1887

Abstract

Entry of Japanese encephalitis virus (JEV) into cells was analysed by using the vertebrate cell line Vero. Vero cells were treated with chlorpromazine, nystatin or cytochalasin D, which inhibit clathrin- and caveola-dependent endocytosis, and macropinocytosis of the cells, respectively. Productive JEV infection was inhibited by pretreatment with chlorpromazine; the number of JEV antigen-positive cells was less than one-fifth of that in untreated cultures, but was not significantly decreased by pretreatment with nystatin or cytochalasin. Viral antigens were detected in the membrane fractions, but not in the endosome fractions from chlorpromazine-treated JEV-inoculated cells. When the cells were treated with chlorpromazine, clathrin heavy chain antigen and JEV antigen were not detected in cytoplasm by indirect immunofluorescence staining. These results indicate that JEV is taken up by cells through the clathrin-dependent endocytic pathway, and this process leads to infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18883-0
2003-07-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/7/vir841737.html?itemId=/content/journal/jgv/10.1099/vir.0.18883-0&mimeType=html&fmt=ahah

References

  1. Anderson R. G. W. 1993; Caveolae: where incoming and outgoing messengers meet. Proc Natl Acad Sci U S A 90:10909–10913
    [Google Scholar]
  2. Brown M. S., Anderson R. G. W., Goldstein J. L. 1983; Recycling receptors: the round-trip itinerary of migrant membrane proteins. Cell 32:663–667
    [Google Scholar]
  3. Butrapet S., Kimura-Kuroda J., Zhou D. S., Yasui K. 1998; Neutralizing mechanism of a monoclonal antibody against Japanese encephalitis virus glycoprotein E. Am J Trop Med Hyg 58:389–398
    [Google Scholar]
  4. Gentry M. K., Henchal E. A., McCown J. M., Brandt W. E., Dalrymple J. M. 1982; Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. Am J Trop Med Hyg 31:548–555
    [Google Scholar]
  5. Gollins S. W., Porterfield J. S. 1986; The uncoating and infectivity of the flavivirus West Nile on interaction with cells: effects of pH and ammonium chloride. J Gen Virol 67:1941–1950
    [Google Scholar]
  6. Heinz F. X., Allison S. L. 2001; Structures and mechanisms in flavivirus fusion. Adv Virus Res 55:231–269
    [Google Scholar]
  7. Ishak R., Tovey D. G., Howard C. R. 1988; Morphogenesis of yellow fever virus 17D in infected cell cultures. J Gen Virol 69:325–335
    [Google Scholar]
  8. Kimura T., Gollins S. E., Porterfield J. S. 1986; The effect of pH on the early interaction of West Nile virus with P388D1 cells. J Gen Virol 67:2423–2433
    [Google Scholar]
  9. Kimura-Kuroda J., Yasui K. 1986; Antigenic comparison of envelope protein E between Japanese encephalitis virus and some other flaviviruses using monoclonal antibodies. J Gen Virol 67:2663–2672
    [Google Scholar]
  10. Lisanti M. P., Tang Z., Sargiacomo M. 1993; Caveolin forms a heterooligomeric protein complex that interacts with an apical GPI-linked protein: implications for the biogenesis of caveolae. J Cell Biol 123:595–604
    [Google Scholar]
  11. Maniak M. 2001; Macropinocytosis. In Endocytosis pp  78–93 Edited by Marsh M. Oxford: Oxford University Press;
    [Google Scholar]
  12. Nawa M. 1996; Stability of hemagglutinating activity of extracellular and intracellular forms of Japanese encephalitis virus exposed to acidic pH. Microbiol Immunol 40:365–371
    [Google Scholar]
  13. Nawa M. 1997; Japanese encephalitis virus infection in Vero cells: the involvement of intracellular acidic vesicles in the early phase of viral infection was observed with the treatment of a specific vacuolar type H+-ATPase inhibitor, bafilomycin A1. Microbiol Immunol 41:537–543
    [Google Scholar]
  14. Ng M. L., Lau L. C. L. 1988; Possible involvement of receptors in the entry of Kunjin virus into Vero cells. Arch Virol 100:199–211
    [Google Scholar]
  15. Rothberg K. G., Ying Y. S., Kamen B. A., Anderson R. G. W. 1990; Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate. J Cell Biol 111:2931–2938
    [Google Scholar]
  16. Sieczkarski S. B., Whittaker G. R. 2002; Dissecting virus entry via endocytosis. J Gen Virol 83:1535–1545
    [Google Scholar]
  17. van Renswoude J., Bridges K. R., Harford J. B., Klausner R. D. 1982; Receptor-mediated endocytosis of transferrin and the uptake of Fe in K562 cells: identification of a nonlysosomal acidic compartments. Proc Natl Acad Sci U S A 79:6186–6190
    [Google Scholar]
  18. Wang L. H., Rothberg K. G., Anderson R. G. W. 1993; Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation. J Cell Biol 123:1107–1117
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18883-0
Loading
/content/journal/jgv/10.1099/vir.0.18883-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error