1887

Abstract

Multipartite nucleic acid-containing virus-like particles, known as polydnaviruses, are special structures produced by female parasitoid wasps to deliver wasp components into the body of their host at oviposition. The particles confer protection for the developing parasitoid by passive and active means. Although several genes expressed from the circular DNA of these particles have been identified from various host–parasitoid systems, there is not much known about the structural proteins of these particles. Here we report on two genes encoding particle proteins with similarities to molecular chaperones, calreticulin and heat-shock protein 70.

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2003-05-01
2020-07-14
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References

  1. Asgari S., Schmidt O.. 1994; Passive protection of eggs from the parasitoid, Cotesia rubecula , in the host Pieris rapae . J Insect Physiol40:789–795
    [Google Scholar]
  2. Asgari S., Hellers M., Schmidt O.. 1996; Host haemocyte inactivation by an insect parasitoid: transient expression of a polydnavirus gene. J Gen Virol77:2653–2662
    [Google Scholar]
  3. Asgari S., Theopold U., Wellby C., Schmidt O.. 1998; A protein with protective properties against the cellular defence reactions in insects. Proc Natl Acad Sci U S A95:3690–3695
    [Google Scholar]
  4. Beckage N. E.. 1998; Modulation of immune responses to parasitoids by polydnaviruses. Parasitology116:S57–64
    [Google Scholar]
  5. Beckage N. E., Tan F. F., Schleifer K. W., Lane R. D., Cherubin L. L.. 1994; Characterization and biological effects of Cotesia congregata polydnavirus on host larvae of the tobacco hornworm, Manduca sexta . Arch Insect Biochem Physiol26:165–195
    [Google Scholar]
  6. Cavener R. C., Ray S. C.. 1991; Eukaryotic start and stop translation sites. Nucleic Acids Res19:3185–3192
    [Google Scholar]
  7. Choi J. Y., Whitten M. M. A., Cho M. Y., Lee K. Y., Kim M. S., Ratcliffe N. A., Lee B. A.. 2002; Calreticulin enriched as an early-stage encapsulation protein in wax moth Galleria mellonella larvae. Dev Comp Immunol26:335–343
    [Google Scholar]
  8. Chomczynski P., Sacchi N.. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem162:156–159
    [Google Scholar]
  9. Gurer C., Cimarelli A., Luban J.. 2002; Specific incorporation of heat shock protein 70 family members into primate lentiviral virions. J Virol76:4666–4670
    [Google Scholar]
  10. Jaubert S., Ledger T. N., Laffaire J. B., Piotte C., Abad P., Rosso M.-N.. 2002; Direct identification of stylet secreted proteins from root-knot nematodes by a proteomics approach. Mol Biochem Parasitol121:205–211
    [Google Scholar]
  11. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  12. Michalak M., Milner R. E., Burns K., Opas M.. 1992; Calreticulin. Biochem J285:681–692
    [Google Scholar]
  13. Nakhasi H. L., Pogue G. P., Duncan R. C., Joshi M., Atreya C. D., Lee N. S., Dwyer D. M.. 1998; Implication of calreticulin function in parasite biology. Parasitol Today14:157–160
    [Google Scholar]
  14. Opas M., Tharin S., Milner R. E., Michalak M.. 1996; Identification and localization of calreticulin in plant cells. Protoplasma191:164–171
    [Google Scholar]
  15. Pritchard D. I., Brown A., Kasper G., Mcelroy P., Loukas A., Hewitt C., Berry C., Fullkrug R., Beck E.. 1999; A hookworm allergen which strongly resembles calreticulin. Parasite Immunol21:439–450
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Smith D. E., Fisher A. P.. 1984; Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots. J Cell Biol99:20–28
    [Google Scholar]
  18. Spiro R. G., Zhu Q., Bhoyroo V., Söling H.-D.. 1996; Definition of the lectin-like properties of the molecular chaperon, calreticulin, and demonstration of its copurification with endomannosidase from rat liver Golgi. J Biol Chem271:11588–11594
    [Google Scholar]
  19. Stoltz D. B.. 1990; Evidence for chromosomal transmission of polydnavirus DNA. J Gen Virol71:1051–1056
    [Google Scholar]
  20. Strand M. R., McKenzie V., Grassl B. A., Aiken J. M.. 1992; Persistence and expression of Microplitis demolitor polydnavirus in Pseudoplusia includens . J Gen Virol73:1627–1635
    [Google Scholar]
  21. Sullivan C. S., Pipas J. M.. 2001; The virus–chaperone connection. Virology287:1–8
    [Google Scholar]
  22. Webb B. A.. 1998; Polydnavirus biology, genome structure, and evolution. In The Insect Viruses pp 105–139 Edited by Miller L. K., Ball L. A.. New York: Plenum;
    [Google Scholar]
  23. Whitfield J. B., Asgari S.. 2002; Virus or not? Phylogenetics of polydnaviruses and their wasp carriers. J Insect Physiol (in press)
    [Google Scholar]
  24. Yao L., Pike S. E., Tosato G.. 2002; Laminin binding to the calreticulin fragment vasostatin regulates endothelial cell function. J Leukoc Biol71:47–53
    [Google Scholar]
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