1887

Abstract

To gain insight into the events that occur when avian influenza viruses are transmitted to humans, the receptor-binding properties of the index H5N1 influenza virus isolated from a human in 1997 and the A/turkey/Ontario/7732/66 (H5N9) virus were compared, by using a haemadsorption assay. Cells expressing the haemagglutinin (HA) of the human isolate were adsorbed by both chicken red blood cells (RBCs) and human RBCs; those expressing the avian virus HA were only adsorbed by chicken RBCs. These results indicate that human and avian influenza virus H5 HAs differ in their recognition of sialyloligosaccharides on the RBCs of different animal species. Mutational analyses indicated that differences in both the oligosaccharide chains and in the amino acid sequences around the HA receptor-binding site were responsible for this difference in receptor binding. These data further support the concept that alteration in receptor recognition is important for replication of avian viruses in humans.

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2004-04-01
2019-11-14
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References

  1. Cheung, C. Y., Poon, L. L. M., Lau, A. S., Luk, W., Lau, Y. L., Shortridge, K. F., Gordon, S., Guan, Y. & Peiris, J. S. M. ( 2002; ). Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet 360, 1831–1837.[CrossRef]
    [Google Scholar]
  2. Claas, E. C., Osterhaus, A. D. M. E., van Beek, R., De Jong, J. C., Rimmelzwaan, G. F., Senne, D. A., Krauss, S., Shortridge, K. F. & Webster, R. G. ( 1998; ). Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet 351, 472–477.[CrossRef]
    [Google Scholar]
  3. Connor, R. J., Kawaoka, Y., Webster, R. G. & Paulson, J. C. ( 1994; ). Receptor specificity in human, avian, and equine H2 and H3 influenza virus isolates. Virology 205, 17–23.[CrossRef]
    [Google Scholar]
  4. Couceiro, J. N. S. S., Paulson, J. C. & Baum, L. G. ( 1993; ). Influenza virus strains selectively recognize sialyloligosaccharides on human respiratory epithelium: the role of the host cell in selection of hemagglutinin receptor specificity. Virus Res 29, 155–165.[CrossRef]
    [Google Scholar]
  5. Garcia, M., Crawford, J. M., Latimer, J. W., Rivera-Cruz, E. & Perdue, M. L. ( 1996; ). Heterogeneity in the haemagglutinin gene and emergence of the highly pathogenic phenotype among recent H5N2 avian influenza viruses from Mexico. J Gen Virol 77, 1493–1504.[CrossRef]
    [Google Scholar]
  6. Ha, Y., Stevens, D. J., Skehel, J. J. & Wiley, D. C. ( 2001; ). X-ray structures of H5 avian and H9 swine influenza virus hemagglutinins bound to avian and human receptor analogs. Proc Natl Acad Sci U S A 98, 11181–11186.[CrossRef]
    [Google Scholar]
  7. Ha, Y., Stevens, D. J., Skehel, J. J. & Wiley, D. C. ( 2002; ). H5 avian and H9 swine influenza virus haemagglutinin structures: possible origin of influenza subtypes. EMBO J 21, 865–875.[CrossRef]
    [Google Scholar]
  8. Hatta, M., Gao, P., Halfmann, P. & Kawaoka, Y. ( 2001; ). Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293, 1840–1842.[CrossRef]
    [Google Scholar]
  9. Horimoto, T. & Kawaoka, Y. ( 1994; ). Reverse genetics provides a direct evidence for a correlation of hemagglutinin cleavability and virulence of an avian influenza A virus. J Virol 68, 3120–3128.
    [Google Scholar]
  10. Horimoto, T., Fukuda, N., Iwatsuki-Horimoto, K. & 7 other authors ( 2004; ). Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003. J Vet Med Sci (in press).
    [Google Scholar]
  11. Ito, T., Suzuki, Y., Mitnaul, L., Vines, A., Kida, H. & Kawaoka, Y. ( 1997; ). Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species. Virology 227, 493–499.[CrossRef]
    [Google Scholar]
  12. Ito, T., Couseiro, J. N. S. S., Kelm, S. & 8 other authors ( 1998; ). Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J Virol 72, 7367–7373.
    [Google Scholar]
  13. Kobasa, D., Rodgeres, M. E., Wells, K. & Kawaoka, Y. ( 1997; ). Neuraminidase hemadsorption activity, conserved in avian influenza A viruses, does not influence viral replication in ducks. J Virol 71, 6706–6713.
    [Google Scholar]
  14. Matrosovich, M., Zhou, N., Kawaoka, Y. & Webster, R. G. ( 1999; ). The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties. J Virol 73, 1146–1155.
    [Google Scholar]
  15. Matrosovich, M., Tuzikov, A., Bovin, N., Gambaryan, A., Klimov, A., Castrucci, M. R., Donatelli, I. & Kawaoka, Y. ( 2000; ). Early alterations of the receptor-binding properties of H1, H2, and H3 avian influenza virus hemagglutinins after their introduction into mammals. J Virol 74, 8502–8512.[CrossRef]
    [Google Scholar]
  16. Medeiros, R., Escriou, N., Naffakh, N., Manuguerra, J.-C. & van der Werf, S. ( 2001; ). Hemagglutinin residues of recent human A (H3N2) influenza viruses that contribute to the inability to agglutinate chicken erythrocytes. Virology 289, 74–85.[CrossRef]
    [Google Scholar]
  17. Niwa, H., Yamamura, K. & Miyazaki, J. ( 1991; ). Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–199.[CrossRef]
    [Google Scholar]
  18. Nobusawa, E., Ishihara, H., Morishita, T., Sato, K. & Nakajima, K. ( 2000; ). Change in receptor-binding specificity of recent human influenza A viruses (H3N2): a single amino acid change in hemagglutinin altered its recognition of sialyloligosaccharides. Virology 278, 587–596.[CrossRef]
    [Google Scholar]
  19. Ohuchi, M., Feldmann, A., Ohuchi, R. & Klenk, H.-D. ( 1995; ). Neuraminidase is essential for fowl plague virus hemagglutinin to show hemagglutinating activity. Virology 212, 77–83.[CrossRef]
    [Google Scholar]
  20. Rogers, G. N. & D'Souza, B. L. ( 1989; ). Receptor binding properties of human and animal H1 influenza virus isolates. Virology 173, 317–322.[CrossRef]
    [Google Scholar]
  21. Seo, S. H., Hoffmann, E. & Webster, R. G. ( 2002; ). Lethal H5N1 influenza viruses escape host anti-viral cytokine responses. Nat Med 8, 950–954.[CrossRef]
    [Google Scholar]
  22. Shortridge, K. F. ( 1995; ). The next pandemic influenza virus? Lancet 346, 1210–1212.[CrossRef]
    [Google Scholar]
  23. Subbarao, K., Klimov, A., Katz, J. & 13 other authors ( 1998; ). Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279, 393–396.[CrossRef]
    [Google Scholar]
  24. Weis, W., Brown, J. H., Cusack, S., Paulson, J. C., Skehel, J. J. & Wiley, D. C. ( 1988; ). Structure of the influenza virus hemagglutinin complexed with its receptor, sialic acid. Nature 333, 426–431.[CrossRef]
    [Google Scholar]
  25. Wright, P. F. & Webster, R. G. ( 2001; ). Orthomyxoviruses. In Fields Virology, 4th edn, pp. 1533–1579. Edited by D. M. Knipe & P. M. Howley. Philadelphia: Lippincott -Williams & Wilkins.
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