1887

Abstract

Infectivity of the cloned DNA genome of strawberry vein banding virus (SVBV) was demonstrated by particle bombardment of 4-week-old strawberry ( L. var. UC-5) plants with gold particles coated with the putative full-length 7·9 kb viral DNA. Vein banding symptoms developed on 15 % of inoculated plants 6–7 weeks post-inoculation. An approximate 1·25-mer of the viral DNA was cloned into the binary vector pCGN1547. Particle bombardment of this construct into strawberry plants gave an infection rate of 75 %. The construct was used for transformation of , and infiltration of these cells into healthy strawberry leaves resulted in development of vein banding symptoms in 100 % of inoculated plants. Gel electrophoresis, Southern blot hybridization with an SVBV probe and sequence analyses of PCR-amplified DNA fragments were used to confirm SVBV infection in symptomatic plants.

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2003-06-01
2021-10-23
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References

  1. Frazier N. W. 1955; Strawberry vein banding virus. Phytopathology 45:307–312
    [Google Scholar]
  2. Gal S., Pisan B., Hohn T., Grimsley N., Hohn B. 1992; Agroinfection of transgenic plants leads to viable cauliflower mosaic virus by intermolecular recombination. Virology 187:525–533
    [Google Scholar]
  3. Hou Y.-M., Paplomatas E. J., Gilbertson R. L. 1998; Host adaptation and replication properties of two bipartite geminiviruses and their pseudorecombinants. Mol Plant Microbe Interact 11:208–217
    [Google Scholar]
  4. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. J Gen Virol 31:93–100
    [Google Scholar]
  5. Kitajima E. W., Betti J. A., Costa A. S. 1973; Strawberry vein-banding virus, a member of the cauliflower mosaic virus group. J Gen Virol 20:117–119
    [Google Scholar]
  6. Lamprecht S., Jelkmann W. 1997; Infectious cDNA clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the disease. J Gen Virol 78:2347–2353
    [Google Scholar]
  7. Mahmoudpour M. M. A. 2000; Strawberry vein banding caulimovirus: biology and characterization . PhD dissertation University of California; Davis, CA, USA:
  8. McBride K. E., Summerfelt K. R. 1990; Improved binary vectors for Agrobacterium -mediated plant transformation. Plant Mol Biol 14:269–276
    [Google Scholar]
  9. Miltenburg R. V., Rüger B., Grünewald-Janho S., Leons M., Schröder C. 1995 The Dig System User's Guide for Filter Hybridization Indianapolis: Boehringer Mannheim;
    [Google Scholar]
  10. Morris T. J., Mullin R. H., Sclegel D. E., Cole A., Alosi M. C. 1980; Isolation of a caulimovirus from strawberry tissue infected with strawberry vein banding virus. Phytopathology 70:156–160
    [Google Scholar]
  11. Paplomatas E. J., Patel V. P., Hou Y.-M., Noueiry A. O., Gilbertson R. L. 1994; Molecular characterization of a new sap-transmissible bipartite genome geminivirus infecting tomatoes in Mexico. Phytopathology 84:1215–1224
    [Google Scholar]
  12. Petrzik K., Benes V., Mráz I., Honetšlegrová-Fránová J., Ansorge W., Špak J. 1998; Strawberry vein banding virus – definitive member of the genus caulimovirus. Virus Genes 16:303–305
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Stenger D. C., Mullin R. H., Morris T. J. 1988; Isolation, molecular cloning, and detection of strawberry vein banding virus DNA. Phytopathology 78:154–159
    [Google Scholar]
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