1887

Abstract

Equine herpesvirus-1 (EHV-1) downregulates surface expression of major histocompatibility complex (MHC) class I molecules on infected cells. The objective of this study was to investigate whether EHV-1 interferes with peptide translocation by the transporter associated with antigen processing (TAP) and to identify the proteins responsible. Using an transport assay, we showed that EHV-1 inhibited transport of peptides by TAP as early as 2 h post-infection (p.i). Complete shutdown of peptide transport was observed by 8 h p.i. Furthermore, pulse–chase experiments revealed that maturation of class I molecules in the endoplasmic reticulum (ER) was delayed in EHV-1-infected cells, which may be due to reduced availability of peptides in the ER as a result of TAP inhibition. Metabolic inhibition studies indicated that an early protein(s) of EHV-1 is responsible for this effect.

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2004-02-01
2024-04-19
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References

  1. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Prog Vet Microbiol Immunol 2:78–144
    [Google Scholar]
  2. Allen G., Yeargan M., Costa L. R., Cross R. 1995; Major histocompatibility complex class I-restricted cytotoxic T-lymphocyte responses in horses infected with equine herpesvirus 1. J Virol 69:606–612
    [Google Scholar]
  3. Ambagala A. P., Hinkley S., Srikumaran S. 2000; An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP. J Immunol 164:93–99
    [Google Scholar]
  4. Bryans J. T. 1981; Application of management procedures and prophylactic immunization to the control of equine rhinopneuminitis. Proc Am Assoc Equine Pract 26:259–272
    [Google Scholar]
  5. Bryans J. T., Allen G. P. 1989; Herpesvirus diseases of the horse. In Herpesvirus Diseases of Cattle, Horses and Pigs pp  176–229 Edited by Wittmann G. Boston: Kluwer;
    [Google Scholar]
  6. Burki F., Rossmanith W., Nowotny N., Pallan C., Mostl K., Lussy H. 1990; Viraemia and abortions are not prevented by two commercial equine herpesvirus-1 vaccines after experimental challenge of horses. Vet Q 12:80–86
    [Google Scholar]
  7. Doll E. R., Bryans J. T. 1963; Immunization of young horses against viral rhinopneumonitis. Cornell Vet 53:24–41
    [Google Scholar]
  8. Edington N., Bridges C. G., Huckle A. 1985; Experimental reactivation of equid herpesvirus 1 (EHV 1) following the administration of corticosteroids. Equine Vet J 17:369–372
    [Google Scholar]
  9. Feng X., Thompson Y. G., Lewis J. B., Caughman G. B. 1996; Expression and function of the equine herpesvirus 1 virion-associated host shutoff homolog. J Virol 70:8710–8718
    [Google Scholar]
  10. Gentry M. J., Srikumaran S. 1991; Neutralizing monoclonal antibodies to Pasteurella haemolytica leukotoxin affinity-purify the toxin from crude culture supernatants. Microb Pathog 10:411–417
    [Google Scholar]
  11. Gibson J. S., Slater J. D., Awan A. R., Field H. J. 1992; Pathogenesis of equine herpesvirus-1 in specific pathogen-free foals: primary and secondary infections and reactivation. Arch Virol 123:351–366
    [Google Scholar]
  12. Hannant D., O’Neill T., Ostlund E. N., Kydd J. H., Hopkin P. J., Mumford J. A. 1999; Equid herpesvirus-induced immunosuppression is associated with lymphoid cells and not soluble circulating factors. Viral Immunol 12:313–321
    [Google Scholar]
  13. Hengel H., Koopmann J. O., Flohr T., Muranyi W., Goulmy E., Hammerling G. J., Koszinowski U. H., Momburg F. 1997; A viral ER-resident glycoprotein inactivates the MHC-encoded peptide transporter. Immunity 6:623–632
    [Google Scholar]
  14. Hill A., Jugovic P., York I., Russ G., Bennink J., Yewdell J., Ploegh H., Johnson D. 1995; Herpes simplex virus turns off the TAP to evade host immunity. Nature 375:411–415
    [Google Scholar]
  15. Hinkley S., Hill A. B., Srikumaran S. 1998; Bovine herpesvirus-1 infection affects the peptide transport activity in bovine cells. Virus Res 53:91–96
    [Google Scholar]
  16. Koppers-Lalic D., Rijsewijk F. A., Verschuren S. B., van Gaans-van den Brink J. A., Neisig A., Ressing M. E., Neefjes J., Wiertz E. J. 2001; The UL41-encoded virion host shutoff (vhs) protein and vhs-independent mechanisms are responsible for down-regulation of MHC class I molecules by bovine herpesvirus 1. J Gen Virol 82:2071–2081
    [Google Scholar]
  17. Lehner P. J., Karttunen J. T., Wilkinson G. W., Cresswell P. 1997; The human cytomegalovirus US6 glycoprotein inhibits transporter associated with antigen processing-dependent peptide translocation. Proc Natl Acad Sci U S A 94:6904–6909
    [Google Scholar]
  18. McGuire T. C., Tumas D. B., Byrne K. M., Hines M. T., Leib S. R., Brassfield A. L., O'Rourke K. I., Perryman L. E. 1994; Major histocompatibility complex-restricted CD8+ cytotoxic T lymphocytes from horses with equine infectious anemia virus recognize Env and Gag/PR proteins. J Virol 68:1459–1467
    [Google Scholar]
  19. Martens J. G., Martens R. J. 1991; Equine herpesvirus type 1: its classification, pathogenesis and prevention. Vet Med 86:936–940
    [Google Scholar]
  20. Mumford J. A., Rossdale P. D., Jessett D. M., Gann S. J., Ousey J., Cook R. F. 1987; Serological and virological investigations of an equid herpesvirus 1 (EHV-1) abortion storm on a stud farm in 1985. J Reprod Fertil 35:509–518
    [Google Scholar]
  21. Neefjes J. J., Momburg F., Hammerling G. J. 1993; Selective and ATP-dependent translocation of peptides by the MHC-encoded transporter. Science 261:769–771
    [Google Scholar]
  22. Rappocciolo G., Birch J., Ellis S. A. 2003; Down-regulation of MHC class I expression by equine herpesvirus-1. J Gen Virol 84:293–300
    [Google Scholar]
  23. Tomazin R., Hill A. B., Jugovic P., York I., Van Endert P., Ploegh H. L., Andrews D. W., Johnson D. C. 1996; Stable binding of the herpes simplex virus ICP47 protein to the peptide binding site of TAP. EMBO J 15:3256–3266
    [Google Scholar]
  24. van der Meulen K. M., Nauwynck H. J., Pensaert M. B. 2003; Absence of viral antigens on the surface of equine herpesvirus-1-infected peripheral blood mononuclear cells: a strategy to avoid complement-mediated lysis. J Gen Virol 84:93–97
    [Google Scholar]
  25. Vossen M. T., Westerhout E. M., Soderberg-Naucler C., Wiertz E. J. 2002; Viral immune evasion: a masterpiece of evolution. Immunogenetics 54:527–542
    [Google Scholar]
  26. Williams A., Peh C. A., Elliott T. 2002; The cell biology of MHC class I antigen presentation. Tissue Antigens 59:3–17
    [Google Scholar]
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