1887

Abstract

The complete sequence of the Pelargonium zonate spot virus (PZSV) genome was determined. It comprises 8477 nt, distributed in three positive-strand RNA species encoding four proteins. RNA-1 is 3383 nt long, with an ORF that encodes a polypeptide with a molecular mass of 108 419 Da (denoted protein 1a). This protein contains the conserved sequence motifs I–III of type I methyltransferases and the seven consensus motifs of the helicases of superfamily 1. RNA-2 is 2435 nt long and encodes a major polypeptide with a molecular mass of 78 944 Da (denoted protein 2a), which shows identity to the RNA-dependent RNA polymerases of positive-strand RNA viruses. RNA-3 is 2659 nt long and contains two major ORFs. The first ORF is located in the 5′ portion of the genome and sequence comparison of the putative translation product revealed similarities with the 30K superfamily of virus movement proteins. The second ORF is located in the 3′ half and encodes the viral coat protein, which is expressed via a subgenomic RNA, RNA-4. The transcription initiation site of RNA-4 maps to the intergenic region of RNA-3. The organization of the PZSV genome, including the primary structure of terminal non-coding regions, strongly suggests that this virus belongs to the family . The overall biological and genomic characteristics of PZSV indicate affinities in diverging directions with one or other of the virus species in this family, thus enabling it to be considered as a possible representative of a new genus within the family .

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2003-11-01
2021-01-23
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References

  1. Anon. 1994; Program Manual for the Wisconsin Package , version 8 Genetic Computer Group; Madison, WI, USA:
    [Google Scholar]
  2. Bol J. F.. 1999; Alfalfa mosaic virus and ilarviruses: involvement of coat protein in multiple steps of the replication cycle. J Gen Virol80:1089–1102
    [Google Scholar]
  3. Buck K. W.. 1996; Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res47:159–251
    [Google Scholar]
  4. Burgyan J., Russo M., Gallitelli D.. 1986; Translation of Cymbidium ringspot virus RNA in cowpea protoplasts and rabbit reticulocyte lysates. J Gen Virol67:1149–1160
    [Google Scholar]
  5. Calisher C. E., Carstens E. B., Christian P., Mahy B. W., Mayo M. A., Shope R. E.. 2000; Unassigned viruses. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses pp 995–1008 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B.. San Diego: Academic Press;
    [Google Scholar]
  6. DeBorde D. C., Naeve C. W., Herlocher M. L., Maassab H. F.. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Anal Biochem157:275–282
    [Google Scholar]
  7. Felsenstein J.. 1989; phylip: Phylogeny Inference Package, version 3.5. Cladistics5:164–166
    [Google Scholar]
  8. Fichot O., Girard M.. 1990; An improved method for sequencing of RNA templates. Nucleic Acids Res18:6162
    [Google Scholar]
  9. Finetti-Sialer M. M., Di Franco A., Papanice M. A., Gallitelli D.. 1997; Tomato necrotic yellows induced by a novel strain of alfalfa mosaic virus. J Plant Pathol79:115–120
    [Google Scholar]
  10. Finetti-Sialer M. M., Cillo F., Barbarossa L., Gallitelli D.. 1999; Differentiation of cucumber mosaic virus subgroups by RT-PCR RFLP. J Plant Pathol81:145–148
    [Google Scholar]
  11. Gallitelli D.. 1982; Properties of tomato isolate of Pelargonium zonate spot virus. Ann Appl Biol100:457–466
    [Google Scholar]
  12. Gallitelli D., Martelli G. P., Quacquarelli A.. 1983; Pelargonium zonate spot virus. Plant Viruses272:4
    [Google Scholar]
  13. Gallitelli D., Hull R., Koenig R.. 1985; Relationships among viruses in the Tombusvirus group: nucleic acid hybridization studies. J Gen Virol66:1523–1531
    [Google Scholar]
  14. Gebre-Selassie K., Delecolle B., Gognalons P., Dufour O., Gros C., Cotillon A. C., Parrella G., Marchoux G.. 2002; First report of an isolate of Pelargonium zonate spot virus in commercial glasshouse tomato crops in Southeastern France. Plant Dis86:1052
    [Google Scholar]
  15. Greber R. S., Teakle D. S., Mink G. I.. 1992; Thrips-facilitated transmission of prune dwarf and Prunus necrotic ringspot viruses from cherry pollen to cucumber. Plant Dis76:1039–1041
    [Google Scholar]
  16. Grieco F., Savino V., Martelli G. P.. 1996; Nucleotide sequence of the genome of a citrus isolate of olive latent virus 1. Arch Virol141:825–838
    [Google Scholar]
  17. Koonin E. V., Dolja V. V.. 1993; Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol28:375–430 erratum 28, 546
    [Google Scholar]
  18. Luis-Arteaga M., Cambra M.. 2000; First report of natural infection of greenhouse-grown tomato and weed species by Pelargonium zonate spot virus in Spain. Plant Dis84:807
    [Google Scholar]
  19. Lütcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A.. 1987; Selection of AUG initiation codons differs in plants and animals. EMBO J6:43–48
    [Google Scholar]
  20. Marck C.. 1988; ‘DNA Strider’: a ‘C’ programme for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res16:1829–1836
    [Google Scholar]
  21. Marsh L. E., Dreher T. W., Hall T. C.. 1988; Mutational analysis of the core and modulator sequences of the BMV RNA-3 subgenomic promoter. Nucleic Acids Res16:981–995
    [Google Scholar]
  22. Melcher U.. 2000; The ‘30K’ superfamily of viral movement proteins. J Gen Virol81:257–266
    [Google Scholar]
  23. Milner J. J., Jackson A. O.. 1979; Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polysomes of infected tobacco. Virology97:90–99
    [Google Scholar]
  24. Pogue G. P., Marsh L. E., Connell J. P., Hall T. C.. 1992; Requirement for ICR-like sequences in the replication of Brome mosaic virus genomic RNA. Virology188:742–753
    [Google Scholar]
  25. Quacquarelli A., Gallitelli D.. 1979; Three viruses of Pelargonium zonale in Apulia. Phytopathol Mediterr18:61–70 (in Italian
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Sdoodee R., Teakle D. S.. 1987; Transmission of tobacco streak virus by Thrips tabaci : a new method of plant virus transmission. Plant Path36:377–380
    [Google Scholar]
  28. Vovlas C., Gallitelli D., Di Franco A.. 1986; Epiphytotics of Pelargonium zonate spot virus in tomato in Apulia. Inf Fitopatol36:39–41 (in Italian
    [Google Scholar]
  29. Vovlas C., Gallitelli D., Conti M.. 1989; Preliminary evidence for an unusual mode of transmission in the ecology of Pelargonium zonate spot virus (PZSV. IVth International Plant Virus Epidemiology Workshop pp 302–305 (Montpellier, France: 3–8 September 1989
    [Google Scholar]
  30. Yoshinari S., Dreher T. W.. 2000; Internal and 3′ RNA initiation by Q β replicase directed by CCA boxes. Virology271:363–370
    [Google Scholar]
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