1887

Abstract

Human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) Vif share limited homology and display species-specific activity, leading to speculation that Vif sequences could determine the block in HIV-1 replication in rhesus monkeys. To address this issue, we engineered a novel SIV recombinant in which HIV-1 replaced SIV in a SIVmac239 background. Insertion of HIV-1 into the SIV locus did not produce a replication-competent virus. Therefore, we inserted HIV-1 sequences into the SIV locus, which produced a recombinant that, in the absence of SIV sequences, replicated similarly to wild-type SIVmac239 in rhesus monkey PBMC. From these studies we conclude that the HIV-1 replication block in rhesus monkeys is almost certainly not Vif determined. These studies also suggest that SHIV/NVif or derivative sequences could be utilized for structure/function studies of HIV-1 Vif in experimentally infected rhesus monkeys.

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2003-12-01
2024-04-24
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References

  1. Alexander L., Du Z., Rosenzweig M., Jung J. U., Desrosiers R. C. 1997; A role for natural simian immunodeficiency virus and human immunodeficiency virus type 1 nef alleles in lymphocyte activation. J Virol 71:6094–6099
    [Google Scholar]
  2. Alexander L., Du Z., Howe A. Y., Czajak S., Desrosiers R. C. 1999a; Induction of AIDS in rhesus monkeys by a recombinant simian immunodeficiency virus expressing nef of human immunodeficiency virus type 1. J Virol 73:5814–5825
    [Google Scholar]
  3. Alexander L., Veazey R. S., Czajak S., DeMaria M., Rosenzweig M., Lackner A. A., Desrosiers R. C., Sasseville V. G. 1999b; Recombinant simian immunodeficiency virus expressing green fluorescent protein identifies infected cells in rhesus monkeys. AIDS Res Hum Retrovir 15:11–21
    [Google Scholar]
  4. Alexander L., Weiskopf E., Greenough T. C. 7 other authors 2000; Unusual polymorphisms in human immunodeficiency virus type 1 associated with nonprogressive infection. J Virol 74:4361–4376
    [Google Scholar]
  5. Desrosiers R. C. 2001; Nonhuman lentiviruses. In Fields Virology . , 4th edn. pp  2095–2122 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
  6. Freed E. O., Martin M. A. 2001; HIVs and their replication. In Fields Virology , 4th edn. pp  1971–2042 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  7. Gibbs J. S., Regier D. A., Desrosiers R. C. 1994; Construction and in vitro properties of SIVmac mutants with deletions in “nonessential” genes. AIDS Res Hum Retrovir 10:333–342
    [Google Scholar]
  8. Karlsson G. B., Halloran M., Li J. 7 other authors 1997; Characterization of molecularly cloned simian–human immunodeficiency viruses causing rapid CD4+ lymphocyte depletion in rhesus monkeys. J Virol 71:4218–4225
    [Google Scholar]
  9. Kestler H. W. III, Ringler D. J., Mori K., Panicali D. L., Sehgal P. K., Daniel M. D., Desrosiers R. C. 1991; Importance of the nef gene for maintenance of high virus loads and for development of AIDS. Cell 65:651–662
    [Google Scholar]
  10. Kirchhoff F., Munch J., Carl S. 8 other authors 1999; The human immunodeficiency virus type 1 nef gene can to a large extent replace simian immunodeficiency virus nef in vivo. J Virol 73:8371–8383
    [Google Scholar]
  11. Kozak M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res 9:5233–5262
    [Google Scholar]
  12. Kozak M. 1987; An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res 15:8125–8148
    [Google Scholar]
  13. Munk C., Brandt S. M., Lucero G., Landau N. R. 2002; A dominant block to HIV-1 replication at reverse transcription in simian cells. Proc Natl Acad Sci U S A 99:13843–13848
    [Google Scholar]
  14. Naidu Y. M., Kestler H. W. III, Li Y., other authors. 1988; Characterization of infectious molecular clones of simian immunodeficiency virus (SIVmac) and human immunodeficiency virus type 2: persistent infection of rhesus monkeys with molecularly cloned SIVmac. J Virol 62:4691–4696
    [Google Scholar]
  15. Owens C. M., Yang P. C., Gottlinger H., Sodroski J. 2003; Human and simian immunodeficiency virus capsid proteins are major viral determinants of early, postentry replication blocks in simian cells. J Virol 77:726–731
    [Google Scholar]
  16. Regier D. A., Desrosiers R. C. 1990; The complete nucleotide sequence of a pathogenic molecular clone of simian immunodeficiency virus. AIDS Res Hum Retrovir 6:1221–1231
    [Google Scholar]
  17. Reimann K. A., Li J. T., Veazey R., Halloran M., Park I. W., Karlsson G. B., Sodroski J., Letvin N. L. 1996a; A chimeric simian/human immunodeficiency virus expressing a primary patient human immunodeficiency virus type 1 isolate env causes an AIDS-like disease after in vivo passage in rhesus monkeys. J Virol 70:6922–6928
    [Google Scholar]
  18. Reimann K. A., Li J. T., Voss G. 10 other authors 1996b; An env gene derived from a primary human immunodeficiency virus type 1 isolate confers high in vivo replicative capacity to a chimeric simian/human immunodeficiency virus in rhesus monkeys. J Virol 70:3198–3206
    [Google Scholar]
  19. Sheehy A. M., Gaddis N. C., Choi J. D., Malim M. H. 2002; Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 418:646–650
    [Google Scholar]
  20. Simon J. H., Southerling T. E., Peterson J. C., Meyer B. E., Malim M. H. 1995; Complementation of vif -defective human immunodeficiency virus type 1 by primate, but not nonprimate, lentivirus vif genes. J Virol 69:4166–4172
    [Google Scholar]
  21. Simon J. H., Miller D. L., Fouchier R. A., Soares M. A., Peden K. W., Malim M. H. 1998; The regulation of primate immunodeficiency virus infectivity by Vif is cell species restricted: a role for Vif in determining virus host range and cross-species transmission. EMBO J 17:1259–1267
    [Google Scholar]
  22. Soderberg K., Denekamp L., Nikiforow S., Sautter K., Desrosiers R. C., Alexander L. 2002; A nucleotide substitution in the tRNAlys dramatically increases replication of recombinant simian immunodeficiency virus containing a human immunodeficiency virus type I reverse transcriptase. J Virol 76:5803–5806
    [Google Scholar]
  23. Uberla K., Stahl-Hennig C., Bottiger D., Matz-Rensing K., Kaup F. J. 1995; Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors. Proc Natl Acad Sci U S A 92:8210–8214
    [Google Scholar]
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