1887

Abstract

The endothelial cell line ECV304, derived from human umbilical cord and identified to be susceptible to dengue virus type 2 (DEN-2) infection, was used to study the molecular mechanism of DEN-2 binding to endothelial cells. DEN-2 was found by virus overlay protein-binding assays (VOPBAs) to bind to three ECV304 cell membrane proteins with molecular masses of 29, 34 and 43 kDa. Only a single protein of 29 kDa was observed when VOPBAs were carried out using preparations of trypsin-treated ECV304 cells. Pre-incubation of live ECV304 cells in culture or cell membrane proteins in modified VOPBAs with the recombinant DEN-2 envelope glycoprotein (rEgp) inhibited DEN-2 infection and blocked virus binding to the three proteins identified. These results indicate that DEN-2 rEgp could bind to three proteins on the surface of ECV304 cells. This virus–cell interaction may be associated with the receptor complex specific for DEN-2 infection of endothelial cells.

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/content/journal/jgv/10.1099/vir.0.19308-0
2003-11-01
2020-05-28
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References

  1. Anderson R., King A. D., Innis B. L.. 1992; Correlation of E protein binding with cell susceptibility to dengue 4 virus infection. J Gen Virol73:2155–2159
    [Google Scholar]
  2. Bielefeldt-Ohmann H., Meyer M., Fitzpatrick D. R., Mackenzie J. S.. 2001; Dengue virus binding to human leukocyte cell lines: receptor usage differs between cell types and virus strains. Virus Res73:81–89
    [Google Scholar]
  3. Bonner S. M., O'Sullivan M. A.. 1998; Endothelial cell monolayers as a model system to investigate dengue shock syndrome. J Virol Methods71:159–167
    [Google Scholar]
  4. Chen Y., Maguire T., Marks R. M.. 1996; Demonstration of binding of dengue virus envelope protein to target cells. J Virol70:8765–8772
    [Google Scholar]
  5. Chen Y., Magurie T., Hileman R. E., Fromm J. R., Esko J. D., Linhardt R. J., Marks R. M.. 1997; Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nat Med3:866–871
    [Google Scholar]
  6. Chen Y. C., Wang S. Y., King C. C.. 1999; Bacterial lipopolysaccharide inhibits dengue virus infection of primary human monocytes/macrophages by blockade of virus entry via a CD14-dependent mechanism. J Virol73:2650–2657
    [Google Scholar]
  7. Daughaday C. C., Brandt W. E., McCown J. M., Russell P. K.. 1981; Evidence for two mechanisms of dengue virus infection of adherent human monocytes: trypsin-sensitive virus receptors and trypsin-resistant immune complex receptors. Infect Immun32:469–473
    [Google Scholar]
  8. Huang Y. H., Lei H. Y., Liu H. S., Lin H. S., Liu C. C., Yeh T. M.. 2002; Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production. Am J Trop Med Hyg63:71–76
    [Google Scholar]
  9. Killen H., O'Sullivan M. A.. 1993; Detection of dengue virus by in situ hybridization. J Virol Methods41:135–146
    [Google Scholar]
  10. Littaua R., Kurane I., Ennis F. A.. 1990; Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. J Immunol144:3183–3186
    [Google Scholar]
  11. Marianneau P., Megret F., Olivier R., Morens D. M., Deubel V.. 1996; Dengue 1 virus binding to human hepatoma HepG2 and simian Vero cell surfaces differs. J Gen Virol77:2547–2554
    [Google Scholar]
  12. Salas-Benito J. S., del Angel R. M.. 1997; Identification of two surface proteins from C6/36 cells that bind dengue type 4 virus. J Virol71:7246–7252
    [Google Scholar]
  13. Shieh M. T., WuDunn D., Montgomery R. I., Esko J. D., Spear P. G.. 1992; Cell surface receptors for herpes simplex virus are heparan sulfate proteoglycans. J Cell Biol116:1273–1281
    [Google Scholar]
  14. Wei H. Y., Jiang L. F., Xue Y. H., Fang D. Y., Guo H. Y.. 2003; Secreted expression of dengue virus type 2 full-length envelope glycoprotein in Pichia pastoris . J Virol Methods109:17–23
    [Google Scholar]
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