1887

Abstract

During adenovirus infection, following capsid dissociation, core protein VII enters the host cell nucleus complexed with adenovirus DNA. In order to determine whether protein VII may have an active role in this nuclear import, regions of the preVII gene were amplified by PCR, and further oligonucleotide mutants were designed with site-directed mutation of codons for the basic amino acids arginine and lysine. Fragments were cloned into a mammalian expression plasmid to express the peptides as N-terminal fusions to enhanced green fluorescent protein. Results demonstrate that preVII protein contains both nuclear and nucleolar targeting sequences. Such signals may be important in the delivery of adenovirus DNA to the host cell nucleus during adenovirus infection. Furthermore, the data suggest that protein VII may bind to human chromosomes by means of two distinct domains, one sharing homology with the N-terminal regulatory tail of histone H3.

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2003-12-01
2019-12-14
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References

  1. Alestrom, P., Akusjärvi, G., Lager, M., Yeh-kai, L. & Pettersson, U. ( 1984; ). Genes encoding the core proteins of adenovirus type 2. J Biol Chem 259, 13980–13985.
    [Google Scholar]
  2. Askham, J. M., Moncur, P., Markham, A. F. & Morrison, E. E. ( 2000; ). Regulation and function of the interaction between the APC tumour suppressor protein and EB1. Oncogene 19, 1950–1958.[CrossRef]
    [Google Scholar]
  3. Black, B. C. & Center, M. S. ( 1979; ). DNA-binding properties of the major core protein of adenovirus 2. Nucleic Acids Res 6, 2339–2353.[CrossRef]
    [Google Scholar]
  4. Cai, F. & Weber, J. M. ( 1993; ). Primary structure of the canine adenovirus PVII protein: functional implications. Virology 193, 986–988.
    [Google Scholar]
  5. Castiglia, C. L. & Flint, S. J. ( 1983; ). Effects of adenovirus infection on rRNA synthesis and maturation in HeLa cells. Mol Cell Biol 3, 662–671.
    [Google Scholar]
  6. Chatterjee, P. K., Vayda, M. E. & Flint, S. J. ( 1985; ). Interactions among the three adenovirus core proteins. J Virol 55, 379–386.
    [Google Scholar]
  7. Chatterjee, P. K., Vayda, M. E. & Flint, S. J. ( 1986a; ). Identification of proteins and protein domains that contact DNA within adenovirus nucleoprotein cores by ultraviolet light crosslinking of oligonucleotides 32P-labelled in vivo. J Mol Biol 188, 23–37.[CrossRef]
    [Google Scholar]
  8. Chatterjee, P. K., Yang, U.-C. & Flint, S. J. ( 1986b; ). Comparison of the interactions of the adenovirus type 2 major core protein and its precursor with DNA. Nucleic Acids Res 14, 2721–2735.[CrossRef]
    [Google Scholar]
  9. Chatterjee, P. K., Vayda, M. E. & Flint, S. J. ( 1986c; ). Adenoviral protein VII packages intracellular viral DNA throughout the early phase of infection. EMBO J 5, 1633–1644.
    [Google Scholar]
  10. Davison, A. J., Benkö, M. & Harrach, B. ( 2003; ). Genetic content and evolution of adenoviruses. J Gen Virol 84, 2895–2908.[CrossRef]
    [Google Scholar]
  11. Farkas, S. L., Benkö, M., Élö, P., Ursu, K., Dán, Á., Ahne, W. & Harrach, B. ( 2002; ). Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake (Elaphe guttata) imply a common origin with members of the proposed new genus Atadenovirus. J Gen Virol 83, 2403–2410.
    [Google Scholar]
  12. Greber, U. F., Suomalainen, M., Stidwell, R. P., Boucke, K., Ebersold, M. W. & Helenius, A. ( 1997; ). The role of the nuclear pore complex in adenovirus DNA entry. EMBO J 16, 5998–6007.[CrossRef]
    [Google Scholar]
  13. Horn, P. J. & Peterson, C. L. ( 2002; ). Chromatin higher order folding: wrapping up transcription. Science 297, 1824–1827.[CrossRef]
    [Google Scholar]
  14. Korn, R. & Horwitz, M. S. ( 1986; ). Adenovirus DNA synthesis in vitro is inhibited by the virus-coded major core protein. Virology 150, 342–351.[CrossRef]
    [Google Scholar]
  15. Lunt, R., Vayda, M. E., Young, M. & Flint, S. J. ( 1988; ). Isolation and characterization of monoclonal antibodies against the adenovirus core proteins. Virology 164, 275–279.[CrossRef]
    [Google Scholar]
  16. Matthews, D. A. ( 2001; ). Adenovirus protein V induces redistribution of nucleolin and B23 from nucleolus to cytoplasm. J Virol 75, 1031–1038.[CrossRef]
    [Google Scholar]
  17. Matthews, D. A. & Russell, W. C. ( 1998; ). Adenovirus core protein V is delivered by the invading virus to the nucleus of the infected cell and later in infection is associated with the nucleoli. J Gen Virol 79, 1671–1675.
    [Google Scholar]
  18. Nakanishi, Y., Maeda, K., Ohtsuki, M., Hosokawa, K. & Natori, S. ( 1986; ). In vitro transcription of a chromatin-like complex of major core protein VII and DNA of adenovirus serotype 2. Biochem Biophys Res Commun 136, 86–93.[CrossRef]
    [Google Scholar]
  19. Russell, W. C. & Precious, B. ( 1982; ). Nucleic acid binding properties of adenovirus structural polypeptides. J Gen Virol 63, 69–79.[CrossRef]
    [Google Scholar]
  20. Sato, K. & Hosokawa, K. ( 1984; ). Analysis of the interaction between the DNA and major core protein in adenovirus chromatin by circular dichroism and ultraviolet light induced cross-linking. J Biochem 95, 1031–1039.
    [Google Scholar]
  21. Strahl, B. D. & Allis, C. D. ( 2000; ). The language of covalent histone modifications. Nature 403, 41–45.[CrossRef]
    [Google Scholar]
  22. Sung, M. T., Cao, T. M., Coleman, R. T. & Budelier, K. A. ( 1983; ). Gene and protein sequences of adenovirus protein VII, a hybrid basic chromosomal protein. Proc Natl Acad Sci U S A 80, 2902–2906.[CrossRef]
    [Google Scholar]
  23. Tarassishin, L., Szawlowski, P., McLay, J., Kidd, A. H. & Russell, W. C. ( 1999; ). Adenovirus core protein VII displays a linear epitope conserved in a range of human adenoviruses. J Gen Virol 80, 47–50.
    [Google Scholar]
  24. Urbani, L., Sherwood, S. W. & Schimke, R. T. ( 1995; ). Dissociation of nuclear and cytoplasmic cell cycle progression by drugs employed in cell synchronization. Exp Cell Res 219, 159–168.[CrossRef]
    [Google Scholar]
  25. Vayda, M. E. & Flint, S. J. ( 1987; ). Isolation and characterization of adenovirus core nucleoprotein subunits. J Virol 61, 3335–3339.
    [Google Scholar]
  26. Vayda, M. E., Rogers, A. E. & Flint, S. J. ( 1983; ). The structure of nucleoprotein cores released from adenovirions. Nucleic Acids Res 11, 441–460.[CrossRef]
    [Google Scholar]
  27. Walton, T. H., Moen, P. T., Jr, Fox, E. & Bodnar, J. W. ( 1989; ). Interactions of minute virus of mice and adenovirus with host nucleoli. J Virol 63, 3651–3660.
    [Google Scholar]
  28. Webster, A., Russell, S., Talbot, P., Russell, W. C. & Kemp, G. D. ( 1989; ). Characterization of the adenovirus proteinase: substrate specificity. J Gen Virol 70, 3225–3234.[CrossRef]
    [Google Scholar]
  29. Wienhues, U., Hosokawa, K., Hoveler, A., Siegmann, B. & Doerfler, W. ( 1987; ). A novel method for transfection and expression of reconstituted DNA–protein complexes in eukaryotic cells. DNA 6, 81–89.[CrossRef]
    [Google Scholar]
  30. Zhang, C. L., Nagaraja, K. V., Sivanandan, V. & Newman, J. A. ( 1991; ). Identification and characterization of viral polypeptides from type-II avian adenoviruses. Am J Vet Res 52, 1137–1141.
    [Google Scholar]
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