1887

Abstract

Infection of cultured insect cells with multicapsid nucleopolyhedrovirus (SeMNPV) resulted in the generation of mutants with major genomic deletions. Some of the mutants lacked the ability to infect larvae . The gene(s) responsible for this phenotype in SeMNPV was mapped within a contiguous sequence encoding ORFs 29–35. In this paper we have shown that SeMNPV ORFs 15–35 (including genes encoding cathepsin, chitinase, GP37, PTPT-2, EGT, PKIP-1 and ARIF-1) are not essential for virus replication in cell culture or by intrahaemocoelic injection. By site-specific deletion mutagenesis of a full-length infectious clone of SeMNPV (bacmid) using ET recombination in , a series of SeMNPV bacmid mutants with increasing deletions in ORFs 15–35 was generated. Analyses of these mutants indicated that a deletion of SeMNPV ORF35 (Se35) resulted in loss of oral infectivity of polyhedral occlusion bodies. Reinsertion of ORF35 in SeMNPV bacmids lacking Se35 rescued oral infectivity. We propose the name for Se35 and its baculovirus homologues (e.g. MNPV ORF22), by analogy to a different gene recently characterized in NPV, which was designated infectivity factor (). Similar to the gene, which encodes an essential structural protein of the occlusion-derived virus envelope, and belong to a group of 30 genes that are conserved among the .

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2003-08-01
2020-01-21
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