RT Journal Article SR Electronic(1) A1 Iwatsuki-Horimoto, Kiyoko A1 Kanazawa, Rie A1 Sugii, Shunji A1 Kawaoka, Yoshihiro A1 Horimoto, TaisukeYR 2004 T1 The index influenza A virus subtype H5N1 isolated from a human in 1997 differs in its receptor-binding properties from a virulent avian influenza virus JF Journal of General Virology, VO 85 IS 4 SP 1001 OP 1005 DO https://doi.org/10.1099/vir.0.19519-0 PB Microbiology Society, SN 1465-2099, AB To gain insight into the events that occur when avian influenza viruses are transmitted to humans, the receptor-binding properties of the index H5N1 influenza virus isolated from a human in 1997 and the A/turkey/Ontario/7732/66 (H5N9) virus were compared, by using a haemadsorption assay. Cells expressing the haemagglutinin (HA) of the human isolate were adsorbed by both chicken red blood cells (RBCs) and human RBCs; those expressing the avian virus HA were only adsorbed by chicken RBCs. These results indicate that human and avian influenza virus H5 HAs differ in their recognition of sialyloligosaccharides on the RBCs of different animal species. Mutational analyses indicated that differences in both the oligosaccharide chains and in the amino acid sequences around the HA receptor-binding site were responsible for this difference in receptor binding. These data further support the concept that alteration in receptor recognition is important for replication of avian viruses in humans., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19519-0