RT Journal Article SR Electronic(1) A1 de Boisséson, Claire A1 Béven, Véronique A1 Bigarré, Laurent A1 Thiéry, Richard A1 Rose, Nicolas A1 Eveno, Eric A1 Madec, François A1 Jestin, AndréYR 2004 T1 Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs JF Journal of General Virology, VO 85 IS 2 SP 293 OP 304 DO https://doi.org/10.1099/vir.0.19536-0 PB Microbiology Society, SN 1465-2099, AB Porcine circovirus type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied in pigs originating from PMWS-affected (+) and non-affected (−) herds from Brittany. PCV2 was identified by PCR in 100 % of animals from PMWS(+) herds and in 76 % from PMWS(−) herds, while PCV1 was not detected. The complete sequences of 38 PCV2 isolates were determined and 23 new variants were identified, displaying between 94·6 and 99·9 % nucleotide identity with one another. Although highly related to all the PCV2 sequences available in databases, the isolates from France gathered in a distinct subcluster. Compared with the 13 PCV2 from PMWS(+) farms, the 10 PMWS(−) sequences exhibited a slightly higher variability. No viral molecular marker specific to a pathogenic state could be identified, even by including other PCV2 variants isolated from PMWS-suffering animals from other countries. We concluded that the PMWS outbreaks in Brittany are most likely not due to the emergence of a new genotype of circovirus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19536-0