Hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells Free

Abstract

Hantaviruses are maintained in nature in persistently infected rodents and can also persistently infect cultured mammalian cells, causing little or no cytopathology. An unexpected outcome of this study was the observation of cytopathic effects (CPE) in the hantavirus-infected human embryonic kidney cell line HEK293. It was confirmed that hantaviruses induce apoptosis in HEK293 cells, although apoptosis appeared mostly in uninfected, bystander cells and rarely in infected HEK293 cells. Although studies by others suggest that the nucleocapsid protein of Puumala virus interacts with the Fas-mediated apoptosis enhancer Daxx at the gene expression level, it was determined that members of the TNF receptor superfamily did not contribute to the apoptosis observed in infected HEK293 cells. The observation of CPE in HEK293 cells might lead to a better understanding of the mechanisms of persistence and pathogenesis in hantavirus infections.

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2003-08-01
2024-03-29
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References

  1. Arikawa J., Takashima I., Hashimoto N. 1985; Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralizing antibody. Arch Virol 86:303–313
    [Google Scholar]
  2. Arikawa J., Schmaljohn A. L., Dalrymple J. M., Schmaljohn C. S. 1989; Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. J Gen Virol 70:615–624
    [Google Scholar]
  3. Bodary S. C., McLean J. W. 1990; The integrin β 1 subunit associates with the vitronectin receptor α v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem 265:5938–5941
    [Google Scholar]
  4. Brassard D. L., Maxwell E., Malkowski M., Nagabhushan T. L., Kumar C. C., Armstrong L. 1999; Integrin α v β 3-mediated activation of apoptosis. Exp Cell Res 251:33–45
    [Google Scholar]
  5. Brown M., Petric M. 1986; Evaluation of cell line 293 for virus isolation in routine viral diagnosis. J Clin Microbiol 23:704–708
    [Google Scholar]
  6. Collins J. A., Schandi C. A., Young K. K., Vesely J., Willingham M. C. 1997; Major DNA fragmentation is a late event in apoptosis. J Histochem Cytochem 45:923–934
    [Google Scholar]
  7. Everett H., McFadden G. 1999; Apoptosis: an innate immune response to virus infection. Trends Microbiol 7:160–165
    [Google Scholar]
  8. Gavrilovskaya I. N., Shepley M., Shaw R., Ginsberg M. H., Mackow E. R. 1998; β 3 integrins mediate the cellular entry of hantaviruses that cause respiratory failure. Proc Natl Acad Sci U S A 95:7074–7079
    [Google Scholar]
  9. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 36:59–74
    [Google Scholar]
  10. Hensley L. E., Young H. A., Jahrling P. B., Geisbert T. W. 2002; Proinflammatory response during Ebola virus infection of primate models: possible involvement of the tumor necrosis factor receptor superfamily. Immunol Lett 80:169–179
    [Google Scholar]
  11. Hooper J. W., Larsen T., Custer D. M., Schmaljohn C. S. 2001; A lethal disease model for hantavirus pulmonary syndrome. Virology 289:6–14
    [Google Scholar]
  12. Johnson A. M., Bowen M. D., Ksiazek T. G., Williams R. J., Bryan R. T., Mills J. N., Peters C. J., Nichol S. T. 1997; Laguna Negra virus associated with HPS in western Paraguay and Bolivia. Virology 238:115–127
    [Google Scholar]
  13. Kanerva M., Mustonen J., Vaheri A. 1998; Pathogenesis of Puumala and other hantavirus infections. Rev Med Virol 8:67–86
    [Google Scholar]
  14. Kang J. I., Park S. H., Lee P. W., Ahn B. Y. 1999; Apoptosis is induced by hantaviruses in cultured cells. Virology 264:99–105
    [Google Scholar]
  15. Kaplan D., Sieg S. 1998; Role of the Fas/Fas ligand apoptotic pathway in human immunodeficiency virus type 1 disease. J Virol 72:6279–6282
    [Google Scholar]
  16. Khaiboullina S. F., St Jeor S. C. 2002; Hantavirus immunology. Viral Immunol 15:609–625
    [Google Scholar]
  17. Kitamura T., Morita C., Komatsu T. 9 other authors 1983; Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system. Jpn J Med Sci Biol 36:17–25
    [Google Scholar]
  18. Larregina A. T., Morelli A. E., Dewey R. A., Castro M. G., Fontana A., Lowenstein P. R. 1998; FasL induces Fas/Apo1-mediated apoptosis in human embryonic kidney 293 cells routinely used to generate E1-deleted adenoviral vectors. Gene Ther 5:563–568
    [Google Scholar]
  19. Lee H. W., Lee P. W., Johnson K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. J Infect Dis 137:298–308
    [Google Scholar]
  20. Li X. D., Mäkela T. P., Guo D., Soliymani R., Koistinen V., Vapalahti O., Vaheri A., Lankinen H. 2002; Hantavirus nucleocapsid protein interacts with the Fas-mediated apoptosis enhancer Daxx. J Gen Virol 83:759–766
    [Google Scholar]
  21. Markotic A., Rabatic S., Gagro A., Sabioncello A., Kuzman I., Dekaris D. 1999; Immunopathogenesis of hemorrhagic fever with renal syndrome (HFRS) in humans. In Emergence and Control of Rodent-Borne Viral Diseases (Hantaviruses and Arenaviruses) pp  125–131 Annecy, France: Les Pensières;
    [Google Scholar]
  22. McCaughey C., Shi X., Elliot R. M., Wyatt D. E., O'Neill H. J., Coyle P. V. 1999; Low pH-induced cytopathic effect: a survey of seven hantavirus strains. J Virol Methods 81:193–197
    [Google Scholar]
  23. Meyer B. J., Schmaljohn C. S. 2000; Persistent hantavirus infections: characteristics and mechanisms. Trends Microbiol 8:61–67
    [Google Scholar]
  24. O'Brien V. 1998; Viruses and apoptosis. J Gen Virol 79:1833–1845
    [Google Scholar]
  25. Schmaljohn C., Hjelle B. 1997; Hantaviruses: a global disease problem. Emerg Infect Dis 3:95–104
    [Google Scholar]
  26. Schmaljohn A. L., Li D., Negley D. L., Bressler D. S., Turell M. J., Korch G. W., Ascher M. S., Schmaljohn C. S. 1995; Isolation and initial characterization of a newfound hantavirus from California. Virology 206:963–972
    [Google Scholar]
  27. Toro J., Vega J. D., Khan A. S. 20 other authors 1998; An outbreak of hantavirus pulmonary syndrome. Chile: 1997 Emerg Infect Dis 4:687–694
    [Google Scholar]
  28. Tukey J. W. 1949; Comparing individual means in the analysis of variance. Biometrics 5:99–114
    [Google Scholar]
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