@article{mbs:/content/journal/jgv/10.1099/vir.0.19074-0, author = "Bose, Santanu and Mathur, Manjula and Bates, Patricia and Joshi, Nikita and Banerjee, Amiya K.", title = "Requirement for cyclophilin A for the replication of vesicular stomatitis virus New Jersey serotype", journal= "Journal of General Virology", year = "2003", volume = "84", number = "7", pages = "1687-1699", doi = "https://doi.org/10.1099/vir.0.19074-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19074-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Several host proteins have been shown to play key roles in the life-cycle of vesicular stomatitis virus (VSV). We have identified an additional host protein, cyclophilin A (CypA), a chaperone protein possessing peptidyl cis-trans prolyl-isomerase activity, as one of the cellular factors required for VSV replication. Inhibition of the enzymatic activity of cellular CypA by cyclosporin A (CsA) or SDZ-211-811 resulted in a drastic inhibition of gene expression by VSV New Jersey (VSV-NJ) serotype, while these drugs had a significantly reduced effect on the genome expression of VSV Indiana (VSV-IND) serotype. Overexpression of a catalytically inactive mutant of CypA resulted in the reduction of VSV-NJ replication, suggesting a requirement for functional CypA for VSV-NJ infection. It was also shown that CypA interacted with the nucleocapsid (N) protein of VSV-NJ and VSV-IND in infected cells and was incorporated into the released virions of both serotypes. VSV-NJ utilized CypA for post-entry intracellular primary transcription, since inhibition of CypA with CsA reduced primary transcription of VSV-NJ by 85–90 %, whereas reduction for VSV-IND was only 10 %. Thus, it seems that cellular CypA binds to the N protein of both serotypes of VSV. However, it performs an obligatory function on the N protein activity of VSV-NJ, while its requirement is significantly less critical for VSV-IND N protein function. The different requirements for CypA by two serologically different viruses belonging to the same family has highlighted the utilization of specific host factors during their evolutionary lineages.", }