1887

Abstract

The susceptibility of prion protein gene ()-null cells to coxsackievirus B3 (CVB3) was investigated. Primary cultures of murine brain cells were more sensitive to CVBs than corresponding cells from wild-type mice. The viral susceptibility of a -null cell line (HpL3-4) derived from the murine hippocampus was compared with that of two established cell lines (HeLa and HEp-2) that are widely employed for CVB3 studies. After infection with CVB3, HpL3-4 cells showed a very rapid and complete cytopathic effect (CPE). CPE developed earlier and viruses replicated at higher titres in HpL3-4 cells compared with HeLa and HEp-2 cells. Under a semi-solid medium, plaques developed rapidly in CVB3-infected HpL3-4 cells. To confirm the effect of on virus infection, a cell line and a -transfected neuronal cell line were analysed. The replication and release of infectious particles of CVB3 in cells were significantly more effective than those of the -transfected cell line. Levels of type I interferon (IFN) after CVB3 infection were higher in the -transfected cell line than in cells, whereas apoptotic cells were more obvious in the cells than in those of the -transfected cell line. These findings suggest that the absence of retards the induction of CVB3-induced IFNs, resulting in an enhanced CVB3 production and apoptotic cell death. Furthermore, our data indicate that the HpL3-4 cell line may provide a novel and sensitive system for isolation of CVB3 from clinical specimens.

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2003-12-01
2020-01-29
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