1887

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Volume 84, Issue 7

No role for Epstein–Barr virus in Dutch hepatocellular carcinoma: a study at the DNA, RNA and protein levels Free

Abstract

Epstein–Barr virus (EBV) has been suggested to play a role in hepatocellular carcinoma (HCC). However, reports on detailed EBV transcript analyses in HCCs are limited. It was shown recently that expression of the transforming (HI ) gene of EBV is restricted to latently EBV-infected epithelial malignancies, i.e. nasopharyngeal carcinoma and gastric carcinoma. The aim of this study was to test the presence of EBV in Dutch HCCs. A semiquantitative DNA PCR-enzyme immunoassay (PCR-EIA) for the HI W fragment of EBV was used to assess the presence of EBV in frozen and paraffin-embedded tissues of 16 HCCs. In addition, several RNA detection techniques, i.e. nucleic acid sequence-based amplification (NASBA), RT-PCR, RNA hybridization (RISH) and immunohistochemistry (IHC), were applied. Five of 16 HCCs and two of four hepatitis C virus hepatitis samples were weakly positive for EBV DNA by PCR-EIA. Using sensitive RNA transcription techniques, no transcripts were found for , EBNA-1 and BARTs (HI A rightward transcripts) in any of the liver tissues tested. In addition, RISH for EBER1/2 and BARTs and IHC for EBNA-1, LMP-1 and ZEBRA, performed on the paraffin-embedded tissue of the PCR-EIA-positive cases and on adjacent non-neoplastic liver tissues, were negative. The absence of epithelial-specific transcripts and other EBV transcripts and proteins in the EBV DNA PCR-positive cases argues strongly against a role for EBV in HCC.

  • Received:
  • Accepted:
  • Published Online:
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2003-07-01
2024-04-19
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No role for Epstein–Barr virus in Dutch hepatocellular carcinoma: a study at the DNA, RNA and protein levels
J. Gen. Virol. 84, 1863 (2003); https://doi.org/10.1099/vir.0.19217-0
/content/journal/jgv/10.1099/vir.0.19217-0
/content/journal/jgv/10.1099/vir.0.19217-0
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