1887

Abstract

A virus isolated from dead bats collected near Kampot, Cambodia, was identified as a member of the family by electron microscopy. The only bunyavirus previously isolated from species bats in South-East Asia is Kaeng Khoi (KK) virus (genus ), detected in Thailand over 30 years earlier and implicated as a public health problem. Using RT-PCR, nucleotide sequences from the M RNA segment of several virus isolates from the Cambodian bats were found to be almost identical and to differ from those of the prototype KK virus by only 2·6–3·2 %, despite the temporal and geographic separation of the viruses. These results identify the Cambodian bat viruses as KK virus, extend the known virus geographic range and document the first KK virus isolation in 30 years. These genetic data, together with earlier serologic data, show that KK viruses represent a distinct group within the genus .

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2003-10-01
2020-08-11
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402
    [Google Scholar]
  2. Bowen M. D., Trappier S. G., Sanchez A. J.. 8 other authors 2001; A reassortant bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia. Virology291:185–190
    [Google Scholar]
  3. Brockus C. L., Grimstad P. R.. 2001; Comparative analysis of G1 glycoprotein-coding sequences of Cache Valley virus (Bunyaviridae: Bunyavirus) isolates. Virus Genes22:133–139
    [Google Scholar]
  4. Gonzalez-Scarano F., Bupp K., Nathanson N.. 1996; Pathogenesis of diseases caused by viruses of the Bunyavirus genus. In The Bunyaviridae pp 227–251 Edited by Elliott R. M.. New York: Plenum Press;
    [Google Scholar]
  5. Hasegawa M., Kishino H., Yano T.. 1985; Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol22:160–174
    [Google Scholar]
  6. Huang C., Thompson W. H., Karabatsos N., Grady L., Campbell W. P.. 1997; Evidence that fatal human infections with La Crosse virus may be associated with a narrow range of genotypes. Virus Res48:143–148
    [Google Scholar]
  7. Karabatsos N.. (editor) 1985; International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates , 3rd edn. San Antonio: American Society for Tropical Medicine and Hygiene;
    [Google Scholar]
  8. Mullenhauer H. H.. 1964; Plastic embedding mixtures for use in electron microscopy. Stain Technol39:111–114
    [Google Scholar]
  9. Neill W. A.. 1985; In International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates . , 3rd edn. pp 533–534 Edited by Karabatsos N.. San Antonio: American Society for Tropical Medicine and Hygiene;
  10. Nichol S. T.. 2001; Bunyaviruses. In Fields Virology vol. 2 pp 1603–1634 Edited by Knipe D. M., Howley P. M.. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  11. Olson J. G., Rupprecht C., Rollin P. E., An U. S., Niezgoda M., Clemins T., Walston J., Ksiazek T. G.. 2002; Antibodies to Nipah-like virus in bats ( Pteropus lylei ), Cambodia. Emerg Infect Dis8:987–988
    [Google Scholar]
  12. Whitfield S. G., Fekadu M., Shaddock J. H., Niezgoda M., Warner C. K., Messenger S. L., the Rabies Working Group. 2001; A comparative study of the fluorescent antibody test for rabies diagnosis in fresh and formalin-fixed brain tissue specimens. J Virol Methods95:145–151
    [Google Scholar]
  13. Williams J. E., Imlarp S., Top F. H. Jr, Cavanaugh D. C., Russell P. K.. 1976; Kaeng Khoi virus from naturally infected bedbugs ( Cimicidae ) and immature free-tailed bats. Bull World Health Organ53:365–369
    [Google Scholar]
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