1887

Abstract

The multiple nucleopolyhedrovirus (RoMNPV) is a variant of MNPV (AcMNPV) but is significantly more virulent against several major agricultural pests. The genome sequence of the R1 strain of RoMNPV was determined and compared to that of AcMNPV strain C6. The RoMNPV genome is approximately 131·5 kbp with a G+C content of 39·1 %. The homologous repeat regions (s) described for AcMNPV-C6 are present in RoMNPV-R1 but the s of RoMNPV have fewer palindromic repeats. The RoMNPV-R1 nucleotide sequence is almost completely collinear with the sequence of AcMNPV-C6 and contains homologues of 150 of the 155 ORFs described for AcMNPV-C6. Deletions, insertions and substitutions have resulted in the loss of homologues for AcMNPV ORFs (), (), , and from the RoMNPV genome. The average amino acid sequence identity between RoMNPV and AcMNPV ORFs is 96·1 % and there are differences in promoter motif composition for 23 of these ORFs. Maximum-likelihood analysis of selection pressures on AcMNPV and RoMNPV ORFs indicate that ORFs / () and / () have undergone positive selection.

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2003-07-01
2019-10-20
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