1887

Abstract

Apoptosis was detected in Vero cell cultures expressing transfected dengue virus type 2 (DENV-2) genes. Approximately 17·5 and 51·5 % of cells expressing NS3 serine protease and NS2B-NS3 serine protease precursor protein [NS2B-NS3(pro)] genes, respectively, were apoptotic. The percentage of apoptotic cells was significantly higher in cell cultures expressing NS2B-NS3(pro). NS2B-NS3(pro) was detected as NS2B-NS3(pro)-EGFP fusion protein in cytoplasmic vesicular structures in the apoptotic cells. Site-directed mutagenesis which replaced His with Ala within the protease catalytic triad significantly reduced the ability of the expressed NS3 and NS2B-NS3(pro) to induce apoptosis. Results from the present study showed that DENV-2-encoded NS3 serine protease induces apoptosis, which is enhanced in cells expressing its precursor, NS2B-NS3(pro). These findings suggest the importance of NS2B as a cofactor to NS3 protease-induced apoptosis.

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2003-08-01
2020-05-25
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