Limited infection without evidence of replication by porcine endogenous retrovirus in guinea pigs Free

Abstract

Porcine endogenous retrovirus (PERV) may potentially be transmitted through porcine xenotransplantation products administered to humans. This study examined the feasibility of using guinea pigs as a model to characterize the infectivity of PERV. To enhance the susceptibility of guinea pigs to retroviral infection or genomic integration, moderate physiological or immunological changes were induced prior to exposing the animals to PERV. Quantitative PERV-specific PCR performed on all tested samples resulted in either undetectable or very low copy numbers of proviruses, even in animals possessing PERV-specific antibody responses. The low copy number of viral DNA detected suggests that PERV infected a limited number of cells. However, PERV DNA levels did not increase over time, suggesting no virus replication occurred. These results in the guinea pig are similar to previous observations of non-human primate cells that allow PERV infection but do not support PERV replication .

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2004-01-01
2024-03-28
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References

  1. Argaw T., Ritzhaupt A., Wilson C. A. 2002; Development of a real time quantitative PCR assay for detection of porcine endogenous retrovirus. J Virol Methods 106:97–106
    [Google Scholar]
  2. Xenotransplantation Subcommittee. 2000; Report of the Food and Drug Administration Subcommittee on Xenotransplantation: meeting of 13 January 2000, Center for Biologics and Evaluation and Research. Xenotransplantation 7:75–79
    [Google Scholar]
  3. Deng Y. M., Tuch B. E., Rawlinson W. D. 2000; Transmission of porcine endogenous retroviruses in severe combined immunodeficient mice xenotransplanted with fetal porcine pancreatic cells. Transplantation 70:1010–1016
    [Google Scholar]
  4. Forbes S. J., Themis M., Alison M. R., Selden C., Coutelle C., Hodgson H. J. 1998; Retroviral gene transfer to the liver in vivo during tri-iodothyronine induced hyperplasia. Gene Ther 5:552–555
    [Google Scholar]
  5. Heneine W., Tibell A., Switzer W. M. 7 other authors 1998; No evidence of infection with porcine endogenous retrovirus in recipients of porcine islet-cell xenografts. Lancet 352:695–699
    [Google Scholar]
  6. Jilbert A. R., Botten J. A., Miller D. S., Bertram E. M., Hall P. M., Kotlarski J., Burrell C. J. 1998; Characterization of age- and dose-related outcomes of duck hepatitis B virus infection. Virology 244:273–282
    [Google Scholar]
  7. Kitten O., Cosset F. L., Ferry N. 1997; Highly efficient retrovirus-mediated gene transfer into rat hepatocytes in vivo. Hum Gene Ther 8:1491–1494
    [Google Scholar]
  8. Kozlowski P. A., Black K. P., Shen L., Jackson S. 1995; High prevalence of serum IgA HIV-1 infection-enhancing antibodies in HIV-infected persons. Masking by IgG. J Immunol 154:6163–6173
    [Google Scholar]
  9. Lai L., Kolber-Simonds D., Park K. W. 11 other authors 2002; Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089–1092
    [Google Scholar]
  10. Lidbury B. A., Mahalingam S. 2000; Specific ablation of antiviral gene expression in macrophages by antibody-dependent enhancement of Ross River virus infection. J Virol 74:8376–8381
    [Google Scholar]
  11. Loss M., Arends H., Winkler M. 7 other authors 2001; Analysis of potential porcine endogenous retrovirus (PERV) transmission in a whole-organ xenotransplantation model without interfering microchimerism. Transpl Int 14:31–37
    [Google Scholar]
  12. Martin U., Winkler M. E., Id M. 7 other authors 2000; Productive infection of primary human endothelial cells by pig endogenous retrovirus (PERV). Xenotransplantation 7:138–142
    [Google Scholar]
  13. McLachlin J. R., Mittereder N., Daucher M. B., Kadan M., Eglitis M. A. 1993; Factors affecting retroviral vector function and structural integrity. Virology 195:1–5
    [Google Scholar]
  14. Morens D. M. 1994; Antibody-dependent enhancement of infection and the pathogenesis of viral disease. Clin Infect Dis 19:500–512
    [Google Scholar]
  15. Morens D. M., Halstead S. B. 1990; Measurement of antibody-dependent infection enhancement of four dengue virus serotypes by monoclonal and polyclonal antibodies. J Gen Virol 71:2909–2914
    [Google Scholar]
  16. Olsen C. W. 1993; A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination. Vet Microbiol 36:1–37
    [Google Scholar]
  17. Paradis K., Langford G., Long Z. 7 other authors 1999; Search for cross-species transmission of porcine endogenous retrovirus in patients treated with living pig tissue. The XEN 111 Study Group. Science 285:1236–1241
    [Google Scholar]
  18. Patience C., Takeuchi Y., Weiss R. A. 1997; Infection of human cells by an endogenous retrovirus of pigs. Nat Med 3:282–286
    [Google Scholar]
  19. Patience C., Patton G. S., Takeuchi Y., Weiss R. A., McClure M. O., Rydberg L., Breimer M. E. 1998; No evidence of pig DNA or retroviral infection in patients with short-term extracorporeal connection to pig kidneys. Lancet 352:699–701
    [Google Scholar]
  20. Rettinger S. D., Kennedy S. C., Wu X., Saylors R. L., Hafenrichter D. G., Flye M. W., Ponder K. P. 1994; Liver-directed gene therapy: quantitative evaluation of promoter elements by using in vivo retroviral transduction. Proc Natl Acad Sci U S A 91:1460–1464
    [Google Scholar]
  21. Ritzhaupt A., Van Der Laan L. J., Salomon D. R., Wilson C. A. 2002; Porcine endogenous retrovirus infects but does not replicate in nonhuman primate primary cells and cell lines. J Virol 76:11312–11320
    [Google Scholar]
  22. Schumacher J. M., Ellias S. A., Palmer E. P. 12 other authors 2000; Transplantation of embryonic porcine mesencephalic tissue in patients with PD. Neurology 54:1042–1050
    [Google Scholar]
  23. Specke V., Tacke S. J., Boller K., Schwendemann J., Denner J. 2001; Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal models. J Gen Virol 82:837–844
    [Google Scholar]
  24. Switzer W. M., Michler R. E., Shanmugam V. 13 other authors 2001; Lack of cross-species transmission of porcine endogenous retrovirus infection to nonhuman primate recipients of porcine cells, tissues, or organs. Transplantation 71:959–965
    [Google Scholar]
  25. Tacke S. J., Bodusch K., Berg A., Denner J. 2001; Sensitive and specific immunological detection methods for porcine endogenous retroviruses applicable to experimental and clinical xenotransplantation. Xenotransplantation 8:125–135
    [Google Scholar]
  26. Takeuchi Y., Patience C., Magre S., Weiss R. A., Banerjee P. T., Le Tissier P., Stoye J. P. 1998; Host range and interference studies of three classes of pig endogenous retrovirus. J Virol 72:9986–9991
    [Google Scholar]
  27. Todaro G. J., Benveniste R. E., Lieber M. M., Sherr C. J. 1974; Characterization of a type C virus released from the porcine cell line PK(15). Virology 58:65–74
    [Google Scholar]
  28. Tucker A., Belcher C., Moloo B., Bell J., Mazzulli T., Humar A., Hughes A., McArdle P., Talbot A. 2002; The production of transgenic pigs for potential use in clinical xenotransplantation: baseline clinical pathology and organ size studies. Xenotransplantation 9:203–208
    [Google Scholar]
  29. van der Laan L. J., Lockey C., Griffeth B. C. 8 other authors 2000; Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice. Nature 407:90–94
    [Google Scholar]
  30. Werlich T., Stiller K. J., Machnik G. 1999; Experimental studies on the stem cell concept of liver regeneration. II. Exp Toxicol Pathol 51:93–98
    [Google Scholar]
  31. Wilson C. A., Eiden M. V. 1991; Viral and cellular factors governing hamster cell infection by murine and gibbon ape leukemia viruses. J Virol 65:5975–5982
    [Google Scholar]
  32. Wilson C. A., Wong S., Muller J., Davidson C. E., Rose T. M., Burd P. 1998; Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells. J Virol 72:3082–3087
    [Google Scholar]
  33. Yin L., Lynch D., Sell S. 1999; Participation of different cell types in the restitutive response of the rat liver to periportal injury induced by allyl alcohol. J Hepatol 31:497–507
    [Google Scholar]
  34. Zinkernagel R. M. 1996; Immunology taught by viruses. Science 271:173–178
    [Google Scholar]
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