1887

Abstract

The expression of the genes of varicella-zoster virus (VZV) is regulated by self-encoded viral as well as cellular transcription factors. A potential candidate with an ability to influence the transcription of VZV genes is USF (upstream stimulatory factor), which recognizes the consensus E-box motif. Quantitative RT-PCR and immunoblot assays indicate stable expression of both USF1 and USF2 throughout infection. It was also found that USF binds to a variety of E-boxes (consensus and closely related motifs) within the promoters of ORF 8/9 (two elements), ORF 22 and ORF 67. Co-immunoprecipitation experiments and His-tag protein affinity pull-down assays indicate that a direct physical interaction occurs between USF and the major virus transactivator IE62. To study the general effects of USF in the replication of VZV, a cell line expressing a dominant–negative form of USF (A-USF), which inhibits binding of USF to its recognition sites, was created. A significant decrease in virus replication was detected when this cell line was infected with cell-free virus, indicating that USF is an important cellular factor that regulates the transcription of VZV genes.

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2003-11-01
2020-01-22
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References

  1. Andrews, N. C. & Faller, D. V. ( 1991; ). A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res 19, 2499.[CrossRef]
    [Google Scholar]
  2. Angel, P. & Karin, M. ( 1991; ). The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim Biophys Acta 1072, 129–157.
    [Google Scholar]
  3. Arvin, A. M., Kinney-Thomas, E., Shriver, K., Grose, C., Koropchak, C. M., Scranton, E., Wittek, A. E. & Diaz, P. S. ( 1986; ). Immunity to varicella-zoster viral glycoproteins, gp I (gp 90/58) and gp III (gp 118), and to a nonglycosylated protein, p 170. J Immunol 137, 1346–1351.
    [Google Scholar]
  4. Ayer, D. E., Kretzner, L. & Eisenman, R. N. ( 1993; ). Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity. Cell 72, 211–222.[CrossRef]
    [Google Scholar]
  5. Beckmann, H., Su, L. K. & Kadesch, T. ( 1990; ). TFE3: a helix–loop–helix protein that activates transcription through the immunoglobulin enhancer muE3 motif. Genes Dev 4, 167–179.[CrossRef]
    [Google Scholar]
  6. Blackwell, T. K., Kretzner, L., Blackwood, E. M., Eisenman, R. N. & Weintraub, H. ( 1990; ). Sequence-specific DNA binding by the c-Myc protein. Science 250, 1149–1151.[CrossRef]
    [Google Scholar]
  7. Blackwood, E. M. & Eisenman, R. N. ( 1991; ). Max: a helix–loop–helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science 251, 1211–1217.[CrossRef]
    [Google Scholar]
  8. Boldogh, I., AbuBakar, S. & Albrecht, T. ( 1990; ). Activation of proto-oncogenes: an immediate early event in human cytomegalovirus infection. Science 247, 561–564.[CrossRef]
    [Google Scholar]
  9. Carr, C. S. & Sharp, P. A. ( 1990; ). A helix–loop–helix protein related to the immunoglobulin E box-binding proteins. Mol Cell Biol 10, 4384–4388.
    [Google Scholar]
  10. Carthew, R. W., Chodosh, L. A. & Sharp, P. A. ( 1985; ). An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell 43, 439–448.[CrossRef]
    [Google Scholar]
  11. Disney, G. H., McKee, T. A., Preston, C. M. & Everett, R. D. ( 1990; ). The product of varicella-zoster virus gene 62 autoregulates its own promoter. J Gen Virol 71, 2999–3003.[CrossRef]
    [Google Scholar]
  12. Forghani, B., Mahalingam, R., Vafai, A., Hurst, J. W. & Dupuis, K. W. ( 1990; ). Monoclonal antibody to immediate early protein encoded by varicella-zoster virus gene 62. Virus Res 16, 195–210.[CrossRef]
    [Google Scholar]
  13. Förster, E. ( 1994; ). An improved general method to generate internal standards for competitive PCR. Biotechniques 16, 18–20.
    [Google Scholar]
  14. Halazonetis, T. D. & Kandil, A. N. ( 1991; ). Determination of the c-MYC DNA-binding site. Proc Natl Acad Sci U S A 88, 6162–6166.[CrossRef]
    [Google Scholar]
  15. He, H., Boucaud, D., Hay, J. & Ruyechan, W. T. ( 2001; ). Cis and trans elements regulating expression of the varicella zoster virus gI gene. Arch Virol Suppl 17, 57–70.
    [Google Scholar]
  16. Inchauspe, G. & Ostrove, J. M. ( 1989; ). Differential regulation by varicella-zoster virus (VZV) and herpes simplex virus type-1 trans-activating genes. Virology 173, 710–714.[CrossRef]
    [Google Scholar]
  17. Ito, H., Sommer, M. H., Zerboni, L., He, H., Boucaud, D., Hay, J., Ruyechan, W. & Arvin, A. M. ( 2003; ). Promoter sequences of varicella-zoster virus glycoprotein I targeted by cellular transactivating factors Sp1 and USF determine virulence in skin and T cells in SCIDhu mice in vivo. J Virol 77, 489–498.[CrossRef]
    [Google Scholar]
  18. Jang, K. L., Pulverer, B., Woodgett, J. R. & Latchman, D. S. ( 1991; ). Activation of the cellular transcription factor AP-1 in herpes simplex virus infected cells is dependent on the viral immediate–early protein ICP0. Nucleic Acids Res 19, 4879–4883.[CrossRef]
    [Google Scholar]
  19. Kenyon, T. K., Lynch, J., Hay, J., Ruyechan, W. T. & Grose, C. ( 2001; ). Varicella-zoster virus ORF47 protein serine kinase: characterization of a cloned, biologically active phosphotransferase and two viral substrates, ORF62 and ORF63. J Virol 75, 8854–8858.[CrossRef]
    [Google Scholar]
  20. Kim, J. B. & Spiegelman, B. M. ( 1996; ). ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev 10, 1096–1107.[CrossRef]
    [Google Scholar]
  21. Kinchington, P. R., Hougland, J. K., Arvin, A. M., Ruyechan, W. T. & Hay, J. ( 1992; ). The varicella-zoster virus immediate–early protein IE62 is a major component of virus particles. J Virol 66, 359–366.
    [Google Scholar]
  22. Kinchington, P. R., Vergnes, J. P., Defechereux, P., Piette, J. & Turse, S. E. ( 1994; ). Transcriptional mapping of the varicella-zoster virus regulatory genes encoding open reading frames 4 and 63. J Virol 68, 3570–3581.
    [Google Scholar]
  23. Krylov, D., Mikhailenko, I. & Vinson, C. ( 1994; ). A thermodynamic scale for leucine zipper stability and dimerization specificity: E and G interhelical interactions. EMBO J 13, 2849–2861.
    [Google Scholar]
  24. Krylov, D., Kasai, K., Echlin, D. R., Taparowsky, E. J., Arnheiter, H. & Vinson, C. ( 1997; ). A general method to design dominant negatives to B-HLHZip proteins that abolish DNA binding. Proc Natl Acad Sci U S A 94, 12274–12279.[CrossRef]
    [Google Scholar]
  25. Lania, L., Majello, B. & De Luca, P. ( 1997; ). Transcriptional regulation by the Sp family proteins. Int J Biochem Cell Biol 29, 1313–1323.[CrossRef]
    [Google Scholar]
  26. Lynch, J. Kenyon T. K., Grose, C., Hay, J. & Ruyechan, W. T. ( 2002; ). Physical and functional interaction between the varicella zoster virus IE62 and IE63 proteins. Virology 302, 71–82.[CrossRef]
    [Google Scholar]
  27. MacGregor, D., Li, L. H. & Ziff, E. B. ( 1996; ). Dominant negative mutants of Myc inhibit cooperation of both Myc and adenovirus serotype-5 E1a with Ras. J Cell Physiol 167, 95–105.[CrossRef]
    [Google Scholar]
  28. McKee, T. A., Disney, G. H., Everett, R. D. & Preston, C. M. ( 1990; ). Control of expression of the varicella-zoster virus major immediate early gene. J Gen Virol 71, 897–906.[CrossRef]
    [Google Scholar]
  29. Meier, J. L., Luo, X., Sawadogo, M. & Straus, S. E. ( 1994; ). The cellular transcription factor USF cooperates with varicella-zoster virus immediate–early protein 62 to symmetrically activate a bidirectional viral promoter. Mol Cell Biol 14, 6896–6906.
    [Google Scholar]
  30. Miyamoto, N. G., Moncollin, V., Egly, J. M. & Chambon, P. ( 1985; ). Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter. EMBO J 4, 3563–3570.
    [Google Scholar]
  31. Moriuchi, M., Moriuchi, H., Straus, S. E. & Cohen, J. I. ( 1994; ). Varicella-zoster virus (VZV) virion-associated transactivator open reading frame 62 protein enhances the infectivity of VZV DNA. Virology 200, 297–300.[CrossRef]
    [Google Scholar]
  32. Moriuchi, H., Moriuchi, M. & Cohen, J. I. ( 1995; ). Proteins and cis-acting elements associated with transactivation of the varicella-zoster virus (VZV) immediate–early gene 62 promoter by VZV open reading frame 10 protein. J Virol 69, 4693–4701.
    [Google Scholar]
  33. Murre, C., McCaw, P. S. & Baltimore, D. ( 1989; ). A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and Myc proteins. Cell 56, 777–783.[CrossRef]
    [Google Scholar]
  34. Ng, T. I., Keenan, L., Kinchington, P. R. & Grose, C. ( 1994; ). Phosphorylation of varicella-zoster virus open reading frame (ORF) 62 regulatory product by viral ORF 47-associated protein kinase. J Virol 68, 1350–1359.
    [Google Scholar]
  35. Perera, L. P. ( 2000; ). The TATA motif specifies the differential activation of minimal promoters by varicella zoster virus immediate–early regulatory protein IE62. J Biol Chem 275, 487–496.[CrossRef]
    [Google Scholar]
  36. Perera, L. P., Mosca, J. D., Sadeghi-Zadeh, M., Ruyechan, W. T. & Hay, J. ( 1992; ). The varicella-zoster virus immediate early protein, IE62, can positively regulate its cognate promoter. Virology 191, 346–354.[CrossRef]
    [Google Scholar]
  37. Prendergast, G. C. & Ziff, E. B. ( 1991; ). Methylation-sensitive sequence-specific DNA binding by the c-Myc basic region. Science 251, 186–189.[CrossRef]
    [Google Scholar]
  38. Prendergast, G. C., Lawe, D. & Ziff, E. B. ( 1991; ). Association of Myn, the murine homolog of Max, with c-Myc stimulates methylation-sensitive DNA binding and Ras cotransformation. Cell 65, 395–407.[CrossRef]
    [Google Scholar]
  39. Qyang, Y., Luo, X., Lu, T., Ismail, P. M., Krylov, D., Vinson, C. & Sawadogo, M. ( 1999; ). Cell-type-dependent activity of the ubiquitous transcription factor USF in cellular proliferation and transcriptional activation. Mol Cell Biol 19, 1508–1517.
    [Google Scholar]
  40. Rahaus, M. & Wolff, M. H. ( 1999; ). Influence of different cellular transcription factors on the regulation of varicella-zoster virus glycoproteins E (gE) and I (gI) UTR's activity. Virus Res 62, 77–88.[CrossRef]
    [Google Scholar]
  41. Rahaus, M. & Wolff, M. H. ( 2000; ). Transcription factor Sp1 is involved in the regulation of varicella-zoster virus glycoprotein E. Virus Res 69, 69–81.[CrossRef]
    [Google Scholar]
  42. Rahaus, M. & Wolff, M. H. ( 2003; ). Reciprocal effects of varicella-zoster virus (VZV) and Ap1: activation of Jun, Fos and ATF-2 after VZV infection and their importance for the regulation of viral genes. Virus Res 92, 9–21.[CrossRef]
    [Google Scholar]
  43. Rolfs, A., Schuller, I., Finckh, U. & Weber-Rolfs, I. ( 1992; ). Reverse transcription/PCR (RT-PCR). In PCR: Clinical Diagnostics and Research, pp. 99–102. Edited by A. Rolfs. Berlin: Springer-Verlag.
  44. Ruyechan, W. T. & Hay, J. ( 2000; ). DNA replication. In Varicella-Zoster Virus: Virology and Clinical Management, pp. 51–73. Edited by A. M. Arvin & A. A. Gershon. Cambridge: Cambridge University Press.
  45. Ruyechan, W. T., Peng, H., Yang, M. & Hay, J. ( 2003; ). Cellular factors and IE62 activation of VZV promoters. J Med Virol 70 (Suppl. 1), S90–S94.[CrossRef]
    [Google Scholar]
  46. Sadzot-Delvaux, C., Baudoux, L., Defechereux, P., Piette, J. & Rentier, B. ( 1999; ). Overview of the replication cycle of varicella-zoster virus. In Varicella-Zoster Virus: Molecular Biology, Pathogenesis, and Clinical Aspects, pp. 21–42. Edited by M. H. Wolff, S. Schünemann & A. Schmidt. Basel: Karger.
  47. Sawadogo, M. ( 1988; ). Multiple forms of the human gene-specific transcription factor USF. II. DNA binding properties and transcriptional activity of the purified HeLa USF. J Biol Chem 263, 11994–12001.
    [Google Scholar]
  48. Sawadogo, M. & Roeder, R. G. ( 1985; ). Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region. Cell 43, 165–175.[CrossRef]
    [Google Scholar]
  49. Sawadogo, M., Van Dyke, M. W., Gregor, P. D. & Roeder, R. G. ( 1988; ). Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei. J Biol Chem 263, 11985–11993.
    [Google Scholar]
  50. Sirito, M., Lin, Q., Maity, T. & Sawadogo, M. ( 1994; ). Ubiquitous expression of the 43- and 44-kDa forms of transcription factor USF in mammalian cells. Nucleic Acids Res 22, 427–433.[CrossRef]
    [Google Scholar]
  51. Spengler, M. L., Ruyechan, W. T. & Hay, J. ( 2000; ). Physical interaction between two varicella zoster virus gene regulatory proteins, IE4 and IE62. Virology 272, 375–381.[CrossRef]
    [Google Scholar]
  52. Suske, G. ( 1999; ). The Sp-family of transcription factors. Gene 238, 291–300.[CrossRef]
    [Google Scholar]
  53. Tyler, J. K. & Everett, R. D. ( 1993; ). The DNA binding domain of the varicella-zoster virus gene 62 protein interacts with multiple sequences which are similar to the binding site of the related protein of herpes simplex virus type 1. Nucleic Acids Res 21, 513–522.[CrossRef]
    [Google Scholar]
  54. Waterboer, T., Rahaus, M. & Wolff, M. H. ( 2002; ). Varicella-zoster virus (VZV) mediates a delayed host shutoff independent of open reading frame (ORF) 17 expression. Virus Genes 24, 49–56.[CrossRef]
    [Google Scholar]
  55. Zervos, A. S., Gyuris, J. & Brent, R. ( 1993; ). Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell 72, 223–232.[CrossRef]
    [Google Scholar]
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