1887

Abstract

Virions of the alphanodavirus (PaV) have =3 icosahedral symmetry and are assembled from multiple copies of a precursor protein that is cleaved into two mature capsid proteins after assembly. The crystal structure of PaV shows that the N-terminal ∼30 amino acid residues of the subunits surrounding the 5-fold axes interact extensively with icosahedrally ordered regions of the encapsidated positive-sense genomic RNAs. We found that wild-type PaV particles also contain a minor capsid protein that is truncated by 24 residues at its N terminus. Reverse genetic experiments showed that translation of this protein initiated at the second AUG of the capsid protein open reading frame. When either the longer or shorter version of the capsid protein was expressed independently of the other, it assembled into virus particles and underwent maturational cleavage. Virions that lacked the shorter capsid protein retained infectivity for cultured insect cells and larvae.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19419-0
2003-10-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/10/vir842847.html?itemId=/content/journal/jgv/10.1099/vir.0.19419-0&mimeType=html&fmt=ahah

References

  1. Ball, L. A. & Johnson, K. L. ( 1998; ). Nodaviruses of insects. In The Insect Viruses, pp. 225–267. Edited by L. A. Ball. New York: Plenum.
  2. Ball, L. A., Hendry, D. A., Johnson, J. E., Rueckert, R. R. & Scotti, P. D. ( 2000; ). Nodaviridae. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, pp. 747–755. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  3. Buchholz, U. J., Finke, S. & Conzelmann, K. K. ( 1999; ). Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73, 251–259.
    [Google Scholar]
  4. Cavener, D. R. & Ray, S. C. ( 1991; ). Eukaryotic start and stop translation sites. Nucleic Acids Res 19, 3185–3192.[CrossRef]
    [Google Scholar]
  5. Dong, X. F., Natarajan, P., Tihova, M., Johnson, J. E. & Schneemann, A. ( 1998; ). Particle polymorphism caused by deletion of a peptide molecular switch in a quasi-equivalent icosahedral virus. J Virol 72, 6024–6033.
    [Google Scholar]
  6. Gallagher, T. M. & Rueckert, R. R. ( 1988; ). Assembly-dependent maturation cleavage in provirions of a small icosahedral insect ribovirus. J Virol 62, 3399–3406.
    [Google Scholar]
  7. Hosur, M. V., Schmidt, T., Tucker, R. C., Johnson, J. E., Gallagher, T. M., Selling, B. H. & Rueckert, R. R. ( 1987; ). Structure of an insect virus at 3·0 Angstrom resolution. Proteins 2, 167–176.[CrossRef]
    [Google Scholar]
  8. Johnson, K. N. & Ball, L. A. ( 2001; ). Recovery of infectious Pariacoto virus from cDNA clones and identification of susceptible cell lines. J Virol 75, 12220–12227.[CrossRef]
    [Google Scholar]
  9. Johnson, K. N., Zeddam, J. & Ball, L. A. ( 2000; ). Characterization and construction of functional cDNA clones of Pariacoto virus, the first Alphanodavirus isolated outside Australasia. J Virol 74, 5123–5132.[CrossRef]
    [Google Scholar]
  10. Johnson, K. N., Johnson, K. L., Dasgupta, R., Gratsch, T. & Ball, L. A. ( 2001; ). Comparisons among the larger genome segments of six nodaviruses and their encoded RNA replicases. J Gen Virol 82, 1855–1866.
    [Google Scholar]
  11. Johnson, K. L., Price, B. D. & Ball, L. A. ( 2003; ). Recovery of infectivity from cDNA clones of Nodamura virus and identification of small nonstructural proteins. Virology 305, 436–451.[CrossRef]
    [Google Scholar]
  12. Kaesberg, P., Dasgupta, R., Sgro, J.-Y., Wery, J.-P., Selling, B. H., Hosur, M. V. & Johnson, J. E. ( 1990; ). Structural homology among four nodaviruses as deduced by sequencing and X-ray crystallography. J Mol Biol 214, 423–435.[CrossRef]
    [Google Scholar]
  13. Kariuki, C. W., McIntosh, A. H. & Goodman, C. L. ( 2000; ). In vitro host range studies with a new baculovirus isolate from the diamondback moth Plutella xylostella (L.) (Plutellidae: Lepidoptera). In Vitro Cell Dev Biol Anim 36, 271–276.[CrossRef]
    [Google Scholar]
  14. Krishna, N. K. & Schneemann, A. ( 1999; ). Formation of an RNA heterodimer upon heating of nodavirus particles. J Virol 73, 1699–1703.
    [Google Scholar]
  15. Krishna, N. K., Marshall, D. & Schneemann, A. ( 2003; ). Analysis of RNA packaging in wild-type and mosaic protein capsids of Flock House virus using recombinant baculovirus vectors. Virology 305, 10–24.[CrossRef]
    [Google Scholar]
  16. Marshall, D. & Schneemann, A. ( 2001; ). Specific packaging of nodaviral RNA2 requires the N-terminus of the capsid protein. Virology 285, 165–175.[CrossRef]
    [Google Scholar]
  17. Price, B. D., Roeder, M. & Ahlquist, P. ( 2000; ). DNA-directed expression of functional Flock House virus RNA1 derivatives in Saccharomyces cerevisiae, heterologous gene expression, and selective effects on subgenomic mRNA synthesis. J Virol 74, 11724–11733.[CrossRef]
    [Google Scholar]
  18. Sambrook, J. & Russell, D. W. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Schneemann, A., Zhong, W., Gallagher, T. M. & Rueckert, R. R. ( 1992; ). Maturation cleavage required for infectivity of a nodavirus. J Virol 66, 6728–6734.
    [Google Scholar]
  20. Schneemann, A., Reddy, V. & Johnson, J. E. ( 1998; ). The structure and function of nodavirus particles: a paradigm for understanding chemical biology. Adv Virus Res 50, 381–446.
    [Google Scholar]
  21. Tang, L., Johnson, K. N., Ball, L. A., Lin, T., Yeager, M. & Johnson, J. E. ( 2001; ). The structure of Pariacoto virus reveals a dodecahedral cage of duplex RNA. Nature Struct Biol 8, 77–83.[CrossRef]
    [Google Scholar]
  22. Wery, J.-P., Reddy, V. S., Hosur, M. V. & Johnson, J. E. ( 1994; ). The refined three-dimensional structure of an insect virus at 2·8 Å resolution. J Mol Biol 235, 565–586.[CrossRef]
    [Google Scholar]
  23. Zeddam, J. L., Rodriguez, J. L., Ravallec, M. & Lagnaoui, A. ( 1999; ). A noda-like virus isolated from the sweetpotato pest Spodoptera eridania (Cramer) (Lep; Noctuidae). J Invertebr Pathol 74, 267–274.[CrossRef]
    [Google Scholar]
  24. Zlotnick, A., Reddy, V. S., Dasgupta, R., Schneemann, A., Ray, W. J., Jr, Rueckert, R. R. & Johnson, J. E. ( 1994; ). Capsid assembly in a family of animal viruses primes an autoproteolytic maturation that depends on a single aspartic acid residue. J Biol Chem 269, 13680–13684.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19419-0
Loading
/content/journal/jgv/10.1099/vir.0.19419-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error