1887

Abstract

The nucleocapsid (N) protein of the pneumovirus respiratory syncytial virus (RSV) is a major structural protein which encapsidates the RNA genome and is essential for replication and transcription of the RSV genome. The N protein of the related virus pneumonia virus of mice (PVM) is functionally unable to replace the RSV N protein in a minigenome replication assay. Using chimeric proteins, in which the immediate C-terminal part of the RSV N protein was replaced with the equivalent region of the PVM N protein, it was shown that six amino acid residues near the C terminus of the N protein (between residues 352–369) are essential for its function in replication and for the ability of the N protein to bind to the viral phosphoprotein, P.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19370-0
2003-10-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/10/vir842679.html?itemId=/content/journal/jgv/10.1099/vir.0.19370-0&mimeType=html&fmt=ahah

References

  1. Barr J., Easton A. J. 1995; Characterisation of the interaction between the nucleoprotein and phosphoprotein of pneumonia virus of mice. Virus Res 39:221–235
    [Google Scholar]
  2. Barr J., Chambers P., Pringle C. R., Easton A. J. 1991; Sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. J Gen Virol 72:677–685
    [Google Scholar]
  3. Bhella D., Ralph A., Murphy L. B., Yeo R. P. 2002; Significant differences in nucleocapsid morphology within the Paramyxoviridae . J Gen Virol 83:1831–1839
    [Google Scholar]
  4. Collins P. L., Hill M. G., Cristina J., Grosfeld H. 1996; Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proc Natl Acad Sci U S A 93:81–85
    [Google Scholar]
  5. Curran J., Marq J. B., Kolakofsky D. 1995; An N-terminal domain of the Sendai paramyxovirus P protein acts as a chaperone for the NP protein during the nascent chain assembly step of genome replication. J Virol 69:849–855
    [Google Scholar]
  6. Fuerst T. R., Niles E. G., Studier F. W., Moss B. 1986; Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A 83:8122–8126
    [Google Scholar]
  7. Garcia J., Garcia-Barreno B., Martinez I., Melero J. A. 1993a; Mapping of monoclonal antibody epitopes of the human respiratory syncytial virus P protein. Virology 195:239–242
    [Google Scholar]
  8. Garcia J., Garcia-Barreno B., Vivo A., Melero J. A. 1993b; Cytoplasmic inclusions of respiratory syncytial virus-infected cells: formation of inclusion bodies in transfected cells that coexpress the nucleoprotein, the phosphoprotein, and the 22K protein. Virology 195:243–247
    [Google Scholar]
  9. Garcia-Barreno B., Delgado T., Melero J. A. 1996; Identification of protein regions involved in the interaction of human respiratory syncytial virus phosphoprotein and nucleoprotein: significance for nucleocapsid assembly and formation of cytoplasmic inclusions. J Virol 70:801–808
    [Google Scholar]
  10. Grosfeld H., Hill M. G., Collins P. L. 1995; RNA replication by respiratory syncytial virus (RSV) is directed by the N, P and L proteins; transcription also occurs under these conditions but requires RSV superinfection for efficient synthesis of full-length mRNA. J Virol 69:5677–5686
    [Google Scholar]
  11. Hengst U., Kiefer P. 2000; Domains of human respiratory syncytial virus P protein essential for homodimerization and for binding to N and NS1 protein. Virus Genes 20:221–225
    [Google Scholar]
  12. Horsfall F. L., Hahn R. G. 1939; A pneumonia virus of Swiss mice. Proc Soc Exp Biol Med 40:684–686
    [Google Scholar]
  13. Horsfall F. L., Hahn R. G. 1940; A latent virus in normal mice capable of producing pneumonia in its natural host. J Exp Med 71:391–408
    [Google Scholar]
  14. Khattar S. K., Yunus A. S., Collins P. L., Samal S. K. 2000; Mutational analysis of the bovine respiratory syncytial virus nucleocapsid protein using a minigenome system: mutations that affect encapsidation, RNA synthesis, and interaction with the phosphoprotein. Virology 270:215–228
    [Google Scholar]
  15. Khattar S. K., Yunus A. S., Collins P. L., Samal S. K. 2001; Deletion and substitution analysis defines regions and residues within the phosphoprotein of bovine respiratory syncytial virus that affect transcription, RNA replication, and interaction with the nucleoprotein. Virology 285:253–269
    [Google Scholar]
  16. Lamb R. A., Kolakofsky D. 2001; Paramyxoviridae : the viruses and their replication. In Fields Virology , 4th edn. pp  1305–1340 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  17. Marriott A. C., Easton A. J. 1999; Reverse genetics of the Paramyxoviridae . Adv Virus Res 53:321–340
    [Google Scholar]
  18. Marriott A. C., Wilson S. D., Randhawa J. S., Easton A. J. 1999; A single amino acid substitution in the phosphoprotein of respiratory syncytial virus confers thermosensitivity in a reconstituted RNA polymerase system. J Virol 73:5162–5165
    [Google Scholar]
  19. Marriott A. C., Smith J. M., Easton A. J. 2001; Fidelity of leader and trailer sequence usage by the respiratory syncytial virus and avian pneumovirus replication complexes. J Virol 75:6265–6272
    [Google Scholar]
  20. Meric C., Spehner D., Mazarin V. 1994; Respiratory syncytial virus nucleocapsid protein (N) expressed in insect cells forms nucleocapsid-like structures. Virus Res 31:187–201
    [Google Scholar]
  21. Moss B., Elroy-Stein O., Mizukami T., Alexander W. A., Fuerst T. R. 1990; New mammalian expression vectors. Nature 348:91–92
    [Google Scholar]
  22. Murphy L. B., Loney C., Murray J., Bhella D., Ashton P., Yeo R. P. 2003; Investigations into the amino-terminal domain of the respiratory syncytial virus nucleocapsid protein reveal elements important for nucleocapsid formation and interaction with the phosphoprotein. Virology 307:143–153
    [Google Scholar]
  23. Murray J., Loney C., Murphy L. B., Graham S., Yeo R. P. 2001; Characterization of monoclonal antibodies raised against recombinant respiratory syncytial virus nucleocapsid (N) protein: identification of a region in the carboxy teminus of N involved in the interaction with P protein. Virology 289:252–261
    [Google Scholar]
  24. Pringle C. R., Eglin R. P. 1986; Murine pneumonia virus: seroepidemiological evidence of widespread human infection. J Gen Virol 67:975–982
    [Google Scholar]
  25. Samal S. K., Pastey M. K., McPhilips T. H., Mohanty S. B. 1993; Bovine respiratory syncytial virus nucleocapsid protein expressed in insect cells specifically interacts with the phosphoprotein and the M2 protein. Virology 193:470–473
    [Google Scholar]
  26. Slack M. S., Easton A. J. 1998; Characterization of the interaction of the human respiratory syncytial virus phosphoprotein and nucleocapsid protein using the two-hybrid system. Virus Res 55:167–176
    [Google Scholar]
  27. Yu Q., Hardy R. W., Wertz G. W. 1995; Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L proteins support replication of RS virus genomic RNA analogs and define minimal trans -acting requirements for RNA replication. J Virol 69:2412–2419
    [Google Scholar]
  28. Yunus A. S., Collins P. L., Samal S. K. 1998; Sequence analysis of a functional polymerase (L) gene of bovine respiratory syncytial virus: determination of minimal trans -acting requirements for RNA replication. J Gen Virol 79:2231–2238
    [Google Scholar]
  29. Zhou H., Cheng X., Jin H. 2003; Identification of amino acids that are critical to the processivity function of respiratory syncytial virus M2-1 protein. J Virol 77:5046–5053
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19370-0
Loading
/content/journal/jgv/10.1099/vir.0.19370-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error