1887

Abstract

Infectivity of the cloned DNA genome of strawberry vein banding virus (SVBV) was demonstrated by particle bombardment of 4-week-old strawberry ( L. var. UC-5) plants with gold particles coated with the putative full-length 7·9 kb viral DNA. Vein banding symptoms developed on 15 % of inoculated plants 6–7 weeks post-inoculation. An approximate 1·25-mer of the viral DNA was cloned into the binary vector pCGN1547. Particle bombardment of this construct into strawberry plants gave an infection rate of 75 %. The construct was used for transformation of , and infiltration of these cells into healthy strawberry leaves resulted in development of vein banding symptoms in 100 % of inoculated plants. Gel electrophoresis, Southern blot hybridization with an SVBV probe and sequence analyses of PCR-amplified DNA fragments were used to confirm SVBV infection in symptomatic plants.

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2003-06-01
2020-01-18
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