1887

Abstract

The gene () of M nucleopolyhedrovirus encodes the largest subunit of the virally encoded DNA-directed RNA polymerase specific for the transcription of late and very late viral genes. The sequence of predicts a C-terminal motif of 13 amino acids that is conserved in other polymerases. Detailed mutagenesis throughout was performed, including this C-terminal motif, to define sequences required for late promoter activation. It was found that the conserved C-terminal motif was critical for late gene expression. In addition, regions throughout the entire -encoding sequence were important for optimal function, suggesting complex protein–protein and protein–DNA interrelationships in the late gene-specific viral transcriptosome.

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2003-07-01
2019-10-17
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