1887

Abstract

Epstein–Barr virus (EBV) has been suggested to play a role in hepatocellular carcinoma (HCC). However, reports on detailed EBV transcript analyses in HCCs are limited. It was shown recently that expression of the transforming (HI ) gene of EBV is restricted to latently EBV-infected epithelial malignancies, i.e. nasopharyngeal carcinoma and gastric carcinoma. The aim of this study was to test the presence of EBV in Dutch HCCs. A semiquantitative DNA PCR-enzyme immunoassay (PCR-EIA) for the HI W fragment of EBV was used to assess the presence of EBV in frozen and paraffin-embedded tissues of 16 HCCs. In addition, several RNA detection techniques, i.e. nucleic acid sequence-based amplification (NASBA), RT-PCR, RNA hybridization (RISH) and immunohistochemistry (IHC), were applied. Five of 16 HCCs and two of four hepatitis C virus hepatitis samples were weakly positive for EBV DNA by PCR-EIA. Using sensitive RNA transcription techniques, no transcripts were found for , EBNA-1 and BARTs (HI A rightward transcripts) in any of the liver tissues tested. In addition, RISH for EBER1/2 and BARTs and IHC for EBNA-1, LMP-1 and ZEBRA, performed on the paraffin-embedded tissue of the PCR-EIA-positive cases and on adjacent non-neoplastic liver tissues, were negative. The absence of epithelial-specific transcripts and other EBV transcripts and proteins in the EBV DNA PCR-positive cases argues strongly against a role for EBV in HCC.

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2003-07-01
2020-01-22
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References

  1. Bijl, J., van Oostveen, J. W., Kreike, M. & 7 other authors ( 1996; ). Expression of HOXC4, HOXC5, and HOXC6 in human lymphoid cell lines, leukemias, and benign and malignant lymphoid tissue. Blood 87, 1737–1745.
    [Google Scholar]
  2. Bonnet, M., Guinebretiere, J. M., Kremmer, E., Grunewald, V., Benhamou, E., Contesso G. & Joab I. ( 1999; ). Detection of Epstein–Barr virus in invasive breast cancers. J Natl Cancer Inst 91, 1376–1381.[CrossRef]
    [Google Scholar]
  3. Brink, A. A., Oudejans, J. J., Jiwa, M., Walboomers, J. M., Meijer, C. J. L. M. & van den Brule, A. J. C. ( 1997; ). Multiprimed cDNA synthesis followed by PCR is the most suitable method for Epstein–Barr virus transcript analysis in small lymphoma biopsies. Mol Cell Probes 11, 39–47.[CrossRef]
    [Google Scholar]
  4. Brink, A. A., Vervoort, M. B., Middeldorp, J. M., Meijer, C. J. L. M. & van den Brule, A. J. C. ( 1998; ). Nucleic acid sequence-based amplification, a new method for analysis of spliced and unspliced Epstein–Barr virus latent transcripts, and its comparison with reverse transcriptase PCR. J Clin Microbiol 36, 3164–3169.
    [Google Scholar]
  5. Brink, A. A., van den Brule, A. J. C., van Diest, P. & Meijer, C. J. L. M. ( 2000; ). Re: Detection of Epstein–Barr virus in invasive breast cancers. J Natl Cancer Inst 92, 655–656.[CrossRef]
    [Google Scholar]
  6. Chen, H., Smith, P., Ambinder, R. F. & Hayward, S. D. ( 1999; ). Expression of Epstein–Barr virus BamHI-A rightward transcripts in latently infected B cells from peripheral blood. Blood 93, 3026–3032.
    [Google Scholar]
  7. Chu, P. G., Chen, Y. Y., Chen, W. G. & Weiss, L. M. ( 2001a; ). No direct role for Epstein–Barr virus in American hepatocellular carcinoma. Am J Pathol 159, 1287–1292.[CrossRef]
    [Google Scholar]
  8. Chu, P. G., Chang, K. L., Chen, Y. Y., Chen, W. G. & Weiss, L. M. ( 2001b; ). No significant association of Epstein–Barr virus infection with invasive breast carcinoma. Am J Pathol 159, 571–578.[CrossRef]
    [Google Scholar]
  9. Cruz, I., van den Brule, A. J. C., Brink, A. A., Snijders, P. J., Walboomers, J. M., van der Waal, I. & Meijer, C. J. L. M. ( 2000; ). No direct role for Epstein–Barr virus in oral carcinogenesis: a study at the DNA, RNA and protein levels. Int J Cancer 86, 356–361.[CrossRef]
    [Google Scholar]
  10. Deamant, F. D., Albujar, P. F., Chen, Y. Y. & Weiss, L. M. ( 1993; ). Epstein–Barr virus distribution in nonneoplastic lymph nodes. Mod Pathol 6, 729–732.
    [Google Scholar]
  11. de Roda Husman, A. M., Walboomers, J. M., van den Brule, A. J. C., Meijer, C. J. L. M. & Snijders, P. J. F. ( 1995; ). The use of general primer GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 76, 1057–1062.[CrossRef]
    [Google Scholar]
  12. El-Serag, H. B. & Mason, A. C. ( 1999; ). Rising incidence of hepatocellular carcinoma in the United States. N Engl J Med 340, 745–750.[CrossRef]
    [Google Scholar]
  13. Grässer, F. A., Murray, P. G., Kremmer, E. & 7 other authors ( 1994; ). Monoclonal antibodies directed against the Epstein–Barr virus-encoded nuclear antigen 1 (EBNA1): immunohistologic detection of EBNA1 in the malignant cells of Hodgkin's disease. Blood 84, 3792–3798.
    [Google Scholar]
  14. Hayes, D. P., Brink, A. A., Vervoort, M. B., Middeldorp, J. M., Meijer, C. J. L. M. & van den Brule, A. J. C. ( 1999; ). Expression of Epstein–Barr virus (EBV) transcripts encoding homologues to important human proteins in diverse EBV-associated diseases. Mol Pathol 52, 97–103.[CrossRef]
    [Google Scholar]
  15. IARC. ( 1994; ). Hepatitis Viruses. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, vol. 59. Lyon: IARC.
  16. IARC. ( 1998; ). Alcohol Drinking. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, vol. 44. Lyon: IARC.
  17. Jiwa, N. M., Kanavaros, P., van der Valk, P., Walboomers, J. M., Horstman, A., Vos, W., Mullink, H. & Meijer, C. J. L. M. ( 1993; ). Expression of c-myc and bcl-2 oncogene products in Reed–Sternberg cells independent of presence of Epstein–Barr virus. J Clin Pathol 46, 211–217.[CrossRef]
    [Google Scholar]
  18. Jiwa, N. M., Oudejans, J. J., Dukers, D. F., Vos, W., Horstman, A., van der Valk, P., Middeldorp, J. M., Walboomers, J. M. & Meijer, C. J. L. M. ( 1995; ). Immunohistochemical demonstration of different latent membrane protein-1 epitopes of Epstein–Barr virus in lymphoproliferative diseases. J Clin Pathol 48, 438–442.[CrossRef]
    [Google Scholar]
  19. Kievits, T., van Gemen, B., van Strijp, D., Schukkink, R., Dircks, M., Adriaanse, H., Malek, L., Sooknanan, R. & Lens, P. ( 1995; ). NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection. J Virol Methods 35, 273–286.
    [Google Scholar]
  20. McCall, S. A., Lichy, J. H., Bijwaard, K. E., Aguilera, N. S., Chu, W. S. & Taubenberger, J. K. ( 2001; ). Epstein–Barr virus detection in ductal carcinoma of the breast. J Natl Cancer Inst 93, 148–150.
    [Google Scholar]
  21. Oudejans, J. J., Jiwa, M., van den Brule, A. J. C. & 7 other authors ( 1995; ). Detection of heterogeneous Epstein–Barr virus gene expression patterns within individual post-transplantation lymphoproliferative disorders. Am J Pathol 147, 923–933.
    [Google Scholar]
  22. Parkin, D. M., Pisani, P. & Ferlay, J. ( 1999; ). Global cancer statistics. CA Cancer J Clin 49, 33–64.[CrossRef]
    [Google Scholar]
  23. Sbih-Lammali, F., Djennaoui, D., Belaoui, H., Bouguermouh, A., Decaussin, G. & Ooka, T. ( 1996; ). Transcriptional expression of Epstein–Barr virus genes and proto-oncogenes in north African nasopharyngeal carcinoma. J Med Virol 49, 7–14.[CrossRef]
    [Google Scholar]
  24. Smith, P. R., de Jesus, O., Turner, D. & 7 other authors ( 2000; ). Structure and coding content of CST (BART) family RNAs of Epstein–Barr virus. J Virol 74, 3082–3092.[CrossRef]
    [Google Scholar]
  25. Sugawara, Y., Mizugaki, Y., Uchida, T., Torii, T., Imai, S., Makuuchi, M. & Takada, K. ( 1999a; ). Detection of Epstein–Barr virus (EBV) in hepatocellular carcinoma tissue: a novel EBV latency characterized by the absence of EBV-encoded small RNA expression. Virology 256, 196–202.[CrossRef]
    [Google Scholar]
  26. Sugawara, Y., Makuuchi, M., Kato, N., Shimotohno, K. & Takada, K. ( 1999b; ). Enhancement of hepatitis C virus replication by Epstein–Barr virus encoded nuclear antigen 1. EMBO J 18, 5755–5760.[CrossRef]
    [Google Scholar]
  27. Sugawara, Y., Makuuchi, M. & Takada, K. ( 2000; ). Detection of Epstein–Barr virus DNA in hepatocellular carcinoma tissue from hepatitis C-positive patients. Scand J Gastroenterol 9, 981–984.
    [Google Scholar]
  28. van Beek, J., zur Hausen, A., Kranenbarg, E. K. & 7 other authors ( 2002; ). A rapid and reliable enzyme immunoassay PCR-based screening method to identify EBV-carrying gastric carcinomas. Mod Pathol 15, 870–877.[CrossRef]
    [Google Scholar]
  29. zur Hausen, A., Brink, A. A., Craanen, M. E., Middeldorp, J. M., Meijer, C. J. L. M. & van den Brule, A. J. C. ( 2000; ). Unique transcription pattern of Epstein–Barr virus (EBV) in EBV-carrying gastric adenocarcinomas: expression of the transforming BARF1 gene. Cancer Res 60, 2745–2748.
    [Google Scholar]
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