RT Journal Article SR Electronic(1) A1 Suzuki, Masashi A1 Yoshida, Megumi A1 Yoshinuma, Toshio A1 Hibi, TadaakiYR 2003 T1 Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus JF Journal of General Virology, VO 84 IS 7 SP 1931 OP 1939 DO https://doi.org/10.1099/vir.0.19070-0 PB Microbiology Society, SN 1465-2099, AB Cucumber mosaic virus (CMV) and Peanut stunt virus (PSV) each have genomes consisting of three single-stranded RNA molecules: RNA 1, 2 and 3. RNAs 1 and 2 encode the 1a and 2a proteins, respectively, which are necessary for replication of the viral genome. Although RNA 3 is exchangeable between CMV and PSV, exchange of RNA 1 and 2 between the two viruses has been unsuccessful. In this study, reassortants containing PSV RNA 1 and CMV RNA 2 together with RNA 3 of CMV or PSV were shown to be able to replicate their genomic RNA, but not to transcribe subgenomic RNA 4 in tobacco protoplasts. Conversely, the reassortant consisting of CMV RNA 1 and PSV RNA 2 together with RNA 3 of CMV or PSV could not replicate. Subsequently, a yeast two-hybrid system was used to analyse the in vivo interaction between the 1a and 2a proteins. The C-terminal half of PSV-1a protein interacted with the N-terminal region of 2a protein of both PSV and CMV, but the C-terminal half of CMV-1a and the N-terminal region of PSV-2a did not interact. These results suggest that RNA replication in the interspecific reassortant between CMV and PSV requires compatibility between the C-terminal half of the 1a protein and the N-terminal region of the 2a protein, and this compatibility is insufficient for transcription of subgenomic RNA 4., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19070-0