Delineation of sequences important for efficient packaging of feline immunodeficiency virus RNA Free

Abstract

We have used systematic deletion analysis of the 5′ untranslated region (UTR) of the feline immunodeficiency virus (FIV) genome, both in the presence and absence of various amounts of , to define the -acting sequences responsible for efficient RNA packaging. Our analyses revealed that the primary FIV packaging signal consists of two essential core elements located within the first 90–120 bp of the 5′UTR and the first 90 bp of the gene. Interestingly, the region between the major splice donor (SD) and , including ∼130–160 bp upstream of the SD, is dispensable for encapsidation. Finally, other determinants of packaging were found to be present in the viral LTR and/or within the 3′ end of the viral genome. Taken together, our results suggest that the primary packaging determinants of FIV are multipartite and discontinuous, composed of two elements within the 5′UTR and gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18886-0
2003-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/3/vir840621.html?itemId=/content/journal/jgv/10.1099/vir.0.18886-0&mimeType=html&fmt=ahah

References

  1. Browning M. T., Schmidt R. D., Lew K. A., Rizvi T. A. 2001; Primate and feline lentiviral vector RNA packaging and propagation by heterologous lentiviral virions. J Virol 75:5129–5140
    [Google Scholar]
  2. Harrich D., Hooker C. W., Parry E. 2000; The human immunodeficiency virus type 1 TAR RNA upper stem–loop plays distinct roles in reverse transcription and RNA packaging. J Virol 74:5639–5646
    [Google Scholar]
  3. Johnston J. C., Gasmi M., Lim L. E., Elder J. H., Yee J.-K., Jolly D. J., Campbell K. P., Davidson B. L., Sauter S. 1999; Minimum requirement for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors. J Virol 73:4991–5000
    [Google Scholar]
  4. Kim H.-K., Lee K., O'Rear J. J. 1994; A short sequence upstream of the 5′ major splice site is important for encapsidation of HIV-1 genomic RNA. Virology 198:336–340
    [Google Scholar]
  5. McBride M. S., Panganiban A. T. 1996; The human immunodeficiency virus type 1 encapsidation site is a multipartite RNA element composed of functional hairpin structures. J Virol 70:2963–2973
    [Google Scholar]
  6. McBride M. S., Panganiban A. T. 1997; Position dependence of functional hairpins important for human immunodeficiency virus type 1 encapsidation in vivo. J Virol 71:2050–2058
    [Google Scholar]
  7. McBride M. S., Schwartz M. D., Panganiban A. T. 1997; Efficient encapsidation of human immunodeficiency virus type 1 vectors and further characterization of cis elements required for encapsidation. J Virol 71:4544–4554
    [Google Scholar]
  8. Poeschla E. M., Wong-Staal F., Looney D. J. 1998; Efficient transduction of nondividing cells by feline immunodeficiency virus lentiviral vectors. Nat Med 4:354–357
    [Google Scholar]
  9. Rizvi T. A., Panganiban A. T. 1993; Simian immunodeficiency virus RNA is efficiently encapsidated by human immunodeficiency virus type 1 particles. J Virol 67:2681–2688
    [Google Scholar]
  10. Schmidt R. S., Mustafa F., Lew K. A., Browning M. T., Rizvi T. A. 2003; Sequences both within the 5′ untranslated region and the gag gene are important for efficient encapsidation of Mason–Pfizer monkey virus RNA. Virology (in Press)
    [Google Scholar]
  11. Sorge J., Ricci W., Hughes S. H. 1983; Cis -acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus. J Virol 48:667–675
    [Google Scholar]
  12. Yu S. S., Kim J.-M., Kim S. 2000; The 17 nucleotides downstream from the env gene stop codon are important for murine leukemia virus packaging. J Virol 74:8775–8780
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18886-0
Loading
/content/journal/jgv/10.1099/vir.0.18886-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed