1887

Abstract

Molecular epidemiological studies of Kaposi's sarcoma-associated herpesvirus (KSHV) have concentrated on characterization of viral strains in tumour biopsy samples from Kaposi's sarcoma (KS) patients, mostly obtained in the United States and Europe. Tumour biopsies are a convenient source of viral DNA, as they have a high viral load compared to peripheral blood. However, sequences obtained from biopsies may not be representative of viral strains in asymptomatic subjects and information on ethnicity is often not available. Here, a population-based approach has been used to study the molecular and seroepidemiology of KSHV in isolated populations in Ecuador and Botswana. Amerindians in Ecuador had a variable prevalence of KSHV and all strains characterized were of subtype E, based on K1 sequencing. All Amerindian strains had predominant (P)-type K15 alleles and had sequences in both T0.7 and ORF 75 that appeared to be characteristic of these strains. The prevalence of KSHV in two ethnic groups in Botswana was extremely high. K1 sequences from both Bantu and San subjects were mostly of subtypes B and A5, which are typical of African KSHV strains, but the sequence from one San subject did not cluster with any known subtype. Considerable heterogeneity was seen in the T0.7 and ORF 75 genes in the San subjects and one had a minor (M)-type K15 allele. The heterogeneity of the KSHV strains found in these subjects from Botswana contrasts with the homogeneity of KSHV strains in Amerindians, reflecting differences in the evolutionary history of these populations.

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2004-01-01
2024-03-28
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References

  1. Alagiozoglou L., Sitas F., Morris L. 2000; Phylogenetic analysis of human herpesvirus-8 in South Africa and identification of a novel subgroup. J Gen Virol 81:2029–2038
    [Google Scholar]
  2. Benefice E., Barral H., Romo-Nunez Z. 1989; Ecology of health and nutrition in Ecuadorian Amazonia (province of Napo). I. The Siona-Secoya Indians of the Aguarico River. Bull Soc Pathol Exot Filiales 82:531–543 (in French
    [Google Scholar]
  3. Biggar R. J., Whitby D., Marshall V., Linhares A. C., Black F. 2000; Human herpesvirus 8 in Brazilian Amerindians: a hyperendemic population with a new subtype. J Infect Dis 181:1562–1568
    [Google Scholar]
  4. Cesarman E., Chang Y., Moore P. S., Said J. W., Knowles D. M. 1995; Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas. N Engl J Med 332:1186–1191
    [Google Scholar]
  5. Chang Y., Cesarman E., Pessin M. S., Lee F., Culpepper J., Knowles D. M., Moore P. S. 1994; Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science 266:1865–1869
    [Google Scholar]
  6. Cook P. M., Whitby D., Calabro M. L. 7 other authors 1999; Variability and evolution of Kaposi's sarcoma-associated herpesvirus in Europe and Africa. International Collaborative Group. . AIDS 13:1165–1176
    [Google Scholar]
  7. de Sanjose S., Marshall V., Sola J., Palacio V., Almirall R., Goedert J. J., Bosch F. X., Whitby D. 2002; Prevalence of Kaposi's sarcoma-associated herpesvirus infection in sex workers and women from the general population in Spain. Int J Cancer 98:155–158
    [Google Scholar]
  8. Ebbesen P., Osei W. D., Manganu E. T. 1989; San people (bushmen) from the central Kalahari desert found free of antibodies to HIV-1 and HIV-2. J Acquir Immune Defic Syndr 2:415–416
    [Google Scholar]
  9. Engels E. A., Sinclair M. D., Biggar R. J., Whitby D., Ebbesen P., Goedert J. J., Gastwirth J. L. 2000a; Latent class analysis of human herpesvirus 8 assay performance and infection prevalence in sub-saharan Africa and Malta. Int J Cancer 88:1003–1008
    [Google Scholar]
  10. Engels E. A., Whitby D., Goebel P. B., Stossel A., Waters D., Pintus A., Contu L., Biggar R. J., Goedert J. J. 2000b; Identifying human herpesvirus 8 infection: performance characteristics of serologic assays. J Acquir Immune Defic Syndr 23:346–354
    [Google Scholar]
  11. Franceschi S., Geddes M. 1995; Epidemiology of classic Kaposi's sarcoma, with special reference to mediterranean population. Tumori 81:308–314
    [Google Scholar]
  12. Goedert J. J., Kedes D. H., Ganem D. 1997; Antibodies to human herpesvirus 8 in women and infants born in Haiti and the USA. Lancet 349:1368
    [Google Scholar]
  13. Hayward G. S. 1999; KSHV strains: the origins and global spread of the virus. Semin Cancer Biol 9:187–199
    [Google Scholar]
  14. Kakoola D. N., Sheldon J., Byabazaire N., Bowden R. J., Katongole-Mbidde E., Schulz T. F., Davison A. J. 2001; Recombination in human herpesvirus-8 strains from Uganda and evolution of the K15 gene. J Gen Virol 82:2393–2404
    [Google Scholar]
  15. Kaposi M. 1872; Idiopathisches multiples pigmentsarcom der haut. Arch Dermatol Syph 4:265–273
    [Google Scholar]
  16. Kron M. A., Ammunariz M., Pandey J., Guzman J. R. 2000; Hyperimmunoglobulinemia E in the absence of atopy and filarial infection: the Huaorani of Ecuador. Allergy Asthma Proc 21:335–341
    [Google Scholar]
  17. Lacoste V., Judde J.-G., Brière J. 11 other authors 2000; Molecular epidemiology of human herpesvirus 8 in Africa: both B and A5 K1 genotypes, as well as the M and P genotypes of K14.1/K15 loci, are frequent and widespread. Virology 278:60–74
    [Google Scholar]
  18. Meng Y.-X., Spira T. J., Bhat G. J. 9 other authors 1999; Individuals from North America, Australasia, and Africa are infected with four different genotypes of human herpesvirus 8. Virology 261:106–119
    [Google Scholar]
  19. Meng Y.-X., Lam L. L., Kite-Powell K., Stamey F. R., Pau C. P., Pellett P. E., Spira T. J. 2000; Human immunodeficiency virus-seropositive individual with persistent human herpesvirus 8 infection for >11 years without development of Kaposi's sarcoma. Clin Infect Dis 30:221–222
    [Google Scholar]
  20. Meng Y.-X., Sata T., Stamey F. R. 7 other authors 2001; Molecular characterization of strains of Human herpesvirus 8 from Japan. Argentina and Kuwait. J Gen Virol 82:499–506
    [Google Scholar]
  21. Moore P. S., Kingsley L. A., Holmberg S. D. 7 other authors 1996; Kaposi's sarcoma-associated herpesvirus infection prior to onset of Kaposi's sarcoma. AIDS 10:175–180
    [Google Scholar]
  22. Oettle A. G. 1962; Geographical and racial differences in the frequency of Kaposi's sarcoma as evidence of environmental or genetic causes. In Symposium on Kaposi's sarcoma Edited by Ackerman L. V., Murray J. F. Basel: Karger;
    [Google Scholar]
  23. Penn I. 1978; Development of cancer in transplantation patients. Adv Surg 12:155–191
    [Google Scholar]
  24. Poole L. J., Zong J.-C., Ciufo D. M., Alcendor D. J., Cannon J. S., Ambinder R., Orenstein J. M., Reitz M. S., Hayward G. S. 1999; Comparison of genetic variability at multiple loci across the genomes of the major subtypes of Kaposi's sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame K15 alleles at the right-hand end. J Virol 73:6646–6660
    [Google Scholar]
  25. Schulz T. F., Sheldon J., Greensill J. 2002; Kaposi's sarcoma associated herpesvirus (KSHV) or human herpesvirus 8 (HHV8). Virus Res 82:115–126
    [Google Scholar]
  26. Simpson G. R., Schulz T. F., Whitby D. 14 other authors 1996; Prevalence of Kaposi's sarcoma associated herpesvirus infection measured by antibodies to recombinant capsid protein and latent immunofluorescence antigen. Lancet 348:1133–1138
    [Google Scholar]
  27. Sitas F., Newton R. 2001; Kaposi's sarcoma in South Africa. J Natl Cancer Inst Monogr 28:1–4
    [Google Scholar]
  28. Smith N. A., Sabin C. A., Gopal R. 11 other authors 1999; Serologic evidence of human herpesvirus 8 transmission by homosexual but not heterosexual sex. J Infect Dis 180:600–606
    [Google Scholar]
  29. Soulier J., Grollet L., Oksenhendler E. 8 other authors 1995; Kaposi's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman's disease. Blood 86:1276–1280
    [Google Scholar]
  30. Wabinga H. R., Parkin D. M., Wabwire-Mangen F., Mugerwa J. W. 1993; Cancer in Kampala, Uganda, in 1989–91: changes in incidence in the era of AIDS. Int J Cancer 54:26–36
    [Google Scholar]
  31. Whitby D., Howard M. R., Tenant-Flowers M. 8 other authors 1995; Detection of Kaposi sarcoma associated herpesvirus in peripheral blood of HIV-infected individuals and progression to Kaposi's sarcoma. Lancet 346:799–802
    [Google Scholar]
  32. Yuan C. C., Miley W., Waters D. 2001; A quantification of human cells using an ERV-3 real time PCR assay. J Virol Methods 91:109–117
    [Google Scholar]
  33. Zong J.-C., Ciufo D. M., Alcendor D. J. 14 other authors 1999; High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi's sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. J Virol 73:4156–4170
    [Google Scholar]
  34. Zong J., Ciufo D. M., Viscidi R. 11 other authors 2002; Genotypic analysis at multiple loci across Kaposi's sarcoma herpesvirus (KSHV) DNA molecules: clustering patterns, novel variants and chimerism. J Clin Virol 23:119–148
    [Google Scholar]
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