1887

Abstract

type 2 (PCV2) is recognized as a primary cause in post-weaning multisystemic wasting syndrome (PMWS). In this study, both PCV1 and PCV2 types were studied in pigs originating from PMWS-affected (+) and non-affected (−) herds from Brittany. PCV2 was identified by PCR in 100 % of animals from PMWS(+) herds and in 76 % from PMWS(−) herds, while PCV1 was not detected. The complete sequences of 38 PCV2 isolates were determined and 23 new variants were identified, displaying between 94·6 and 99·9 % nucleotide identity with one another. Although highly related to all the PCV2 sequences available in databases, the isolates from France gathered in a distinct subcluster. Compared with the 13 PCV2 from PMWS(+) farms, the 10 PMWS(−) sequences exhibited a slightly higher variability. No viral molecular marker specific to a pathogenic state could be identified, even by including other PCV2 variants isolated from PMWS-suffering animals from other countries. We concluded that the PMWS outbreaks in Brittany are most likely not due to the emergence of a new genotype of circovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19536-0
2004-02-01
2019-11-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/2/vir850293.html?itemId=/content/journal/jgv/10.1099/vir.0.19536-0&mimeType=html&fmt=ahah

References

  1. Allan, G. M. & Ellis, J. A. ( 2000; ). Porcine circoviruses: a review. J Vet Diagn Invest 12, 3–14.[CrossRef]
    [Google Scholar]
  2. Allan, G. M., Meehan, B., Todd, D. & 8 other authors ( 1998; ). Novel porcine circoviruses from pigs with wasting disease syndromes. Vet Rec 142, 467–468.
    [Google Scholar]
  3. Allan, G. M., McNeilly, F., Meehan, B. M. & 9 other authors ( 1999a; ). Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 66, 115–123.[CrossRef]
    [Google Scholar]
  4. Allan, G. M., Kennedy, S., McNeilly, F., Foster, J. C., Ellis, J. A., Krakowka, S. J., Meehan, B. M. & Adair, B. M. ( 1999b; ). Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121, 1–11.[CrossRef]
    [Google Scholar]
  5. Allan, G., McNeilly, F., Meehan, B. & 7 other authors ( 2002; ). Reproduction of PMWS with a 1993 Swedish isolate of PCV-2. Vet Rec 150, 255–256.
    [Google Scholar]
  6. Blanchard, P., Mahé. D., Cariolet, R. & 8 other authors ( 2003; ). An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome. Vet Microbiol 94, 183–194.[CrossRef]
    [Google Scholar]
  7. Calsamiglia, M., Segales, J., Quintana, J., Rosell, C. & Domingo, M. ( 2002; ). Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. J Clin Microbiol 40, 1848–1850.[CrossRef]
    [Google Scholar]
  8. Choi, C., Chae, C. & Clark, E. G. ( 2000; ). Porcine postweaning multisystemic wasting syndrome in Korean pig: detection of porcine circovirus 2 infection by immunohistochemistry and polymerase chain reaction. J Vet Diagn Invest 12, 151–153.[CrossRef]
    [Google Scholar]
  9. Choi, J., Stevenson, G. W., Kiupel, M., Harrach, B., Anothayanontha, L., Kanitz, C. L. & Mittal, S. K. ( 2002; ). Sequence analysis of old and new strains of porcine circovirus associated with congenital tremors in pigs and their comparison with strains involved with postweaning multisystemic wasting syndrome. Can J Vet Res 66, 217–224.
    [Google Scholar]
  10. Clark, E. G. ( 1997; ). Post-weaning multisystemic wasting syndrome. In Annual Meeting, pp. 499–501. Edited by the American Association of Swine Practitioners.
  11. Ellis, J. A., Bratanich, A., Clark, E. G. & 10 other authors ( 2000; ). Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12, 21–27.[CrossRef]
    [Google Scholar]
  12. Fenaux, M., Halbur, P. G., Gill, M., Toth, T. E. & Meng, X. J. ( 2000; ). Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR–restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38, 2494–2503.
    [Google Scholar]
  13. Fenaux, M., Halbur, P. G., Haqshenas, G., Royer, R., Thomas, P., Nawagitgul, P., Gill, M., Toth, T. E. & Meng, X. J. ( 2002; ). Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76, 541–551.[CrossRef]
    [Google Scholar]
  14. Giuffra, E., Kijas, J. M., Amarger, V., Carlborg, O., Jeon, J. T. & Andersson, L. ( 2000; ). The origin of the domestic pig: independent domestication and subsequent introgression. Genetics 154, 1785–1791.
    [Google Scholar]
  15. Hamel, A. L., Lin, L. L. & Nayar, G. P. S. ( 1998; ). Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72, 5262–5267.
    [Google Scholar]
  16. Harding, J. C. ( 1997; ). Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. In Annual Meeting, p. 503. Edited by the American Association of Swine Practitioners.
  17. Khudyakov, Y. E., Cong, M. E., Nichols, B. & 11 other authors ( 2000; ). Sequence heterogeneity of TT virus and closely related viruses. J Virol 74, 2990–3000.[CrossRef]
    [Google Scholar]
  18. Kiss, I., Kecskemeti, S., Tuboly, T., Bajmocy, E. & Tanyi, J. ( 2000; ). New pig disease in Hungary: post-weaning multisystemic wasting syndrome caused by circovirus. Acta Vet Hung 48, 469–475.
    [Google Scholar]
  19. Kiupel, M., Stevenson, G. W., Mittal, S. K., Clark, E. G. & Haines, D. M. ( 1998; ). Circovirus-like viral associated disease in weaned pigs in Indiana. Vet Pathol 35, 303–307.[CrossRef]
    [Google Scholar]
  20. Labarque, G. G., Nauwynck, H. J., Mesu, A. P. & Pensaert, M. B. ( 2000; ). Seroprevalence of porcine circovirus types 1 and 2 in the Belgian pig population. Vet Q 22, 234–236.[CrossRef]
    [Google Scholar]
  21. Larochelle, R., Antaya, M., Morin, M. & Magar, R. ( 1999; ). Typing of porcine circovirus in clinical specimens by multiplex PCR. J Virol Methods 80, 69–75.[CrossRef]
    [Google Scholar]
  22. Larochelle, R., Magar, R. & D’Allaire, S. ( 2002; ). Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) strains from cases presenting various clinical conditions. Virus Res 90, 101–112.[CrossRef]
    [Google Scholar]
  23. LeCann, P., Albina, E., Madec, F., Cariolet, R. & Jestin, A. ( 1997; ). Piglet wasting disease. Vet Rec 141, 660.
    [Google Scholar]
  24. Madec, F., Eveno, E., Morvan, P. & 9 other authors ( 2000; ). Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: clinical observations from follow-up studies on affected farms. Livest Prod Sci 63, 223–233.[CrossRef]
    [Google Scholar]
  25. Magar, R., Larochelle, R., Thibault, S. & Lamontagne, L. ( 2000; ). Experimental transmission of porcine circovirus type 2 (PCV2) in weaned pigs: a sequential study. J Comp Pathol 123, 258–269.[CrossRef]
    [Google Scholar]
  26. Maggi, F., Pistello, M., Vatteroni, M. & 8 other authors ( 2001; ). Dynamics of persistent TT virus infection, as determined in patients treated with alpha interferon for concomitant hepatitis C virus infection. J Virol 75, 11999–12004.[CrossRef]
    [Google Scholar]
  27. Mahé, D., Blanchard, P., Truong, C., Arnauld, C., Le Cann, P., Cariolet, R., Madec, F., Albina, E. & Jestin, A. ( 2000; ). Differential recognition of ORF2 protein from type 1 and type 2 porcine circovirus and identification of immunorelevant epitopes. J Gen Virol 81, 1815–1824.
    [Google Scholar]
  28. Mankertz, A., Domingo, M., Folch, J. M., LeCann, P., Jestin, A., Segales, J., Chmielewicz, B., Plana-Duran, J. & Soike, D. ( 2000; ). Characterisation of PCV-2 isolates from Spain, Germany and France. Virus Res 66, 65–77.[CrossRef]
    [Google Scholar]
  29. Onuki, A., Abe, K., Togashi, K., Kawashima, K., Taneichi, A. & Tsunemitsu, H. ( 1999; ). Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. J Vet Med Sci 61, 1119–1123.[CrossRef]
    [Google Scholar]
  30. Pogranichniy, R. M., Yoon, K. J., Harms, P. A., Sorden, S. D. & Daniels, M. ( 2002; ). Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome. J Vet Diagn Invest 14, 449–456.[CrossRef]
    [Google Scholar]
  31. Rodriguez-Arrioja, G., Segales, J., Calsamiglia, M., Resendes, A., Balasch, M., Plana-Duran, J., Casal, J. & Domingo, M. ( 2002; ). Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome. Am J Vet Res 63, 354–357.[CrossRef]
    [Google Scholar]
  32. Rose, N., Larour, G., Le Diguerher, G. & 7 other authors ( 2003; ). Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 French farrow-to-finish herds. Prev Vet Med 61, 209–225.[CrossRef]
    [Google Scholar]
  33. Rovira, A., Balasch, M., Segales, J., Garcia, L., Plana-Duran, J., Rosell, C., Ellerbrok, H., Mankertz, A. & Domingo, M. ( 2002; ). Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2. J Virol 76, 3232–3239.[CrossRef]
    [Google Scholar]
  34. Saoulidis, K., Kyriakis, S. C., Kennedy, S., Lekkas, S., Miliotis, Ch. C., Allan, G., Balkamos, G. C. & Papoutsis, P. A. ( 2002; ). First report of post-weaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome in pigs in Greece. J Vet Med Ser B Infect Dis Vet Public Health 49, 202–205.[CrossRef]
    [Google Scholar]
  35. Segales, J. & Domingo, M. ( 2002; ). Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. Vet Q 24, 109–124.[CrossRef]
    [Google Scholar]
  36. Segales, J., Sitjar, M., Domingo, M. & 7 other authors ( 1997; ). First report of post-weaning multisystemic wasting syndrome in pigs in Spain. Vet Rec 141, 600–601.
    [Google Scholar]
  37. Simmonds, P. ( 2002; ). TT virus infection: a novel virus–host relationship. J Med Microbiol 51, 455–458.
    [Google Scholar]
  38. Steinfeldt, T., Finsterbusch, T. & Mankertz, A. ( 2001; ). Rep and Rep′ protein of porcine circovirus type 1 bind to the origin of replication in vitro. Virology 291, 152–160.[CrossRef]
    [Google Scholar]
  39. Tischer, I., Rasch, R. & Tochtermann, G. ( 1974; ). Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentbl Bakteriol 226, 153–167.
    [Google Scholar]
  40. Todd, D., Scott, A. N., Ball, N. W., Borghmans, B. J. & Adair, B. M. ( 2002; ). Molecular basis of the attenuation exhibited by molecularly cloned highly passaged chicken anemia virus isolates. J Virol 76, 8472–8474.[CrossRef]
    [Google Scholar]
  41. Truyen, U., Gruenberg, A., Chang, S. F., Obermaier, B., Veijalainen, P. & Parrish, C. R. ( 1995; ). Evolution of the feline-subgroup parvoviruses and the control of canine host range in vivo. J Virol 69, 4702–4710.
    [Google Scholar]
  42. Wellenberg, G. J., Pesch, S., Berndsen, F. W. & 8 other authors ( 2000; ). Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in The Netherlands. Vet Q 22, 167–172.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19536-0
Loading
/content/journal/jgv/10.1099/vir.0.19536-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error