  4. Byskov, J., Wouters, J. S. M., Sathekge, T. J. & Swanepoel, R. ( 1989; ). An outbreak of suspected water-borne epidemic non-A non-B hepatitis in northern Botswana with a high prevalence of hepatitis B carriers and hepatitis delta markers among patients. Trans R Soc Trop Med Hyg 83, 110–116.[CrossRef]
    [Google Scholar]
  5. Coursaget, R., Buisson, Y., Enogat, N. & 8 other authors ( 1996; ). Hepatitis E virus infections in France and Africa. In Enterically-Transmitted Hepatitis Viruses, pp. 201–212. Edited by Y. Buisson, P. Coursaget & M. Kane. France: La Simarre, Joue-les-Tours.
  6. Grabow, W. O. K., Taylor, M. B. & Webber, L. M. ( 1996; ). Hepatitis E virus in South Africa. S Afr J Sci 92, 178–180.
    [Google Scholar]
  7. He, J., Binn, L. N., Tsarev, S. A., Hayes, C. G., Frean, J. A., Isaacson, M. & Innis, B. L. ( 2000; ). Molecular characterization of a hepatitis E virus isolate from Namibia. J Biomed Sci 7, 334–338.[CrossRef]
    [Google Scholar]
  8. Hsieh, S. Y., Yang, P. Y., Ho, Y. P., Chu, C. M. & Liaw, Y. F. ( 1998; ). Identification of a novel strain of hepatitis E virus responsible for sporadic acute hepatitis in Taiwan. J Med Virol 55, 300–304.[CrossRef]
    [Google Scholar]
  9. Irshad, M. ( 1999; ). Hepatitis E virus: an update on its molecular, clinical and epidemiological characteristics. Intervirology 42, 252–262.[CrossRef]
    [Google Scholar]
  10. Isaacson, M., Frean, J., He, J., Seriwatana, J. & Innis, B. L. ( 2000; ). An outbreak of hepatitis E in Northern Namibia, 1983. Am J Trop Med Hyg 62, 619–625.
    [Google Scholar]
  11. Krawczynski, K., Aggarwal, R. & Kamili, S. ( 2000; ). Hepatitis E. Infect Dis Clin North Am 14, 669–687.[CrossRef]
    [Google Scholar]
  12. Mast, E., Polish, L., Favorov, M. & 8 other authors ( 1994; ). Hepatitis E among refugees in Kenya: apparent person to person transmission, evidence for age-dependent disease expression and new serological assays. In Viral Hepatitis and Liver Disease, 1994, pp. 375–378. Edited by K. Nishioka, H. Suzuki, S. Mishiro & T. Oda. Tokyo: Springer.
  13. Muerhoff, A. S., Smith, D. B., Leary, T. P., Erker, J. C., Desai, S. M. & Mushahwar, I. K. ( 1997; ). Identification of GB virus C variants by phylogenetic analysis of 5′-untranslated and coding region sequences. J Virol 71, 6501–6508.
    [Google Scholar]
  14. Purcell, R. H. ( 1996; ). Hepatitis E virus. In Fields Virology, 3rd edn, vol. 2, pp. 2831–2843. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  15. Schlauder, G. G., Dawson, G. J., Erker, J. C., Kwo, P. Y., Knigge, M. F., Smalley, D. L., Rosenblatt, J. E., Desai, S. M. & Mushahwar, I. K. ( 1998; ). The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States. J Gen Virol 79, 447–456.
    [Google Scholar]
  16. Schlauder, G. G., Frider, B., Sookoian, S., Castano, G. C. & Mushahwar, I. K. ( 2000; ). Identification of 2 novel isolates of hepatitis E virus in Argentina. J Infec Dis 182, 294–297.[CrossRef]
    [Google Scholar]
  17. Surveillance Bulletin, January ( 1996; ). Hepatitis, NIV. South African Virus Laboratories, p. 11.
  18. Takahashi, M., Nishizawa, T., Yoshikawa, A., Sato, S., Isoda, N., Ido, K., Sugano, K. & Okamoto, H. ( 2002; ). Identification of two distinct genotypes of hepatitis E virus in a Japanese patient with acute hepatitis who had not travelled abroad. J Gen Virol 83, 1931–1940.
    [Google Scholar]
  19. Tassopoulos, N. C., Krawczynski, K., Hatzakis, A., Katsoulidou, A., Delladetsima, I., Koutelou, M. G. & Trichopoulos, D. ( 1994; ). Case report: role of hepatitis E virus in the etiology of community-acquired non-A, non-B hepatitis in Greece. J Med Virol 42, 124–128.[CrossRef]
    [Google Scholar]
  20. Tsarev, S. A., Binn, L. N., Gomatos, P. J., Arthur, R. R., Monier, M. K., van Cuyck-Gandre, H., Longer, C. F. & Innis, B. L. ( 1999; ). Phylogenetic analysis of hepatitis E virus isolates from Egypt. J Med Virol 57, 68–74.[CrossRef]
    [Google Scholar]
  21. Tsega, E., Krawczynski, K., Hansson, B. G., Nordenfelt, E., Negusse, Y., Alemu, W. & Bahru, Y. ( 1991; ). Outbreak of acute hepatitis E virus infection among military personnel in Northern Ethiopia. J Med Virol 34, 232–236.[CrossRef]
    [Google Scholar]
  22. Tucker, T. J., Kirsch, R. E., Louw, S. J., Isaacs, S., Kannemeyer, J. & Robson, S. C. ( 1996; ). Hepatitis E in South Africa: evidence for sporadic spread and increased seroprevalence in rural areas. J Med Virol 50, 117–119.[CrossRef]
    [Google Scholar]
  23. van Cuyck-Gandre, H., Zhang, H. Y., Clements, N. J., Cohen, S. G., Caudill, J. D., Coursaget, P., Buisson, Y., Warren, R. L. & Longer, C. F. ( 1996; ). Partial sequence of HEV isolates from North Africa and comparison with known HEV sequences. In Enterically-Transmitted Hepatitis Viruses: Proceedings of the International Symposium on Enterically-Transmitted Hepatitis Viruses (1995: Paris, France), pp. 301–310. Edited by Y. Buisson, P. Coursaget & M. Kane. France: La Simarre, Joue-les-Tours.
  24. van Cuyck-Gandre, H., Zhang, H. Y., Tsarev, S. A., Warren, R. L., Caudill, J. D., Snellings, N. J., Begot, L., Innis, B. L. & Longer, F. ( 2000; ). Short report: phylogenetically distinct hepatitis E viruses in Pakistan. Am J Trop Med Hyg 62, 187–189.
    [Google Scholar]
  25. van Rensburg, E. J., Lemmer, H. R. & Joubert, J. J. ( 1995; ). Prevalence of viral infections in Mozambican refugees in Swaziland. East Afr Med J 72, 588–590.
    [Google Scholar]
  26. Wang, Y., Zhang, H., Ling, R., Li, H. & Harrison, T. J. ( 2000; ). The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J Gen Virol 81, 1675–1686.
    [Google Scholar]
  27. Widdowson, M. A., Jaspers, W. J., van der Poel, W. H. M., Verschoor, F., de Roda Husman, A. M., Winter, H. L. J., Zaaijer, H. L. & Koopmans, M. ( 2003; ). Cluster of cases of acute hepatitis associated with hepatitis E virus infection acquired in The Netherlands. Clin Infect Dis 36, 29–33.[CrossRef]
    [Google Scholar]
  28. Worm, H. C., Schlauder, G. G., Wurzer, H. & Mushahwar, I. K. ( 2000; ). Identification of a novel variant of hepatitis E virus in Austria: sequence, phylogenetic and serological analysis. J Gen Virol 81, 2885–2890.
    [Google Scholar]
  29. Zaaijer, H. L., Kok, M., Lelie, P. N., Timmerman, R. J., Chau, K. & van der Pal, H. J. H. ( 1993; ). Hepatitis E in The Netherlands: imported and endemic. Lancet 341, 826.[CrossRef]
    [Google Scholar]
  30. Zanetti, A. R., Dawson, G. J. & The Study Group of Hepatitis E ( 1994; ). Hepatitis type E in Italy: a seroepidemiological survey. J Med Virol 42, 318–320.[CrossRef]
    [Google Scholar]
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