1887

Abstract

White spot syndrome virus (WSSV) is a member of a new virus family () infecting crustaceans. The regulation of transcription of WSSV genes is largely unknown. Transcription of the major WSSV structural virion protein genes, , , , and , was studied to search for common promoter motifs for coordinate expression. The temporal expression of these genes and both 5′ and 3′ ends of the mRNA were determined, using infected crayfish gill tissue as a RNA source. RT-PCR showed that all five genes are expressed late in infection compared to the early ribonucleotide reductase large subunit gene. 5′ RACE studies revealed a consensus late transcription initiation motif for only two of the five major virion protein genes. This motif was only found in one other upstream region of the putative translational start site of a gene with unknown function (ORF 158). No other conserved sequence motifs could be detected in the sequences surrounding the transcriptional start sites of the five major virion protein genes. All 5′ ends were located about 25 nt downstream of an A/T rich sequence, including the consensus TATA-box sequence for . The absence of a consensus motif is distinct from gene regulation of other large dsDNA viruses and suggests a unique regulation of WSSV transcription, in line with its unique taxonomic position.

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2003-06-01
2024-12-06
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References

  1. Burma S., Mukherjee B., Jain A., Habib S., Hasnain S. 1994; An unusual 30-kDa protein binding to the polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus. J Biol Chem 269:2750–2757
    [Google Scholar]
  2. Chen L. L., Wang H. C., Huang C. J. 9 other authors 2002; Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology 301:136–147
    [Google Scholar]
  3. Davison A. J., Moss B. 1989; Structure of vaccinia virus late promoters. J Mol Biol 210:771–784
    [Google Scholar]
  4. Durand S., Lightner D. V., Redman R. M., Bonami J. R. 1997; Ultrastructure and morphogenesis of white spot syndrome baculovirus (WSSV). Dis Aquat Org 29:205–211
    [Google Scholar]
  5. Fitzgerald M., Shenk T. 1981; The sequence 5′-AAUAAA-3′ forms parts of the recognition site for polyadenylation of late SV40 mRNAs. Cell 24:251–260
    [Google Scholar]
  6. Garcia-Escudero R., Viñuela E. 2000; Structure of African swine fever virus late promoters: requirement of a TATA sequence at the initiation region. J Virol 74:8176–8182
    [Google Scholar]
  7. Huang C., Zhang X., Lin Q., Xu X., Hu Z., Hew C. L. 2002; Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelope protein VP466. Mol Cell Proteomics 1:223–231
    [Google Scholar]
  8. Kim D. B., Zabierowski S., DeLuca N. A. 2002; The initiator element in a herpes simplex virus type 1 late-gene promoter enhances activation by ICP4, resulting in abundant late-gene expression. J Virol 76:1548–1558
    [Google Scholar]
  9. Kozak M. 1989; The scanning model for translation: an update. J Cell Biol 108:229–241
    [Google Scholar]
  10. Liu W. J., Yu H. T., Peng S. E. 10 other authors 2001; Cloning, characterization, and phylogenetic analysis of a shrimp white spot syndrome virus gene that encodes a protein kinase. Virology 289:362–377
    [Google Scholar]
  11. Morris T. D., Miller L. K. 1994; Mutational analysis of a baculovirus major late promoter. Gene 140:147–153
    [Google Scholar]
  12. Nadala E. C. Jr, Tapay L. M., Loh P. C. 1998; Characterization of a non-occluded baculovirus-like agent pathogenic to penaeid shrimp. Dis Aquat Org 33:221–229
    [Google Scholar]
  13. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; GeneDoc: analysis and visualization of genetic variation. EMBNEW.NEWS 4:14
    [Google Scholar]
  14. Tsai M. F., Lo C. F., van Hulten M. C. W. & 7 other authors (2000a). Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus. Virology 277:92–99
    [Google Scholar]
  15. Tsai M. F., Yu H. T., Tzeng H. F. 7 other authors 2000b; Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology 277:100–110
    [Google Scholar]
  16. van Hulten M. C. W., Westenberg M., Goodall S. D., Vlak J. M. 2000a; Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology 266:227–236
    [Google Scholar]
  17. van Hulten M. C. W., Goldbach R. W., Vlak J. M. 2000b; Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication. J Gen Virol 81:2525–2529
    [Google Scholar]
  18. van Hulten M. C. W., Witteveldt J., Peters S., Kloosterboer N., Tarchini R., Fiers M., Sandbrink H., Klein Lankhorst R., Vlak J. M. 2001a; The white spot syndrome virus DNA genome sequence. Virology 286:7–22
    [Google Scholar]
  19. van Hulten M. C. W., Witteveldt J., Snippe M., Vlak J. M. 2001b; White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology 285:228–233
    [Google Scholar]
  20. van Hulten M. C. W., Reijns M., Vermeesch A. M. G., Zandbergen F., Vlak J. M. 2002; Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J Gen Virol 83:257–265
    [Google Scholar]
  21. Wang Y. C., Lo C. F., Chang P. S., Kou G. H. 1998; Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture 164:221–231
    [Google Scholar]
  22. Weir J. P. 2001; Regulation of herpes simplex virus gene expression. Gene 271:117–130
    [Google Scholar]
  23. Wongteerasupaya C., Vickers J. E., Sriurairatana S. 7 other authors 1995; A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon . Dis Aquat Org 21:69–77
    [Google Scholar]
  24. Yang F., He J., Lin X. H., Li Q., Pan D., Zhang X., Xu X. 2001; Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811–11820
    [Google Scholar]
  25. Zhang X., Xu X., Hew C. L. 2001; The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus. Virus Res 79:137–144
    [Google Scholar]
  26. Zhang X., Huang C. H., Xu X., Hew C. L. 2002; Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus. J Gen Virol 83:471–477
    [Google Scholar]
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