Human T-cell leukaemia virus type I is highly sensitive to UV-C light Free

Abstract

The biological characteristics of human T-cell leukaemia virus type I (HTLV-I) are not yet well understood. UV light C (UV-C) sensitivity of HTLV-I was studied using a newly established infectivity assay: infection with cell-free HTLV-I dose-dependently induced syncytial plaques in cat cells transduced with the gene of HTLV-I. HTLV-I was inactivated by a much lower UV dose than bovine leukaemia virus (BLV). The D (10 % survival dose) of HTLV-I was about 20 J m, while that of BLV was about 180 J m, which was similar to the reported D of BLV. The UV sensitivity of HTLV-I and BLV was also examined by detecting viral DNA synthesis 24 h after infection. The D values determined by PCR using the primers for HTLV-I and BLV were close to those determined by the infectivity assays. Further PCR analyses were then performed to determine D values using several different primers located between the 5′-long terminal repeat (5′-LTR) and the gene. The difference in UV sensitivity between HTLV-I and BLV was detected very early during replication, even during reverse transcription of the 5′-LTR of irradiated viruses, and became more prominent as reverse transcription proceeded towards the gene. Chimeric mouse retroviruses that contain the LTR- fragments of HTLV-I and BLV were made and hardly any difference in UV sensitivity was detected between them, suggesting that the difference was not determined by the linear RNA sequences of HTLV-I and BLV. HTLV-I was found to be much more sensitive than other retroviruses to UV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19578-0
2004-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/8/vir852397.html?itemId=/content/journal/jgv/10.1099/vir.0.19578-0&mimeType=html&fmt=ahah

References

  1. Akagi T., Nyunoya H., Shimotohno K. 1991; Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1. Gene 106:255–259 [CrossRef]
    [Google Scholar]
  2. Bogdanov K. V., Chukhlovin A. B., Zaritskey A. Y., Frolova O. I., Afanasiev B. V. 1997; Ultraviolet irradiation induces multiple DNA double-strand breaks and apoptosis in normal granulocytes and chronic myeloid leukemia blasts. Br J Haematol 98:869–872 [CrossRef]
    [Google Scholar]
  3. Clapham P., Nagy K., Weiss R. A. 1984; Pseudotypes of human T-cell leukemia virus types 1 and 2: neutralization by patients' sera. Proc Natl Acad Sci U S A 81:2886–2889 [CrossRef]
    [Google Scholar]
  4. Fan N., Gavalchin J., Paul B., Wells K. H., Lane M. J., Poiesz B. J. 1992; Infection of peripheral blood mononuclear cells and cell lines by cell-free human T-cell lymphoma/leukemia virus type I. J Clin Microbiol 30:905–910
    [Google Scholar]
  5. Fischinger P. J., Peebles P. T., Nomura S., Haapala D. K. 1973; Isolation of RD-114-like oncornavirus from a cat cell line. J Virol 11:978–985
    [Google Scholar]
  6. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., de The G. 1985; Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet 24:407–410
    [Google Scholar]
  7. Guillemain B., Mamoun R., Astier T., Duplan J. F. 1981; Mechanism of early and late polykaryocytosis induced by the bovine leukaemia virus. J Gen Virol 57:227–231 [CrossRef]
    [Google Scholar]
  8. Haapala D. K., Robey W. G., Oroszlan S. D., Tsai W. P. 1985; Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J Virol 53:827–833
    [Google Scholar]
  9. Haraguchi Y., Yang D. W., Handa A., Shimizu N., Tanaka Y., Hoshino H. 1994; Detection of neutralizing antibodies against human T-cell leukemia virus type 1 using a cell-free infection system and polymerase chain reaction. Int J Cancer 59:416–421 [CrossRef]
    [Google Scholar]
  10. Henderson E. E., Tudor G., Yang J. Y. 1992; Inactivation of the human immunodeficiency virus type 1 (HIV-1) by ultraviolet and X irradiation. Radiat Res 131:169–176 [CrossRef]
    [Google Scholar]
  11. Hinuma Y., Nagata K., Nanaoka M., Nakai M., Matsumoto T., Kinoshita K. I., Shirakawa S., Miyoshi I. 1981; Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sci U S A 78:6476–6480 [CrossRef]
    [Google Scholar]
  12. Hoshino H., Shimoyama M., Miwa M., Sugimura T. 1983; Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay. Proc Natl Acad Sci U S A 80:7337–7341 [CrossRef]
    [Google Scholar]
  13. Hoshino H., Clapham P. R., Weiss R. A., Miyoshi I., Yoshida M., Miwa M. 1985; Human T-cell leukemia virus type I: pseudotype neutralization of Japanese and American isolates with human and rabbit sera. Int J Cancer 36:671–675 [CrossRef]
    [Google Scholar]
  14. Hoshino H., Nakamura T., Tanaka Y., Miyoshi I., Yanagihara R. 1993; Functional conservation of the neutralizing domains on the external envelope glycoprotein of cosmopolitan and melanesian strains of human T-cell leukemia/lymphoma virus type I. J Infect Dis 168:1368–1373 [CrossRef]
    [Google Scholar]
  15. Jacobson S., Raine C. S., Mingioli E. S., McFarlin D. E. 1988; Isolation of an HTLV-1-like retrovirus from patients with tropical spastic paraparesis. Nature 331:540–543 [CrossRef]
    [Google Scholar]
  16. Levinson W., Rubin H. 1966; Radiation studies of avian tumor viruses and of Newcastle disease virus. Virology 28:533–542 [CrossRef]
    [Google Scholar]
  17. Lovinger G. G., Ling H. P., Gilden R. V., Hatanaka M. 1975; Effect of UV light on RNA-directed DNA polymerase activity of murine oncornaviruses. J Virol 15:1273–1275
    [Google Scholar]
  18. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. 1981; Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature 294:770–771 [CrossRef]
    [Google Scholar]
  19. Miyoshi I., Yoshimoto S., Taguchi H., Kubonishi I., Fujishita M., Ohtsuki Y., Shiraishi Y., Akagi T. 1983; Transformation of rabbit lymphocytes with T-cell leukemia virus. Gann 74:1–4
    [Google Scholar]
  20. Morcock D. R., Katakam S., Kane B. P., Casas-Finet J. R. 2002; Fluorescence and nucleic acid binding properties of bovine leukemia virus nucleocapsid protein. Biophys Chem 97:203–212 [CrossRef]
    [Google Scholar]
  21. Murphy T. M., Gordon M. P. 1981; Photobiology of RNA viruses. In Comprehensive Virology , Methods Used in the Study of Viruses . vol 17 pp  285–349 Edited by Fraenkel-Conrat H., Wangeer R. New York: Plenum;
  22. Nagy K., Clapham P., Cheingsong-Popov R., Weiss R. A. 1983; Human T-cell leukemia virus type I: induction by syncytia and inhibition by patients' sera. Int J Cancer 32:321–328 [CrossRef]
    [Google Scholar]
  23. Osame M., Usuku K., Izumo S., Ijuchi N., Amitani H., Igata A., Matsumoto M., Tara M. 1986; HTLV-I associated myelopathy, a new clinical entity. Lancet 3:1031–1032
    [Google Scholar]
  24. Owada M., Ihara S., Toyoshima K., Sugino Y., Kozai Y. 1976; Ultraviolet inactivation of avian sarcoma viruses: biological and biochemical analysis. Virology 69:710–718 [CrossRef]
    [Google Scholar]
  25. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A 77:7415–7419 [CrossRef]
    [Google Scholar]
  26. Sagata N., Yasunaga T., Ogawa Y., Tsuzuku-Kawamura J., Ikawa Y. 1984; Bovine leukemia virus: unique structural features of its long terminal repeats and its evolutionary relationship to human T-cell leukemia virus. Proc Natl Acad Sci U S A 81:4741–4745 [CrossRef]
    [Google Scholar]
  27. Soda Y., Shimizu N., Jinno A., Liu H. Y., Kanbe K., Kitamura T., Hoshino H. 1999; Establishment of a new system for determination of coreceptor usages of HIV based on the human glioma NP-2 cell line. Biochem Biophys Res Commun 258:313–321 [CrossRef]
    [Google Scholar]
  28. Telesnitsky A., Goff S. P. 1997; Reverse transcriptase and the generation of retroviral DNA. In Retroviruses pp  144–145 Edited by Coffin J. M., Hughes S. H., Varmus H. E. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Van der Maaten M. J., Miller J. M. 1975; Replication of bovine leukemia virus in monolayer cell cultures. Bibl Haematol 43:360–362
    [Google Scholar]
  30. Yanagihara R., Nerukar V. R., Ajdukiewicz A. B. 1991; Comparison between strains of human T lymphotropic virus type I isolated from inhabitants of the Solomon Islands and Papua New Guinea. J Infect Dis 164:443–449 [CrossRef]
    [Google Scholar]
  31. Yoshikura H. 1989; Thermostability of human immunodeficiency virus (HIV-1) in a liquid matrix is far higher than that of an ecotropic murine leukemia virus. Jpn J Cancer Res 80:1–5 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19578-0
Loading
/content/journal/jgv/10.1099/vir.0.19578-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed