1887

Abstract

A variant of the serpin squamous cell carcinoma antigen (SCCA) has been identified as a hepatitis B virus binding protein and high expression of SCCA has recently been found in hepatocarcinoma. Since HBV is involved in liver carcinogenesis, experiments were carried out to examine the effect of HBV preS1 envelope protein on SCCA expression. Surface and intracellular staining for SCCA was assessed by FACS analysis. Preincubation of HepG2 cells and primary human hepatocytes with preS1 protein or with preS1(21–47) tetrameric peptide significantly increased the surface expression of SCCA, without modification of its overall cellular burden, suggesting a surface redistribution of the serpin. An increase in HBV binding and internalization was observed after pre-incubation of the cells with preS1 preparations, compared to cells preincubated with medium alone. Pretreatment of cells with DMSO, while not influencing SCCA basal expression, was responsible for an increase in the efficiency of HBV internalization and this effect was additive to that obtained after incubation with preS1 preparations. In conclusion, the HBV preS1(21–47) sequence is able to induce overexpression of SCCA at the cell surface facilitating virus internalization, while the increased efficiency of HBV entry following DMSO addition is not mediated by SCCA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19130-0
2004-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/3/vir850621.html?itemId=/content/journal/jgv/10.1099/vir.0.19130-0&mimeType=html&fmt=ahah

References

  1. Beasley R. P., Lin C. C., Hwang L. Y., Chien C. S. 1981; Hepatocellular carcinoma and hepatitis B virus: a prospective study of 22,707 men in Taiwan. Lancet 2:1129–1133
    [Google Scholar]
  2. Bueinda M. A. 1998; Hepatitis B viruses and cancerogenesis. Biomed Pharmacother 52:34–43
    [Google Scholar]
  3. De Falco S., Ruvo M., Verdoliva A., Scarallo A., Raimondo D., Raucci A., Fassina G. 2001a; N-terminal myristylation of HBV preS1 domain enhances receptor recognition. J Pept Res 57:390–400
    [Google Scholar]
  4. De Falco S., Ruvoletto M. G., Verdoliva A. 8 other authors 2001b; Cloning and expression of a novel hepatitis B virus binding protein from HepG 2 cells. J Biol Chem 276:36613–36623
    [Google Scholar]
  5. De The H., Marchio A., Tiollais P., Dejean A. 1989; Differential expression and ligand regulation of the retinoic acid receptor alpha and beta gene. EMBO J 8:429–433
    [Google Scholar]
  6. Imbert V., Rezzonico R., Reichenbach P., Nabholz M. 2002; Induction of interleukin-2 receptor alpha (IL-2Ralpha) expression by interleukin-2: important role of the interleukin-2 receptor beta chain region between the two Stat5 docking sites. Eur Cytokine Netw 13:331–339
    [Google Scholar]
  7. Kato H. 1996; Expression and function of squamous cell carcinoma antigen. Anticancer Res 6:2149–2154
    [Google Scholar]
  8. Le Seyec J., Chouteau P., Cannie I., Gouguen-Guillouzo C., Gripon P. 1999; Infection process of the hepatitis B virus depends on the presence of a defined sequence in the pre-S1 domain. J Virol 73:2052–2057
    [Google Scholar]
  9. Mantero G., Zonaro A., Albertini A., Bertolo P., Primi D. 1991; DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction. Clin Chem 37:422–429
    [Google Scholar]
  10. Mills D., Clifford J., Pak S. C., Cataltepe S., Silverman G. A. 2002; Cell surface detection of SCCA protein. Proceedings of the 3 rd International Symposium on Serpin Biology Structure & Function Chicago: June 2nd–5th 2002 p– 89
    [Google Scholar]
  11. Neurath A. R., Kent S. B., Strick N., Parker K. 1986; Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:429–436
    [Google Scholar]
  12. Paran N., Geiger B., Shaul Y. 2001; HBV infection of cell culture: evidence for multivalent and cooperative attachment. EMBO J 20:4443–4453
    [Google Scholar]
  13. Pontisso P., Ruvoletto M. G., Gerlich W. H., Heermann K. H., Bardini R., Alberti A. 1989; Identification of an attachment site for human liver plasma membranes on hepatitis B virus particles. Virology 173:522–530
    [Google Scholar]
  14. Pontisso P., Calabrese F., Benvegnù L. 8 other authors 2003; Overexpression of squamous cell carcinoma antigen variants in hepatocellular carcinoma. Br J Cancer (in press)
    [Google Scholar]
  15. Rijntjes P. J., Moshage H. J., Van Gemert P. J., De Waal R., Yap S. H. 1986; Cryopreservation of adult human hepatocytes. The influence of deep freezing storage on the viability, cell seeding, survival, fine structures and albumin synthesis in primary cultures. J Hepatol 3:7–18
    [Google Scholar]
  16. Rumin S., Gripon P., Le Seyec J., Corral-Debrinski M., Guguen-Guillouzo C. 1996; Long-term productive episomal hepatitis B virus replication in primary cultures of adult hepatocyte infected in vitro. J Viral Hepat 3:227–238
    [Google Scholar]
  17. Suminami Y., Nawata S., Kato H. 1998; Biological role of SCC Antigen. Tumor Biol 19:488–493
    [Google Scholar]
  18. Uemura Y., Pak S. C., Luke C. 7 other authors 2000; Circulating serpin tumor markers SCCA and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells. Int J Cancer 89:368–377
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19130-0
Loading
/content/journal/jgv/10.1099/vir.0.19130-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error