1887

Abstract

RNA sequences required for assembly into rod-shaped virions of RNA-1 and RNA-2 of (PCV) were mapped by testing the ability of different RNA-1 and -2 deletion mutants to be encapsidated in an RNase-resistant form. Encapsidation of RNA-1 was found to require a sequence domain in the 5′-proximal part of the P15 gene, the 3′-proximal gene of RNA-1. On the other hand, the subgenomic RNA which encodes P15 was not encapsidated, suggesting that other features of RNA-1 are important as well. Two sequences which could drive encapsidation of RNA-2 deletion mutants were located. One was in the 5′-proximal coat protein gene and the other in the P14 gene near the RNA 3′ terminus. There were no obvious sequence homologies between the different assembly initiation sequences.

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2003-09-01
2020-09-25
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References

  1. Culver J. N.. 2002; Tobacco mosaic virus assembly and disassembly: determinants in pathogenicity and resistance. Annu Rev Phytopathol40:287–308
    [Google Scholar]
  2. Dunoyer P., Herzog E., Hemmer O., Ritzenthaler C., Fritsch C.. 2001; Peanut clump virus RNA-1-encoded P15 regulates viral RNA accumulation but is not abundant at viral RNA replication sites. J Virol75:1941–1948
    [Google Scholar]
  3. Dunoyer P., Pfeffer S., Fritsch C., Hemmer O., Voinnet O., Richards K. E.. 2002a; Identification, subcellular localization and some properties of a cysteine-rich suppressor of gene silencing encoded by peanut clump virus. Plant J29:555–567
    [Google Scholar]
  4. Dunoyer P., Ritzenthaler C., Hemmer O., Michler P., Fritsch C.. 2002b; Intracellular localization of the peanut clump virus replication complex in tobacco BY-2 protoplasts containing green fluorescent protein-labeled endoplasmic reticulum or Golgi apparatus. J Virol76:865–874
    [Google Scholar]
  5. Erhardt M., Stussi-Garaud C., Guilley H., Richards K. E., Jonard G., Bouzoubaa S.. 1999; The first triple gene block protein of peanut clump virus localizes to the plasmodesmata during virus infection. Virology264:220–229
    [Google Scholar]
  6. Fritsch C., Dollet M.. 2000; Genus Pecluvirus . In Virus Taxonomy . Seventh Report of the International Committee on Taxonomy of Viruses . pp 913–917 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B.. San Diego: Academic Press;
  7. Fukuda M., Okada Y., Otsuki Y., Takebe I.. 1980; The site of initiation of rod assembly on the RNA of a tomato and a cowpea strain of tobacco mosaic virus. Virology101:493–502
    [Google Scholar]
  8. Guilley H., Jonard G., Richards K. E., Hirth L.. 1975; Sequence of a specifically encapsidated RNA fragment originating from the tobacco-mosaic-virus coat-protein cistron. Eur J Biochem54:135–144
    [Google Scholar]
  9. Hemmer O., Oncino C., Fritsch C.. 1993; Efficient replication of the in vitro transcripts from cloned cDNA of tomato black ring virus satellite RNA requires the 48 K satellite RNA-encoded protein. Virology194:800–806
    [Google Scholar]
  10. Herzog E., Guilley H., Manohar S. K., Dollet M., Richards K., Fritsch C., Jonard G.. 1994; Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. J Gen Virol75:3147–3155
    [Google Scholar]
  11. Herzog E., Guilley H., Fritsch C.. 1995; Translation of the second gene of peanut clump virus RNA-2 occurs by leaky scanning in vitro . Virology208:215–225
    [Google Scholar]
  12. Herzog E., Hemmer O., Hauser S., Meyer G., Bouzoubaa S., Fritsch C.. 1998; Identification of genes involved in replication and movement of peanut clump virus. Virology248:312–322
    [Google Scholar]
  13. Ho S. N., Junt H. D., Horton R. M., Puller J. K., Pease L. R.. 1989; Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene77:51–59
    [Google Scholar]
  14. Lee Y. S., Lin B. Y., Hsu Y. H., Chang B. Y., Lin N. S.. 1998; Subgenomic RNAs of bamboo mosaic potexvirus-V isolate are packaged into virions. J Gen Virol79:1825–1832
    [Google Scholar]
  15. Manohar S. K., Guilley H., Dollet M., Richards K., Jonard G.. 1993; Nucleotide sequence and genetic organization of peanut clump virus RNA 2 and partial characterization of deleted forms. Virology195:33–41
    [Google Scholar]
  16. Matsuda D., Dunoyer P., Hemmer O., Fritsch C., Dreher T. W.. 2000; The valine anticodon and valylatability of peanut clump virus RNAs are not essential but provide a modest competitive advantage in plants. J Virol74:8720–8725
    [Google Scholar]
  17. Nagata T., Nemoto Y., Hasezawa S.. 1992; Tobacco BY-2 cell line as the “HeLa” cell in the cell biology of higher plants. Int Rev Cytol132:1–30
    [Google Scholar]
  18. Sit T. L., Leclerc D., AbouHaidar M. G.. 1994; The minimal 5′ sequence for in vitro initiation of papaya mosaic potexvirus assembly. Virology199:238–242
    [Google Scholar]
  19. Turner D. R., Joyce L. E., Butler P. J.. 1988; The tobacco mosaic virus assembly origin RNA. Functional characteristics defined by directed mutagenesis. J Mol Biol203:531–547
    [Google Scholar]
  20. Verchot J., Angell S. M., Baulcombe D. C.. 1998; In vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs. J Virol72:8316–8320
    [Google Scholar]
  21. Wu X., Shaw J. G.. 1998; Evidence that assembly of a potyvirus begins near the 5′ terminus of the viral RNA. J Gen Virol79:1525–1529
    [Google Scholar]
  22. Zimmern D.. 1977; The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell11:463–482
    [Google Scholar]
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