1887

Abstract

Human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) Vif share limited homology and display species-specific activity, leading to speculation that Vif sequences could determine the block in HIV-1 replication in rhesus monkeys. To address this issue, we engineered a novel SIV recombinant in which HIV-1 replaced SIV in a SIVmac239 background. Insertion of HIV-1 into the SIV locus did not produce a replication-competent virus. Therefore, we inserted HIV-1 sequences into the SIV locus, which produced a recombinant that, in the absence of SIV sequences, replicated similarly to wild-type SIVmac239 in rhesus monkey PBMC. From these studies we conclude that the HIV-1 replication block in rhesus monkeys is almost certainly not Vif determined. These studies also suggest that SHIV/NVif or derivative sequences could be utilized for structure/function studies of HIV-1 Vif in experimentally infected rhesus monkeys.

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2003-12-01
2019-10-23
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