1887

Abstract

Open reading frame 94 () of single nucleocapsid nucleopolyhedrovirus (HaSNPV) is 1086 bp long and a homologue of multiple NPV ORF109. The gene is conserved among all baculoviruses whose genomes have been completely sequenced so far and is thus considered a baculovirus core gene. transcripts were detected from 24 to 96 h post-infection (p.i.) of HzAM1 cells with HaSNPV. Polyclonal antiserum raised to a GST–HA94 fusion protein recognized a 43 kDa protein, HA94, in infected cell lysates from 36 to 96 h p.i., suggesting that is a late gene. Western blot analysis of proteins present in budded virus and occlusion-derived virus (ODV) showed that encodes a structural component of ODV. When ODVs were fractionated further into nucleocapsid and envelope components, Western blot analysis indicated that the encoded protein was associated with both the nucleocapsid and the envelope. In summary, data available indicated that encodes a novel ODV-specific protein of HaSNPV, designated ODV-EC43.

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2003-11-01
2020-01-21
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